WormMine: AnatomyTerm WBbt:0004949

• Definition: Process runs along excretory canal, no synapses, essential for survival
• Name: CANL
• Primary Identifier: WBbt:0004949
• Synonym: lineage name: ABalapaaapa

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABalapaaapa	ABalapaaapa nucleus		WBbt:0001881

2 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Top 300 transcripts enriched in CANL, CANR according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:CAN
	Single-cell RNA-Seq cell group 69_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:69_0

135 Expression Patterns

• Primary Identifier: Expr4900
• Remark: Picture: Figure 4.
• Pattern: UNC-69::GFP expression was first detectable in embryos. In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular ganglia in the head, and in neurons of the preanal, dorso-rectal and lumbar ganglia in the tail. The fusion protein was also present in the ventral nerve cord (VNC), in the dorsal nerve cord (DNC), in the dorsal and ventral sublateral nerve cords, and in commissural axons. The reporter was expressed in the neurons named CAN, HSN, ALM, PLM, AVM, PVM, BDU, and SDQR, as evidenced by its localization to the cell bodies of these neurons. Expression of unc-69 in these latter cells was confirmed using an unc-69::LacZ::NLS fusion.
• Subcellular Localization: In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in the cell bodies of neurons.

• Primary Identifier: Expr4592
• Pattern: The full-length dyf-5::gfp construct (see Expr4591) showed weak GFP expression in many neurons in the head, including amphid and labial sensory neurons and three pairs of neurons in the tail, including the phasmid sensory neurons. In addition, expression was observed in many cells in the male tail. The dyf-5ex4::gfp fusion construct essentially showed the same dyf-5 expression pattern, albeit stronger and more restricted to the cell bodies. In addition, DYF-5ex4::GFP could be detected in the CAN cells, neurons associated with the excretory canal and in a pair of neurons in the posterior lateral ganglion.
• Subcellular Localization: This DYF-5::GFP fusion could be detected uniformly in axons, cell bodies, and dendrites. In addition, authors observed strong fluorescence at the transition zones, which connect the cilia with the dendrites, and weak fluorescence uniformly in the cilia.

• Primary Identifier: Expr8656
• Remark: Picture: Fig 3.
• Pattern: The fusion protein encoded by the transgene was preferentially detected in several tissues including the nervous system. However, its presence was not uniform. Within the nervous system, ELPC-1::GFP was seen predominantly in a pair of neurons that control egg-laying, the HSNs, and in chemosensory neurons in the head. Within the latter class of neurons, the ELPC-1::GFP level was particularly high in the ASE, ADF and ASK pairs of neurons. Outside of the nervous system, a strong ELPC-1::GFP signal was seen in the pharynx and the vulva. In all animals examined, ELPC-1::GFP expression was also seen in the two CAN cells.
• Subcellular Localization: In the nervous system, expression was seen both within the cell bodies and along the entire lengths of the neuronal processes. Outside of the nervous system, in all cells in which ELPC-1::GFP was seen, fluorescence was restricted to the cytoplasm.

• Primary Identifier: Expr15619

• Primary Identifier: Expr8970
• Remark: Picture: Fig 7.
• Pattern: GFP expression was observed in several neurons including head neurons, motor neurons located in the ventral nerve cord, HSN and CAN neurons, and tail neurons. However, rep-1 does not seem to be expressed in all the neurons. Not only was GFP involved in the neuronal expression, it was also expressed in various muscles such as body-wall, pharyngeal, intestinal and anal sphincter, in addition to the seam cells, hypodermis and the intestine.

• Primary Identifier: Marker49
• Remark: Picture: N.A. Reporter gene fusion type not specified.
• Pattern: Expressed in anterior neurons, including AIY, AIZ, RID, M5, ASI, and labial sensory neurons, VNC motorneurons, midbody neurons HSN, CAN, and PVM, tail neurons DVB, DVC, and PDB, and the nonneuronal excretory cell, uterine muscles. -- according to pers. comm. from Oliver Hobert.

• Primary Identifier: Expr11753
• Remark: genomic

• Primary Identifier: Expr15558

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15608

• Primary Identifier: Expr15611

• Primary Identifier: Expr8851
• Remark: Picture: Fig 3.
• Pattern: Neuronal Expression: labial sensory, CAN, phasmid sheath, a few head neurons. Non-neuronal Expression: seam cells, pharyngeal marginal and muscle cells, arcade cells.

• Primary Identifier: Expr11299
• Pattern: Expressed in body wall muscle, pharyngeal muscle and marginal cells, gut, distal tip cell, uterus, CAN, excretory cell (not visible on most images), coelomocytes, several neuronal and non-neuronal cells in head and tail, strong expression at all stages including embryos

• Primary Identifier: Expr15244

• Primary Identifier: Expr15247

• Primary Identifier: Expr15249

• Primary Identifier: Expr15253

• Primary Identifier: Expr15255

• Primary Identifier: Expr9452
• Remark: Operon: CEOP1368
• Pattern: Animals bearing the transcriptional and translational reporters had similar GFP expression patterns. L1 animals carrying the translation reporter expressed GFP in many neurons, including CANs, DD-type motoneurons and ALMs. Expression in the nervous system began early in comma-stage embryos and peaked in intensity around the 3-fold stage of embryogenesis. Although neuronal expression was much fainter at later larval stages, it persisted in some head and tail neurons through adulthood. Non-neuronal cells that also expressed CRML-1::GFP included the migrating distal tip cells, the pharynx, some vulval epithelial cells, rectal epithelial cells and the excretory canal.
• Subcellular Localization: Sub-cellular localization within the body wall muscle: Muscle cell membrane +/- Muscle arms

• Primary Identifier: Expr9224
• Pattern: mtm-9::gfp reporter was expressed in a broad range of tissues, including the muscle, intestine, hypodermis and neurons (including the CAN neuron in the mid-body region). Mtm-9 was also expressed in the rectal epithelial cells that are the major source of EGL-20 Wnt.

11 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 290-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage when embryo just finished gastrulation and is enclosing.		enclosing embryo Ce	WBls:0000013
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016
	The C. elegans life stage spanning 210-350min after first cleavage at 20 Centigrade. Proliferate from 421 cells to 560 cells. The stage before the fast cleavage of cells finishes.		late cleavage stage embryo Ce	WBls:0000014

2 Parents

Definition	Name	Synonym	Primary Identifier
embryonic cell	ABalapaaap		WBbt:0006493
Neuron class of two neurons that are closely associated with the excretory canal.	CAN		WBbt:0006827