WormMine: LifeStage WBls:0000048

• Definition: The stage during L2d-dauer molt, after the brief lethargus period, when an animal sheds off old cuticle.
• Primary Identifier: WBls:0000048
• Public Name: L2d-dauer ecdysis Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage when an animal shifts from L2d larva to dauer larva. It includes the synthesis of new cuticle, cease of pharyngeal pumping during a lethargus stage, and the shed off of old cuticle.		L2d-dauer molt Ce	WBls:0000031
	C. elegans life stage that occurs during a larval molt		C. elegans life stage occurring during larval molt	WBls:0000824

0 Expression Clusters

1 Expression Patterns

• Primary Identifier: Expr16428
• Pattern: Transgenic worms expressing mCherry under the daf-42 promoter showed expression in seam cells between 48 and 60 HAE, which corresponds to the time of appearance of the dead L2d phenotype. At 60 HAE, we observed expression in the seam cell, hypodermis, and at the surface of the worm. At 72 HAE, ecdysis had begun, and some worms had detached their head or tail from the old L2d cuticle, and the DAF-42::GFP signal did not remain in the L2d cuticle but was localized with the dauer worm inside the L2d cuticle. Together, our results show that daf-42 is expressed during the L2d-to-dauer transition in seam cells and is secreted toward the surface of the dauer worm, such as the hypodermis or cuticle.

0 Followed By

0 Preceded By

0 Sub Stages