1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002993 | lin-4 | F59G1.6 | Caenorhabditis elegans |
| Pattern | A lin-4::YFP reporter gene is robustly expressed in all somatic cells of the L1 within 12 hr of hatching in the presence of food, but it is not expressed during L1 arrest. | Primary Identifier | Expr3869 |
| Remark | Gene_regulation: Because the lin-4::YFP reporter gene contains only genomic regulatory sequence fused to the YFP coding sequence, this result demonstrates that lin-4 transcription is repressed by starvation and/or activated by nutrition, as opposed to a posttranscriptional regulatory mechanism. Consistent with other aspects of the L1-arrest-defective phenotype of daf-16(mgDf50), lin-4::YFP is expressed in starved daf-16(mgDf50) L1s. However, the penetrance of lin-4::YFP expression is relatively low, visible in only 3% and 8% of larvae after 3 and 7 days of starvation, respectively [0% in wild-type and hsf-1(sy441) L1s]. It is intriguing to speculate that nutritional control of developmental timing could be mediated by direct transcriptional regulation of lin-4 by DAF-16. However, given the low penetrance of the lin-4::YFP-expression phenotype together with the presumption that DAF-16 function likely results in global repression of gene expression during starvation, the simplest interpretation is that DAF-16 represses lin-4 transcription indirectly. Thus, the low penetrance of the lin-4::YFP-expression phenotype in starved daf-16(mgDf50) L1s likely reflects the proportion of individuals that develop to the relatively late period in the L1 stage when lin-4::YFP expression becomes visible. Alternatively, DAF-16 could regulate lin-4 directly, but initial expression levels could be too low for detection by reporter gene. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00002993 | lin-4 | F59G1.6 | Caenorhabditis elegans |
