| Primary Identifier | Expr3521 | Remark | To determine whether the antibody was against the Gal-T2 protein and not the recombinant tags or putative N- or O-glycan side chains, Western blot analysis against recombinant and deglycosylated Gal-T2 protein preparations were used. ELISA and Western blot analysis showed that the antibody recognized a recombinant Gal-T2 (31 to 330-aa) protein preparation, lacking the purification tags. |
| Subcellular Localization | The anti-Gal-T2 scFv showed a strong immunofluorescence signal in all cells of early and late embryo specimens, revealing numerous punctate organelles surrounding the nucleus. Nuclei were always devoid of fluorescence and the pattern of anti-Gal-T2 scFv antibody fluorescence is similar to that seen with other examples of worm Golgi proteins. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00005020 | sqv-2 | Y110A2AL.14 | Caenorhabditis elegans |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. | embryo Ce | WBls:0000003 |
