1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00008919 | vps-36 | F17C11.8 | Caenorhabditis elegans |
| Primary Identifier | Expr12779 | Subcellular Localization | ESCRT-II components localize at the sarcoplasmic reticulum (SR) attachment zones. VPS-36, VPS-22 and VPS-25 in the I band and at the interface between the I and A bands. VPS-36::GFP presents a dual localization in the sarcomere. The majority of the signal largely co-localizes with ATN-1/alpha-actinin or DEB-1/vinculin suggesting it is strongly associated with dense bodies (DB).However, VPS-36::GFP is also present in small patches localized between the alignment of DB, probably apposed to the sarcolemma. Correlative light-electron microscopy (CLEM) on BWM confirms that VPS-36 is associated with both DB and an electron dense structure localized between the plasma membrane and the lattice of contractile filaments. This pattern corresponds to the organization of the SR network within BWM, which forms thin tubular structures along the dense bodies and the plasma membrane (Hammarlund et al., 2000). A similar localization to VPS-36 is also detected for ESCRT-II subunits VPS-25::GFP and VPS- 22::GFP. The localization of endogenous ESCRT-II was further confirmed using two antibodies directed against VPS-36 (this study) and the whole ESCRT-II complex (Schuh et al., 2015), respectively. Moreover, the colocalization analysis with the ryanodine receptor UNC-68, involved in SR calcium release, indicates that ESCRT-II localizes to the sarcoplasmic reticulum. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00008919 | vps-36 | F17C11.8 | Caenorhabditis elegans |
