WormMine: ExpressionPattern Expr12251

• Pattern: For each of the mir::GFP-pest reporters (Plin-4::GFP-pest, Plet-7::GFP-pest, mir-1::GFP-pest) post-embryonic GFP-pest expression was first detected at approximately 14 hours post L1 arrest. Once transcriptionally activated, Plin-4::GFP-pest and Plet-7::GFP-pest reporters peak in expression by 18-20 hours and diminish with similar kinetics. For animals expressing the Plet-7::GFP-pest reporter GFP-pest expression was monitored for longer periods after release from L1 arrest. Consistent with the highly pulsatile nature of this expression pattern, GFP-pest expression was reinitiated at 30 hours, which correlates with the later portions of the L2 stage. While transcriptional activation of the Pmir-1::GFP-pest reporter was also initiated at 14 hours post-L1 arrest, the peak of Pmir-1::GFPpest expression occurred at a later time point, and diminished with slower kinetics, as compared to Plin-4::GFP-pest and Plet-7::GFP-pest expression.The majority of animals which harbor the Plin-4::GFP-pest transgene cease GFP-pest expression by L3 ecdysis and resume expression by the mid-L4 stage. The pulse of Plin-4::GFP-pest expression at the L4 stage extends through the early portion of young adulthood and completely overlaps with the lethargus period in all animals. Plet-7::GFP-pest expression followed a similar pattern. However, GFP-pest expression was more variable at the L3-to-L4 transition and L4-specific induction of this transgene was primarily restricted to the lethargus period. In contrast to the expression profiles of the lin-4 and let-7 reporters, induction of Pmir-1::GFP-pest expression began during, or immediately after, L3 ecdysis and persisted into the L4 stage. A second pulse of Pmir-1::GFP-pest expression completely overlapped with the L4 lethargus period and continued into early adulthood. Collectively, these results suggest that the expression patterns of lin-4, let-7 and mir-1 are dynamic throughout development and that the cyclical transcription of these miRNAs is mediated by their cognate promoter sequences.
• Primary Identifier: Expr12251

0 Anatomy Terms

3 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002285	let-7	C05G5.6	Caenorhabditis elegans
WBGene00002993	lin-4	F59G1.6	Caenorhabditis elegans
WBGene00003260	mir-1	T09B4.11	Caenorhabditis elegans

0 Life Stages