WormMine: AnatomyTerm WBbt:0004643

• Definition: Ventral cord motor neuron, innervates ventral body muscles, also interneuron in ring
• Name: VB1
• Primary Identifier: WBbt:0004643
• Synonym: lineage name: P1.aaap

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree P1.aaap	P1.aaap nucleus		WBbt:0003218

5 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts enriched in VB1 according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00066550:VB1_enriched
	Single-cell RNA-Seq cell group 6_1 expressed in neuron.	scVI 0.6.0	WBPaper00065841:6_1
	Single-cell RNA-Seq cell group 6_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:6_0
	Transcripts enriched in VB according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:VB_enriched
	Transcripts enriched in VB01 according to single cell RNAseq.	Genes that pass the Bonferroni threshold for multiple comparisons (q < 0.05) are significantly enriched.	WBPaper00061651:VB01_enriched

37 Expression Patterns

• Primary Identifier: Expr4784
• Pattern: Three independent transgenic lines display GFP fluorescence exclusively in VB motor neurons in the ventral nerve cord. ceh-12::GFP, is expressed in all 11 VB motor neurons and in a single head neuron, RID; occasional weak GFP expression was observed in the pharyngeal/intestinal valve cell and in the excretory gland cell.

• Primary Identifier: Expr15649

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr15570

• Primary Identifier: Expr1872
• Pattern: In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap euroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. In late L2 larvae anti-PAG-3 staining was seen in approximately two dozen cells in the head, all six mechanosensory neurons, the BDU neurons, approximately ten cells in the tail as well as in the ventral cord. PAG-3 staining of many cells in the head and tail remained detectable in adult animals. In the ventral cord, PAG-3 was first detected in the Pn.aa neuroblasts. PAG-3 was not detected in the Pn.ap neuroblasts or their descendants. PAG-3 was present equally in each of the descendant cells of Pn.aa after subsequent rounds of division (i.e. the Pn.aaa, Pn.aap, Pn.aaaa and Pn.aaap cells), including the three differentiating neurons generated by each Pn.aa neuroblast. In most cells, PAG-3 protein became undetectable shortly after the cells had been generated in the L1, but PAG-3 was present in six cells in the ventral cords of adults. PAG-3 expression persisted throughout the life of the animal in four cells in the retrovesicular ganglion at the anterior end of the ventral cord and in two cells in the posterior ventral cord. In newly hatched L1-stage larvae, before the initiation of the postembryonic W and P cell lineages, two cells in the retrovesicular ganglion expressed PAG-3. Based on position, these cells were most likely the RIG interneurons. After completion of the W and P cell lineages, two additional cells in the retrovesicular ganglion and two cells in the posterior ventral cord contained detectable PAG-3 protein. These nuclei might be the two AVF and the VA11 and VA12 neurons, respectively. This hypothesis was confirmed by staining animals carrying an integrated Punc-4lacZ reporter, which is expressed in the AVF and VA as well as other neurons, with PAG-3 antiserum and monoclonal antibody against beta-galactosidase. PAG-3 protein was expressed more widely in the nervous system than had been observed using the Ppag-3lacZ reporter. PAG-3 was detected during embryonic development in many nuclei ~280 minutes after fertilization.

• Primary Identifier: Expr521
• Remark: Transgenic Marker: rol-6(su1006).
• Pattern: Expression in touch-sensitive neurons, motor neurons, and interneurons which starts in the 2-fold embryo and disappears by L3. Sporadic expression seen in retrovesicular ganglion at L1. Expressed in ALM, PLM and BDU cells at 2-fold embryo, L1 and L2. In AVM, PVM cells at L1 and L2. Expressed in ventral cord motor neurons VA2-VA12, VB1-VB11, AVF cells, and precursors at L1 and L2.

• Primary Identifier: Expr15644

• Primary Identifier: Expr15646

• Primary Identifier: Expr12716

• Primary Identifier: Expr12717

• Primary Identifier: Expr15315

• Primary Identifier: Expr15316
• Pattern: acr-5 ORF

• Primary Identifier: Expr15633

• Primary Identifier: Expr12715

• Primary Identifier: Expr15616

0 Life Stages

4 Parents

Definition	Name	Synonym	Primary Identifier
Neuron class of eleven motoneurons, distributed along the ventral cord, which innervate ventral body muscles.	VB neuron		WBbt:0005336
neuron whose cell body is in the retrovesicular ganglion.	retrovesicular ganglion neuron	retrovesicular ganglion	WBbt:0005403
neuron that has neural processes in the nerve ring.	nerve ring neuron		WBbt:0006974
	P1.aaa		WBbt:0008511