WormMine: ExpressionPattern Expr2985

• Pattern: Expression of the pha-1 reporter was first detected at the approximately 100-cell stage in all somatic cells of the embryo. This pattern of widespread expression continued through later stages of embryonic development, diminishing somewhat in intensity by the time of hatching. Expression was also observed in early-larval-stage animals and was present but reduced in late-stage larvae and adults.
• Primary Identifier: Expr2985
• Remark: Similar expression patterns were also observed using reporter constructs containing approximately 2.9 kb of upstream regulatory sequences together with genomic sequences encoding the first 137 aa of PHA-1 fused to a GFP containing a nuclear localization signal.
• Subcellular Localization: Most if not all PHA-1::GFP fusion protein localized to the cytoplasm. This was particularly apparent in larvae, where the contribution of nonspecific GFP fluorescence coming from layers outside of the central focal plane was minimized. No evidence was observed for any dynamic regulation of PHA-1 with respect to its subcellular localization. Although these findings do not rule out the possibility that some PHA-1 protein may normally be present in nuclei, they strongly suggest that PHA-1 resides primarily, if not exclusively, in the cytoplasmic compartment. Moreover, although it was possible that the tagged fusion protein may have been mislocalized, either due to the presence of the C-terminal GFP extension, or simply through overexpression, the ability of the fusion protein to substitute functionally for wild-type PHA-1 suggests that it is localizing to the correct cellular compartment.

0 Anatomy Terms

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004010	pha-1	Y48A6C.5	Caenorhabditis elegans

9 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010