WormMine: ExpressionPattern Expr13459

• Primary Identifier: Expr13459
• Subcellular Localization: To explore the relative cortical localization patterns of LIN-5 and dynein, egfp::lin-5; mcherry::dhc-1 embryos were imaged by SDCM. In late metaphase, eGFP::LIN-5 became modestly enriched at the cortex as compared with the adjacent cytoplasm, both in the anterior and posterior. Although this was not observed for dynein, both LIN-5 and dynein showed higher cytoplasmic levels in the anterior compared with the posterior during all mitotic stages. This gradient affected the relative cortex/cytoplasm ratios calculated for the anterior and posterior and caused the increase of cortical levels observed in the posterior, which was not observed when a mean cytoplasmic value was used for normalization of all points instead.During anaphase, cortical enrichment of eGFP::LIN-5 increased substantially, most noticeably in the posterior. When averaging fluorescence intensities from multiple embryos, mCherry::DHC-1 did not show clear enrichment at the cortex above local cytoplasmic levels during anaphase. This reflects the patchy appearance of cortical dynein enrichment along the A-P axis. Importantly, side-by- side comparison of nonnormalized cortical line scans revealed a strong cross-correlation between cortical eGFP::LIN-5 and mCherry::DHC-1. This indicates that both proteins follow a similar cortical distribution, in agreement with LIN-5-dependent dynein localization. These protein localization patterns can account for force asymmetries in anaphase, but not during metaphase spindle displacement.

0 Anatomy Terms

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002994	lin-5	T09A5.10	Caenorhabditis elegans

0 Life Stages