WormMine: AnatomyTerm WBbt:0005753

• Definition: a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail
• Name: seam cell
• Primary Identifier: WBbt:0005753
• Synonym: lateral hypodermis

26 Children

Definition	Name	Synonym	Primary Identifier
Seam hypodermal cell, postembryonic blast cell	H1L	lineage name: ABplaaappp	WBbt:0004766
Seam hypodermal cell, postembryonic blast cell	H1R	lineage name: ABarpapppp	WBbt:0004765
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2L	lineage name: ABarppaaap	WBbt:0004764
Seam hyopdermal cell, postemb. blast cell, L1 deirid socket	H2R	lineage name: ABarpppaap	WBbt:0004763
Seam hypodermal cell	H0L	lineage name: ABplaaappa	WBbt:0004778
Seam hypodermal cell	H0R	lineage name: ABarpapppa	WBbt:0004777
Tail seam hypodermal cell, postembryonic blast cell, functions as phasmid socket in L1	TL	lineage name: ABplappppp	WBbt:0004946
Tail seam hypodermal cell, postembryonic blast cell, functions as phasmid socket in L1	TR	lineage name: ABprappppp	WBbt:0004944
seam cell in male tail; does not fuse with main seam, does not make alae.	male tail seam cell	male posterior seam	WBbt:0006912
Seam hypodermal cell, postembryonic blast cell	V4L	lineage name: ABarppappa	WBbt:0004894
Seam hypodermal cell, postembryonic blast cell	V4R	lineage name: ABarpppppa	WBbt:0004892
Seam hypodermal cell, postembryonic blast cell	V5L	lineage name: ABplapapaap	WBbt:0004890
Seam hypodermal cell, postembryonic blast cell	V5R	lineage name: ABprapapaap	WBbt:0004876
Seam hypodermal cell, postembryonic blast cell	V2L	lineage name: ABarppapap	WBbt:0004902
Seam hypodermal cell, postembryonic blast cell	V2R	lineage name: ABarppppap	WBbt:0004900
Seam hypodermal cell, postembryonic blast cell	V3L	lineage name: ABplappapa	WBbt:0004898
Seam hypodermal cell, postembryonic blast cell	V3R	lineage name: ABprappapa	WBbt:0004896
Seam hypodermal cell, postembryonic blast cell	V1R	lineage name: ABarppppaa	WBbt:0004904
Seam hypodermal cell, postembryonic blast cell	V1L	lineage name: ABarppapaa	WBbt:0004905
Seam hypodermal cell, postembryonic blast cell	V6L	lineage name: ABarppappp	WBbt:0004875
Seam hypodermal cell, postembryonic blast cell	V6R	lineage name: ABarpppppp	WBbt:0004874
seam syncytium on the left flank of an adult animal.	adult seam cell left		WBbt:0006809
seam syncytium on the right flank of an adult animal.	adult seam cell right		WBbt:0006810
post-embryonic seam cells that make alae, hermaphrodite.	hermaphrodite alae seam cell	se_herm	WBbt:0006913
post-embryonic seam cells that make alae in the male.	se_male	male alae seam cell	WBbt:0006914
Postembryonic seam hypodermal cells, make alae, found in both male and hermaphrodite.	se seam cell	se	WBbt:0008156

8 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:seam_expressed
	Single-cell RNA-Seq cell group 0_0 expressed in seam.	scVI 0.6.0	WBPaper00065841:0_0
	Single-cell RNA-Seq cell group 34 expressed in: Seam cells (grd+).	CellRanger, DecontX, Monocle3, Louvain algorithm.	WBPaper00065623:34
	Top 300 transcripts enriched in seam cell according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:Seam_cell
	Transcripts uniquely expressed in seam cells, according to PAT-Seq analysis using Pgrd-10-GFP-3XFLAG mRNA tagging.	Cufflinks FPKM value >=1.	WBPaper00050990:seam_enriched
	Single-cell RNA-Seq cell group 82_1 with unidentified tissue expression pattern.	scVI 0.6.0	WBPaper00065841:82_1
	Single-cell RNA-Seq cell group 82_0 with unidentified tissue expression pattern.	scVI 0.6.0	WBPaper00065841:82_0
	Single-cell RNA-Seq cell group 32 expressed in: Seam cells (bus+) and excretory duct subpopulation.	CellRanger, DecontX, Monocle3, Louvain algorithm.	WBPaper00065623:32

