WormMine: AnatomyTerm WBbt:0003812

• Definition: Neuron, process runs along excretory canal and into ring, unique darkly staining synaptic vesicles
• Name: BDUL
• Primary Identifier: WBbt:0003812
• Synonym: lineage name: ABarppaappp

1 Children

Definition	Name	Synonym	Primary Identifier
nucleus of pedigree ABarppaappp	ABarppaappp nucleus		WBbt:0001763

2 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Top 300 transcripts enriched in ALML, ALMR, BDUL, BDUR according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:ALM_BDU
	Single-cell RNA-Seq cell group 80_0 expressed in neuron.	scVI 0.6.0	WBPaper00065841:80_0

78 Expression Patterns

• Primary Identifier: Expr4900
• Remark: Picture: Figure 4.
• Pattern: UNC-69::GFP expression was first detectable in embryos. In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular ganglia in the head, and in neurons of the preanal, dorso-rectal and lumbar ganglia in the tail. The fusion protein was also present in the ventral nerve cord (VNC), in the dorsal nerve cord (DNC), in the dorsal and ventral sublateral nerve cords, and in commissural axons. The reporter was expressed in the neurons named CAN, HSN, ALM, PLM, AVM, PVM, BDU, and SDQR, as evidenced by its localization to the cell bodies of these neurons. Expression of unc-69 in these latter cells was confirmed using an unc-69::LacZ::NLS fusion.
• Subcellular Localization: In immature neurons, UNC-69::GFP expressed in the processes and growth cones of developing neurites. In older larvae and adults, UNC-69::GFP was expressed in the cell bodies of neurons.

• Primary Identifier: Expr15649

• Primary Identifier: Expr13039

• Primary Identifier: Expr15558

• Primary Identifier: Expr15567

• Primary Identifier: Expr15571

• Primary Identifier: Expr15572

• Primary Identifier: Expr15573

• Primary Identifier: Expr15579

• Primary Identifier: Expr823
• Remark: F22A3.3 = glr-8
• Pattern: I1, I2, MC, NSM, M3 (left/right), I3, MI M4, M3 (left/right), I6, M5, BDU, ALM, PLM, URB (left/right)

• Primary Identifier: Expr2937
• Pattern: Both ahr-1:GFP reporters are expressed during embryonic and larval development. Expression is first detected in two cells 260 min after the first cleavage. By midembryogenesis (pre-comma stage), 14 cells express the pJ360 ahr-1:GFP fusion gene. At the 2-fold stage of embryogenesis, two cells express ahr-1:GFP in the tail, and the remaining fluorescing cells are in the forming head. During the first larval stage. ahr-1:GFP is expressed in 28 neurons, several blast cells, and two phasmid socket cells. The neurons that express ahr-1:GFP include ALNR/ALNL, AQR/PQR, AVM/PVM, BDUR/BDUL, PLMR/PLML, PLNR/PLNL, PHCL/PHCR, PVWL/PVWR, RMEL/RMER, SDQR/SDQL, and URXR/URXL. The T.pa, T.ppa, and T.ppp blast cells in the tail express ahr-1:GFP, as do all of their descendents, including the PHso1 and PHso2 phasmid socket cells. ahr-1:GFP is also expressed in the MI and I3 neurons in the pharynx and the G2 and W blast cells. Four additional cells in the head express ahr-1:GFP, tentatively identified as the ASK and RIP neurons.
• Subcellular Localization: The pJ360 construct includes the entire ahr-1 genomic sequence, and transgenic animals express this fusion protein in a subset of neuronal nuclei. The pHT102 transgene lacks most of the ahr-1 coding sequence and labels axons as well as nuclei.

• Primary Identifier: Expr15586

• Primary Identifier: Expr15651

• Primary Identifier: Expr15652

• Primary Identifier: Expr15589

• Primary Identifier: Expr15591

• Primary Identifier: Expr15598

• Primary Identifier: Expr15604

• Primary Identifier: Expr15608

• Primary Identifier: Expr15369

• Primary Identifier: Expr15611

• Primary Identifier: Expr11628
• Pattern: KIN-19::RF was most highly expressed in the pharynx and in a pair of neuronal processes identified by their position as BDU neurons. With age, the formation of fluorescent-KIN-19 puncta was observed throughout the anterior pharyngeal bulb, indicative of KIN-19 aggregation. KIN-19::tagRFP puncta were found throughout the cytoplasm in pharyngeal muscle and marginal cells. KIN-19::tagRFP was highly expressed in dorsal, ventral, and lateral neuronal processes.

• Primary Identifier: Expr733
• Remark: This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope).
• Pattern: Staining is seen in a set of 47 nuclei in fixed newly hatched first larval stage (L1). All stained cells are neurons. Hermaphrodite express in cells. RIH, RIR, PVR, IL2L/R, URYVL/R, RIPL/R, AIZL/R, FLPL/R, ADAL/R, RMGL/R, BPUL/R, PLML/R, ALML/R, ALNL/R, HSNL/R, URBL/R, NSML/R, URADL/R, IL2DL/R, I2L/R, IL2VL/R, URAVL/R, URXL/R, AIML/R, URYDL/R, PQR, PVM, SDQL/R, PVDL/R, PHCL/R, PLNL/R. Male cells express as in hermaphrodite except for HSNL/R which die, and show expression in CEMDL/R, CEMVL/R which die in hermaphrodites. Expression pattern is first determined in the Q lineage. Once expression has been initiated in a cell, it is maintained by that cell and all of its descendants in all cases except for SDQ.

