WormMine: LifeStage WBls:0000686

• Definition: The fourth sub-stage of the L4 larva. vulFs have separated and the apex of the lumen is flat and capped by the anchor cell. The uterine lumen starts to form. At 20 Centigrade: 38.1 hours after hatching, 4.1 hours from the L3-to-L4 molt.
• Primary Identifier: WBls:0000686
• Public Name: L4.3 larva Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	A C. elegans life stage that occurs during the L4 larval life stage		C. elegans life stage occurring during L4 larval stage	WBls:0000821

0 Expression Clusters

10 Expression Patterns

• Primary Identifier: Expr16423
• Pattern: We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.

• Primary Identifier: Expr16424
• Pattern: We found a similar pulse of NHR-23::mScarlet in L4.3 vulval precursor cells as that reported by Kinney et al.. nhr-23::mScarlet was also detectable in seam and hypodermal cells at this stage, epithelial cells that synthesize cuticular components. NHR-23::mScarlet was detected in pachytene nuclei in L4 animals and its zone of expression became restricted in young adult animals. This expression pattern is again similar to NHR-23::GFP::AID*::3xFLAG (Ragle et al. 2020). However, we also observed some interesting differences compared to NHR-23::GFP::AID*::3xFLAG. In our previous work, we observed that NHR::23::GFP became undetectable in ovulating adults (Ragle et al. 2020). In contrast, NHR-23::mScarlet appears to be diffusely expressed in young adult residual bodies and spermatocytes. This diffuse expression persists into ovulating adults, and appears restricted to the spermatheca. The NHR-23::mScarlet signal is specific to nhr-23::mScarlet::3xMyc animals as no diffuse expression in the germline is observed in wild-type animals. One can observe the diffuse expression pattern in L4.5 germlines several rows of cells after NHR-23::mScarlet::3xMyc localization is lost from nuclei.

• Primary Identifier: Expr12298

• Primary Identifier: Expr12304

• Primary Identifier: Expr12305
• Pattern: Only reporter in this study where expression was obviously turned off during L4.

• Primary Identifier: Expr15947
• Subcellular Localization: The transmembrane eLRRon protein LET-4 marked all vulval apical membranes during the late L4.2-L4.7 period, and thereafter appeared intracellular.

• Primary Identifier: Expr12301
• Pattern: Expression in E variable in L4.3and L4.4, consistent in later substages; expression in F variable throughout.

• Primary Identifier: Expr12300
• Pattern: Variable expression in all substages; low level of expression in B2, C from L4.1 to L4.7.

• Primary Identifier: Expr12299
• Pattern: Variable expression in L4.1; consistent in later sub-stages.

• Primary Identifier: Expr12306
• Pattern: Variable expression possibly due to loss of extrachromosomal array.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fifth sub-stage of the L4 larva. utse is visible as a thin layer separating the vulval and uterine lumens. 'Fingers' are formed at the sides of the vulva next to vulB1 and vulB2. The uterine lumen is fully distended. At 20 Centigrade: 39.0 hours after hatching, 5.0 hours from the L3-to-L4 molt.		L4.4 larva Ce	WBls:0000687

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third sub-stage of the L4 larva. All vulval cells complete migration. vulA, vulC, and vulD undergo cell fusion. The vulval lumen has widened and a prominent kink has formed between vulC and vulD. The uterine lumen has not started to form. At 20 Centigrade: 36.8 hours after hatching, 2.8 hours from the L3-to-L4 molt.		L4.2 larva Ce	WBls:0000685

0 Sub Stages