733 Expression Patterns

• Primary Identifier: Expr4832
• Remark: Picture: Figure 1B III and IV.
• Pattern: The promoter extracted from C15C7.1 directs GFP expression in the seam cells).

• Primary Identifier: Expr4836
• Remark: Picture: Figs. 4A-D.
• Pattern: In hermaphrodites, the expression of bro-1 was restricted to seam cells. Its expression was first detected at bean-stage embryos and persisted throughout the developmental stages. In the male tail, bro-1 was also expressed in the ray precursor cells.
• Subcellular Localization: GFP::BRO-1 was localized to both the cytoplasm and the nucleus.

• Primary Identifier: Expr4824
• Remark: Picture: Fig. 10 I.
• Pattern: The hex-4::gfp line displayed staining of seam cells in late embryos and L1 larvae. Expression was restricted to the embryonal and L1 stages.

• Primary Identifier: Expr4680
• Subcellular Localization: At 10 to 20 min after hatch, nuclear expression of CDC-6 was first observed in the seam cells, and by 100 to 115 min after hatch, nuclear levels were high. As cells entered S phase, nuclear levels of CDC-6 dropped gradually with a concomitant increase in cytoplasmic CDC-6. Beyond 180 min after hatch, endogenous CDC-6 remained at high levels in the cytoplasm, although residual nuclear CDC-6 was still present.

• Primary Identifier: Expr4764
• Pattern: CO3H5.2 gene has a broad expression pattern. This includes expression in the pharynx and pharyngeal gland cells, seam cells, spermatheca, stomatointestinal muscle, vulva and body wall muscle.

• Primary Identifier: Expr4980
• Remark: Picture: Fig 4B. Similar expression patterns were observed for a variety of different transgenic alleles, carrying both extrachromosomal and integrated arrays.
• Pattern: In all larval stages, BRO-1::GFP is expressed in H0 to 2, V1 to 6 and T seam progeny. Faint expression is also observed in hypodermal nuclei, some of which are embryonically derived and which are not therefore simply anterior daughters of the L1 stem cell division containing a perdurance of GFP expression. Expression of BRO-1::GFP was also seen in the uterine seam (utse) during late L4 in hermaphrodites.
• Subcellular Localization: BRO-1::GFP is localised to both the cytoplasm and nucleus.

• Primary Identifier: Expr4982
• Remark: Picture: Fig. 4D, 4E.
• Pattern: BRO-1 and RNT-1 are co-expressed in seam and muscle cells.
• Subcellular Localization: Co-localisation is shown using rescuing bro-1::DsRed (pAW303) and rnt-1::GFP (pAW260) constructs. Faint BRO-1::RFP and RNT-1::GFP co-localisation is also observed in certain body wall muscle cells.

• Primary Identifier: Expr4943
• Pattern: strong 3-fold emb bwm that fades and becomes mosaic in late L's and adults, vul, anal dep, spinchter, mu int; seam cells in one line at least

• Primary Identifier: Expr4901
• Remark: Data came from J Shim and J Lee (submitted), cannot locate original publication.
• Pattern: bro-1 is expressed only in the hypodermal seam cells. It is expressed throughout development.
• Subcellular Localization: The nematode bro-1 is mostly localized in the cytoplasm but is also present in the nucleus.

