WormMine: ExpressionPattern Expr1162

• Pattern: POD-1 localization in P cells is cell cycle dependent. In the earliest one-cell embryos before pronuclear meeting (P0), cortical POD-1 is either anteriorly enriched (12/24 embryos), posteriorly enriched (5/24 embryos), or uniformly localized (7/24 embryos). From pronuclear meeting to metaphase, cortical POD-1 is almost always anteriorly enriched (30/39 embryos). At anaphase, POD-1 begins to transition from asymmetric to symmetric localization, such that by telophase cortical POD-1 can be found at equivalent levels in the anterior and posterior cortices (10/11 embryos). In addition to cell cortices, POD-1 antibodies stain punctate cytoplasmic structures. POD-1 can be found in the posterior-most cortex, albeit at lower levels than the anterior. The localization of POD-1 in the germ-line precursors of the two-cell and four-cell embryo is also cell cycle dependent such that early in the cell cycle, POD-1 is not asymmetric along the a-p axis, but becomes anteriorly enriched leading up to metaphase.
• Primary Identifier: Expr1162
• Remark: To confirm that the localization patterns described are specific to POD-1, pod-1(ye11) mutant embryos were stained with both POD-1 antibodies. All cortical and cytoplasmic POD-1 staining was eliminated in the mutant embryos.
• Subcellular Localization: punctate cytoplasmic structures

0 Anatomy Terms

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004075	pod-1	Y76A2B.1	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 0-20min after first cleavage at 20 Centigrade. Contains 2 cells.		2-cell embryo Ce	WBls:0000007
	Also called zygote. -50min till 0min of first cleavage at 20 Centigrade. Contains 1 cell.		1-cell embryo Ce	WBls:0000006
	The C. elegans life stage spanning 20-40min after first cleavage at 20 Centigrade. Contains 4 cells.		4-cell embryo Ce	WBls:0000008