|
|
|
Expr4775
|
Expressed in I1 neurons. |
|
|
|
|
Expr15590
|
|
|
|
|
|
Expr15558
|
|
|
|
The cellular expression pattern for nlp-3 fragments was indistinguishable from the cellular expression pattern for nlp-3 subcloned constructs. Transgenic lines were generated by coinjection of the nlp::gfp construct (5070 ng/ul) and lin-15 rescue construct (pJM24,100 ng/ul) into lin-15(n765ts) animals. At least two independent transgenic lines were analyzed for each nlp gene; 5-10 animals were scored per line. 1,1'-Dioctadecyl-3,3,3',3 -tetramethylindodicarbocyanine perchlorate (Molecular Probes) was used to stain amphid and phasmid neurons to facilitate identification. |
|
Expr1688
|
Expressed in ADF, ASE, ASH, AWB, ASJ, BAG, HSN, I1, I2, I3, I4, MI, M3, NSMR, 3 head neurons, VNC, oocytes, I6, M2, pm1VL, intestine. |
|
|
|
|
Expr15571
|
|
|
|
|
|
Expr15572
|
|
|
|
|
|
Expr15573
|
|
|
|
C43H6.9 = glr-7 |
|
Expr822
|
I3, I2, I6, MI, NSM, I1(?). |
|
|
|
|
Expr15579
|
|
|
|
F22A3.3 = glr-8 |
|
Expr823
|
I1, I2, MC, NSM, M3 (left/right), I3, MI M4, M3 (left/right), I6, M5, BDU, ALM, PLM, URB (left/right) |
|
|
|
|
Expr15583
|
|
|
|
|
|
Expr15586
|
|
|
|
|
|
Expr15651
|
|
|
|
|
|
Expr15652
|
|
|
|
|
|
Expr15589
|
|
|
|
|
|
Expr15591
|
|
|
|
|
|
Expr15598
|
|
|
|
|
|
Expr11622
|
Expression of ceh-34::gfp transgene began during embryogenesis. CEH-34::GFP was localized to the nuclei of expressing cells. During embryonic morphogenesis and larval development and throughout adulthood, expression of the ceh-34::gfp transgene was seen predominantly in pharyngeal cells. The ceh-34::gfp transgene was expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2, I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2) and pharyngeal epithelial cells (e1 and e3), and some body wall muscles around the anterior pharynx. |
|
|
|
|
Expr15604
|
|
|
|
|
|
Expr14590
|
Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
|
|
|
|
Expr15608
|
|
|
|
|
|
Expr15611
|
|
|
|
|
|
Expr15436
|
unc-17(3k) |
|
|
Reporter gene fusion type not specified. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). tba-2 in this article is referred as alpha-2 tubulin, while the same authors referred it as tba-2 in later publications such as cgc2176. |
|
Expr597
|
Following hatching, 6 pharyngeal muscle cells stain becoming increasingly intense during larval and adult development. 6-7 neurons are stained in the ventral cord identified as DB motor neurons. Late larval and adult stages, staining of the set of DB and VB motor neurons in the ventral cord and their axonal processes along the ventral cord shows higher intensity of staining in late larval and adult stages. At L4, Pharyngeal muscles and the motor neurons show intense staining in L4 and adult stages. Fusion gene expressed in intestinal nuclei showing a gradient of expression, high staining in the posterior most intestinal nuclei in the tail region to a lower intensity of staining in the anterior intestinal nuclei. Intestinal staining decreases (L3-L4) and adult stages. Staining is detected in entire intestinal organ. Expression in neurons includes a set of DB and VB (19 cells) in the ventral nerve cord, a pair of posterior mechanosensory receptor neuron PLML and PLMR a single interneuron PVT in the pre-anal ganglion and a single neuron ALA in dorsal ganglion in the head. |
|
|
The whole-mount in situ hybridization database by the Kohara Lab [NEXTDB Ver. 3.0 beta (July 07, 2001), yk112c4, shows that the gene is expressed transiently in the midembryonic stage. |
|
Expr2890
|
Expression was detected in some neurons in the head and pharynx of larvae and adult animals. The neurons expressing Ce-TBX-2 was identified in adult animals: they were amphid sensory neurons AWB, AWC, ASJ, and pharyngeal neurons I1, I3, I5, M1, M2, M5, NSM. |
Most Ce-TBX-2 protein molecules were localized in the cytoplasm and not in the nuclei of all the neurons that express this protein. The mutations ut180 and ut192 did not change the localization. |
|
|
|
Expr14024
|
Several head neurons, I1 or I2 pharyngeal neurons, head mesodermal cell?, pharynx, vulva, CAN, VC, DA9, 1 neuron pair in the tail, PVT, another cell in PAG? |
|
|
Picture: Figure 7. |
|
Expr7808
|
A highly reproducible pattern of DKF-1 expression was observed as animals matured from embryo to adult. Intense fluorescence signals corresponding to DKF-1GFP revealed robust kinase accumulation in both (a) a region bounded by the anterior and posterior bulbs of the pharynx and (b) a tail area that contains lumbar, dorsorectal and pre-anal ganglia. Specifically, DKF-1 is differentially enriched in a cluster of cells that are immediately adjacent to the posterior pharyngeal bulb. Strong signals also emanate from cells positioned along the lateral surface of this bulb in animals carrying the dkf-1P::DKF-1GFP transgene. At the anterior pharyngeal bulb, DKF-1 accumulates selectively in bodies and in very thin processes (dendrites and axons) of two neurons. Nearly all cells