WormMine: LifeStage WBls:0000074

• Definition: At 20 Centigrade: 11-15 days after L4-adult molt. 14-18 days after first cleavage.
• Primary Identifier: WBls:0000074
• Public Name: 11-15 days post-L4 adult hermaphrodite Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057
	A C. elegans life stage that occurs during the adult life stage		C. elegans life stage occurring during adulthood	WBls:0000816

6 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Proteins that showed significantly decreased expression in day 10 heat shocked adults comparing to heat shocked young adult animals.	N.A.	WBPaper00044746:aging_downregulated_HeatShock
	Proteins that showed significantly increased expression in day 10 heat shocked adults comparing to heat shocked young adult animals.	N.A.	WBPaper00044746:aging_upregulated_HeatShock
	Proteins that showed significantly increased expression in day 10 adults comparing to young adults.	N.A.	WBPaper00044746:aging_upregulated_Day10
	Proteins that were rapidly synthesized between day 11 and day 16 adults.	The mass tolerance was set at 10ppm for precursor ions and 0.8Da for product ions. Strict Lys-C specificity was applied, and missed cleavages were not allowed. BuildSummary was used to generate a confident protein list with a peptide false discovery rate <= 0.01. Other criteria for significant peptide identifications included the following: peptides must be composed of at least six amino acid residues and have a minimum Mascot score of 20, which corresponds to Mascot absolute probability of 0.01.	WBPaper00046446:rapid-synthesized_AgingAdult
	Proteins that showed significantly decreased expression in day 10 adults comparing to young adults.	N.A.	WBPaper00044746:aging_downregulated_Day10
	Genes with dynamic regulation pattern in signaling pathways during aging, based on re-analysis of microarray datasets.	The approaches of Gene Set Enrichment Analysis (GSEA) were performed using Category package in version 2.6.0 of Bioconductor to identify the significantly enriched pathways and genes in both above two subsets. In this analysis, the gene sets with less than 10 genes were excluded. The t-statistic mean of the genes was computed in each KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway. Using a permutation test with 1000 times, the cutoff of significance level p value was set as 0.01 for the most significant pathways related to aging. Accordingly, the significant pathways and genes were then identified under the comparison between different time points, including from L4 to D6 and from D6 to D15.	WBPaper00045140:aging_dynamic_regulation

2 Expression Patterns

• Primary Identifier: Expr12740
• Pattern: The UNC-104::mCherry signal is specifically decreased in the DNC, but not VNC, of day 12 and day 18 animals compared to that of day 1, indicative of compromised UNC-104 activity and kinesin motor's failure to travel to distal axons with age.

• Primary Identifier: Expr11969
• Pattern: mir-228::GFP expression increases during early adulthood, reaches a peak, and then begins to slowly decrease in mid to late adulthood.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	At 20 Centigrade: 16-18 days after L4-adult molt. 18-21 days after first cleavage.		16-18 days post-L4 adult hermaphrodite Ce	WBls:0000111

2 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	At 20 Centigrade: 10-11 days after L4-adult molt. 13-14 days after first cleavage.		10-days post-L4 adult hermaphrodite Ce	WBls:0000674
	At 20 Centigrade: 7-10 days after L4-adult molt. 10-13 days after first cleavage.		7-10 days post-L4 adult hermaphrodite Ce	WBls:0000070

0 Sub Stages