• Primary Identifier: Expr4395
• Pattern: The pps-1p::EGFP reporter is widely but tissue-specifically expressed in somatic cells. pps-1p::EGFP is strongly expressed in seam cells, gland cells, and neuronal support cells (amphid sheath cells) throughout development. Relatively weak expression was also detected in the hypodermis and the phasmid support cells during larval development. Additionally, weak expression was observed in the intestine at the adult stage. No signal was found in the neurons and muscles.

• Primary Identifier: Expr4396
• Pattern: The tissues expressing pps-1(FL)::EGFP and the timing of its expression were almost identical to those expressing pps-1p::EGFP. See Expr4395
• Subcellular Localization: pps-1(FL)::EGFP is dominantly localized in nuclei of all expressing cells.

• Primary Identifier: Expr4383
• Pattern: The eps-8p::nls::gfp reporter is expressed in a variety of cell types including neurons, gut, muscle and seam cells as well as in the VPCs and their descendants. A time course analysis revealed a dynamic expression pattern of eps-8::nls:.gfp in the vulval cells. In mid-L2 larvae, eps-8p::nls::gfp is weakly expressed in all VPCs except for P3.p. Around 6 h later in early L3 larvae, before the first round of vulval cell divisions, expression is strongest in P6.p, lowest in P5.p and P7.p and intermediate in P3.p, P4.p and P8.p.

• Primary Identifier: Expr4327
• Remark: No obvious difference in expression was observed between this particular mir-84::gfp fusion gene and one in which 8.1 kb of sequence upstream of mir-84 was fused to yellow fluorescent protein, kindly provided by A. Yoo and I. Greenwald.
• Pattern: Transgenic mgEx674[mir-84::gfp] animals expressed GFP in the lateral hypodermal seam cells and other cells. The mir-84::gfp reporter was expressed in seam cells during early larval stages in some transgenic animals, although expression was more prevalent in L3-stage and older animals.

• Primary Identifier: Expr4435
• Pattern: Expressed in the seam cells from embryo to L4.

• Primary Identifier: Expr4437
• Pattern: Expressed in the seam cells from L1 to adult. Expressed in the rectal epithelial cells from L1 and maintain through adulthood. Expressed in the pro- and metacorpus and isthmus.

• Primary Identifier: Expr4439
• Pattern: Expressed in the seam cells at embryo. Expressed in socket cells of IL or OLQ. Expression shown in the intestine, usually stronger in the cells at the terminal regions.

• Primary Identifier: Expr4431
• Pattern: Expressed in the seam cells at adult stage. Expressed in the rectal epithelial cells only in the fourth larval stage (L4). Expressed in VulB and VulD. Expressed in anterior arcade cells. Expressed in a pharyngeal neuron in the metacorpus extending a neurite anteriorly and an axon posteriorly.

• Primary Identifier: Expr4433
• Pattern: Expressed in the seam cells from L1 to L4.

• Primary Identifier: Expr4271
• Pattern: Anti-MIG-22 stained DTCs and the proximal arms of the gonad in a punctate pattern. Strong signals were detected in oocytes in adult gonads. MIG-22 was also detected in the developing vulval epithelium and seam cells. All these signals were significantly lower in mig-22(tk24) animals.

• Primary Identifier: Expr4249
• Pattern: The NUD-2 fusion protein was expressed within neurons of the ventral cord as well as the pharynx, seam cells of the hypodermis, and in the vulva muscle cells.

• Primary Identifier: Expr4201
• Pattern: This construct expressed GFP ubiquitously in early embryos. The expression became progressively more restricted in older embryos and young larvae, and was not observed in adults. In larvae, expression was observed in dividing cells: ventral nerve cord neuroblasts, vulval precursors, dividing hypodermal seam cells, and the Q neuroblasts and their descendants.