expressing DKF-1 appear to be neurons. Two fluorescent cells with similar sizes and locations (at the anterior edge of the isthmusposterior bulb) may be M2 motor neurons. The location of the more posterior fluorescent neuron approximates the position of the cell body of an NSM neuron. DKF-1 also accumulates in a cell resembling I1. Other candidate DKF-1-enriched cells in the pharyngeal region include: the AWB, ADL, and ADF chemosensory neurons; and AVB and AIA interneurons.n C. elegans tail, DKF-1GFP expression is differentially elevated in neurons located within the dense neuropile of several tail ganglia. The pattern of fluorescence reveals that cell bodies and/or processes of phasmid neurons (PHA, PHB, and PHC), interneurons (PVC, DVA, DVB, PVQ, PVT) and motor neurons (VD13, DD6, VA12) are candidate sites for accumulation of DKF-1 protein. |
Expressed in neuronal cell bodies and/or processes. |
|
50-70 cell embryo(author) = 51-cell embryo(curator). early embryo(author) = blastula + gastrulating embryo(curator). fragment altered 7/97, at request of IHope late embryo(author) = 2-fold embryo(curator). life_stage summary : each cell-group has different pattern |
|
Expr21
|
The last expression component to appear is in certain cells of the somatic gonad. The D-cells of the vulval labia and unidentified cells of the spermathecal structures begin expression in L4, whilst gonadal morphogenesis is ongoing. The D-cells do not express beyond the first oocyte fertilisations (no zygotes are usually visible when these cells are stained), the spermathecal staining lasting slightly longer into adulthood The next stage at which expression is evident is during the elongation phase of late embryogenesis when the worm is approximately 2 fold. The nuclei of the M2 motor neurones in the terminal bulb of the pharynx stain strongly. More pharyngeal cells show expression as morphogenesis proceeds until at hatching the two I1 interneurones of the metacorpus, either the e2 or m2 cells of the procorpus, and the m8 muscle cell at the pharyngeal-intestinal boundary can all be seen. This pattern remains through the rest of the life cycle, although the m8 expression is lost during early larval stages These early larval stages also see the appearance of expression in the tail region. The nuclei of the anal sphincter cell and 3/4 neuronal cells of the posterior ganglia comprise this regional component of the pattern This gene gives rise to a complicated multicomponent developmental expression pattern. Earliest expression is seen during the cleavage stage of embryogenesis, in the clonal descendants of the E blastomere, the founder cell giving rise the whole of the gut of the adult animal. Expression begins in Ea and Ep just after gastrulation, and continues into each of the granddaughters of these two cells. At this stage, the expressing cells clearly outline the emerging form of the gut. This component ends at about the 150/200 cell stage |
|
|
Neuronal gene expression pattern from collation by Shawn Lockery of neuron-specific promotors posted 20/04/98 (http://chinook.uoregon.edu). Since cell AS is listed under 'body' in the pattern, it presumably means the AS.1-11 ventral cord neurons rather than the AS amphid neurons.[sdm-curator] |
|
Expr320
|
head: IL2 URA URB SAA SAB SIA SIB SMB SMD RMD AIY, sev more; phar: M1 M2 M5 I1f I6f, others; body: VA VB VC DA DB AS SDQ HSNf; tail: ALN PLN others [J. Duerr (personal communication to Shawn Lockery)], antibody |
|
|
|
|
Expr9584
|
hlh-14 is first expressed at the AB64-cell stage. hlh-14 expression appears to persist longer in posterior parts of the ABalppp/praaa lineage than in anterior parts. In the ASE branch, located at the posterior part of the lineage, hlh-14 expression is maintained throughout the lineage until shortly before the terminal division of the ASE neurons. In addition to its expression in the ASE lineage, it was observed by 4Dlineage analysis that the hlh-14 fosmid reporter is expressed in several other neuronal lineages. In some instances this expression is bilaterally symmetric, such as in the neuroblasts that give rise to the L/R bilaterally symmetric PLM/ALN neuronal pairs, PVQ/HSN/ PHB neuron pairs and the ALM/BDU neuron pairs. Surprisingly, in other instances this expression is L/R asymmetric, such as in neuroblasts of the C lineage. We observe asymmetric expression in two asymmetrically generated neuroblasts, the Caapa neuroblast that gives rise to the DVC neuron and a cell death, and the Caappv cell, which differentiates into the neuron PVR. The bilateral homologs of these hlh-14-expressing neuroblasts are non-neuronal hypodermal cells. Intriguingly, we also observe expression in the bilaterally symmetric neuroblasts that produce a pair of pharyngeal interneurons known as I1L/I1R. Finally, we observe later expression of hlh-14 in currently unidentified lineages, at a time in which all terminally differentiated cells present at hatching are already formed. |
|
