WormMine: LifeStage WBls:0000028

• Definition: The stage during L2-L3 molt when an animal becomes lethargic, ceases pharyngeal pumping and synthesizes new cuticle under the old.
• Primary Identifier: WBls:0000028
• Public Name: L2-L3 lethargus Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage when an animal shifts from L2 larva to L3 larva. It includes the synthesis of new cuticle, cease of phrayngeal pumping during a lethargus stage, and the shed off of old cuticle.		L2-L3 molt Ce	WBls:0000029
	C. elegans life stage that occurs during a larval molt		C. elegans life stage occurring during larval molt	WBls:0000824

0 Expression Clusters

1 Expression Patterns

• Primary Identifier: Expr12872
• Pattern: Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977). A lin-46 translational fusion reporter showed a similar expression pattern, although it was less intense. The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus. Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977).
• Subcellular Localization: The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage during L2-L3 molt, after the brief lethargus period, when an animal sheds off old cuticle.		L2-L3 ecdysis Ce	WBls:0000047

0 Preceded By

0 Sub Stages