• Primary Identifier: Expr4654
• Remark: Since utse aff-1 expression and AC-utse fusion occur almost simultaneously, it is possible that aff-1 expression detected in the utse is actually a contribution from the AC cytoplasm after the fusion event. To test this, authors examined utse aff-1 expression in lin-29(n482) mutant worms where AC-utse fusion does not occur. aff-1 expression in the utse in these mutants indicated that aff-1 is specifically expressed in the utse cells and is not a consequence of AC to utse cytoplasmic GFP diffusion after fusion.
• Pattern: Specific and continuous expression was detected in the AC from the invasion of the vulval primordium at mid-L3 until its fusion with the utse cells. As the vulva completes its invagination in the L4, the utse syncytium starts to express aff-1, resulting in coexpression of aff-1 in both cells prior to their fusion. Thus, AFF-1 is dynamically expressed in a specific group of cells that undergo cell fusion during normal development. aff-1 expression is first detected in the embryonic hyp5 cell and later during larval development in various cell types, including pharyngeal muscles (Pm3 and Pm5), uterine rings (Ut2 and Ut4), head and tail neurons, sheath cells of chemosensory neurons, and male tail neurons). aff-1 is also expressed in vulval vulD and the seam cells shortly before these cells fuse. In general, the myoepithelial cells of the pharynx and the epithelial cells in the uterus, vulva, and hypodermis that express aff-1 undergo fusion. Thus, AFF-1 is dynamically expressed in a specific group of cells that undergo cell fusion during normal development.

• Primary Identifier: Expr4655
• Pattern: AFF-1::GFP was weakly expressed on the plasma membrane and in intracellular organelles in hyp5, seam cells, vulD vulval precursors, and AC before, during, and after cell-cell fusion.
• Subcellular Localization: Expressed on the plasma membrane and in intracellular organelles.

• Primary Identifier: Expr4605
• Remark: Reporter gene fusion type not specified.
• Pattern: The GFP construct was broadly expressed throughout the animal with fluorescence strongest in the seam cells, body wall and pharyngeal muscle, vulval cells, spermatheca, the tail, and many neurons.

• Primary Identifier: Expr4483
• Remark: No detailed description on expression pattern in other life stage.
• Pattern: Expressed in lateral hypodermal cells from L2 to L4, vulval muscle in some adult animals, anterior neurons (not individually identified in this study) from L2 to L4, posterior neurons in some L2/L3 and adult animals, body wall muscle from L2 to adult.

• Primary Identifier: Expr4470
• Remark: No detailed description on expression pattern in other life stage.
• Pattern: Expressed in main body hypodermis at L2/L3, L4 and adult, ventral nerve cord at L2/L3, L4 and adult, lateral hypodermal cells at L2/L3, L4 and adult, vulval muscle at adult stage, anterior neurons (not individually identified in this study) from L2 to adult.

• Primary Identifier: Expr4467
• Remark: No detailed description on expression pattern in other life stage.
• Pattern: Expressed in main body hypodermis at L2/L3, ventral nerve cord at L4, lateral hypodermal cells at L2/L3, anterior neurons (not individually identified in this study) at L2/L3, L4 and adult, pharynx at L2/L3, L4 and adult, cells adjacent to posterior of gut around rectum during L4 and adult stage.

• Primary Identifier: Expr4469
• Remark: No detailed description on expression pattern in other life stage.
• Pattern: Expressed in main body hypodermis at L2/L3 and L4, ventral nerve cord at L2/L3, L4 and adult, lateral hypodermal cells at L2/L3 and adult, vulval muscle at adult stage, anterior neurons (not individually identified in this study) from L2 to adult.

• Primary Identifier: Expr4462
• Pattern: Expressed in the seam cells. Expression decrease in old adults. Expressed in anterior and Posterior arcades.

• Primary Identifier: Expr4450
• Pattern: Expressed in the seam cells from embryo to L1. Expressed in the rectal epithelial cells from L1 and maintain through adulthood. Expressed in VulA and VulB.

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	A third stage larva specialized for dispersal and long term survival.		dauer larva Ce	WBls:0000032

1 Parents

Definition	Name	Synonym	Primary Identifier
primary cell type that forms the hypodermis.	hypodermal cell		WBbt:0007846