• Primary Identifier: Expr14012
• Pattern: ASK, ASI, ASH, ASJ, one pair posterior to AWB (some space in btw), PHA, PHB, one pair just posterior to PHB (sometimes dimmer), HSN, AVM, BDU, Amso, ray expression in males

• Primary Identifier: Expr9974
• Pattern: GFP fluorescence appeared exclusively neuronal during development from about the 1.5 fold stage of embryogenesis through to the adult. No obvious RHGF-2s::GFP fluorescence was visible in the earlier stages of embryogenesis. Many, but not all, of the 302 C. elegans hermaphrodite neurons expressed GFP in the transgenic animals examined. Several cell bodies in head and tail ganglia and many neuronal processes in the nerve ring and ventral cord were visible, with the GFP fluorescence for most cells distributed diffusely throughout the cell, although there were instances of more punctate fluorescence within some cell bodies and processes, particularly in the ventral cord. Neurons that were positively identified in late larval/adult animals based on their position and morphology were the ALM, AVM, PLM and PVM mechanosensory neurons and the BDUs. Expression from the ciliated sensory neurons, most ventral cord motor neurons and neurons with cell bodies in the mid-body of the animal such as the CANs, PDEs and SDQs, for example, was notably absent from transgenic animals observed at late larval and adult stages.

• Primary Identifier: Expr14693
• Pattern: A two-color splicing reporter for sad-1 in C. elegans revealed that many neurons express both the skipped and included isoforms. For example, motor neurons in the ventral nerve cord express both isoforms of sad-1. On the other hand, the ALM touch-sensing neuron expresses only the included isoform, while the BDU neuron, which is the sister cell to the ALM neuron, expresses only the skipped isoform.

• Primary Identifier: Expr3310
• Pattern: Expression was detected first in neuronal precursor cells at the comma stage. Slightly later strong expression was found in the hypodermal cells. In newly hatched L1 larvae this construct continued to be expressed in neuronal cells in the head and tail, in cells of the ventral nerve cord and of the pharynx. During the early L1 stage hypodermal expression disappeared. The GFP-positive neurons in animals carrying pT14G10.2::GFP-II were identified as the RMDD, RMD, RMDV, SMDD, and SMDV, which are ring motoneurons. Also the more posteriorly located BDU cells and the ALN, PVR, and PVT cells in the tail expressed GFP. Analysis of synchronized worms showed that GFP is present around the time of molting, but is barely detectable during part of the intermolts or in adults. During the outgrowth of the gonadal primordium expression was seen in the distal tip cell and oviduct sheath cells.

• Primary Identifier: Expr1146
• Remark: Reporter gene fusion type not specified.
• Reporter Gene: [cam-1::gfp]. A functional cam-1gfp transgene that rescues the defects of cam-1 mutants.
• Pattern: CAM-1GFP expression appears at the 200-cell stage in most cells of the embryo. Migrating ALM, BDU, CAN, HSN and ccM cells, which migrate embryonically and require cam-1 function for their migration, are likely to express CAM-1GFP, as most cells of the embryo express the transgene while these cells migrate. During the first larval stage, V cells often express CAM-1GFP at the time that they divide, and the Q-neuroblast descendents, which also require cam-1 function for their migration, express CAM-1GFP. During larval development, CAM-1GFP is highly expressed in the nervous system, as well as in intestinal, hypodermal and body-wall muscle cells and in parts of the pharynx.
• Subcellular Localization: In non-neuronal cells, much of the protein appears to associate with the plasma membrane. In neurons, CAM-1GFP is detected predominantly in axons and dendrites.

• Primary Identifier: Expr1316
• Pattern: AVM, PDE, PLM, BDU, PVC, RIP, pharynx, body wall muscle, vulva muscle.

• Primary Identifier: Expr7866
• Remark: Picture: Figure 5A to 5E.
• Pattern: This UNC-36::GFP reporter was expressed in most neurons and virtually all muscle tissue (consistently in body wall and vulval muscle, and sometimes in the pharyngeal muscle). Expression of the UNC-36 reporter was observed in mechanosensory neurons, as well as a number of additional unidentified neurons in the head and tail. GFP expression was observed in ALM, AVM, BDU, and SDQR. Also identified in the tail neurons PVQ, PVC, DVC, and DVA. PLM, ALN, and PHB were probable, but not certain. In the head GFP was expressed in ASE, AVA, SIBDL, RMDVL, ASK, and a number of unidentified neurons. Expression was also observed in PVM and SDQL.

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003
	The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo.		fully-elongated embryo Ce	WBls:0000021
	The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over.		elongating embryo Ce	WBls:0000015
	The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.		3-fold embryo Ce	WBls:0000020
	The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.		1.5-fold embryo Ce	WBls:0000018
	The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.		2-fold embryo Ce	WBls:0000019

3 Parents

Definition	Name	Synonym	Primary Identifier
cell that exists in and is part of an embryo.	embryonic cell		WBbt:0007028
embryonic cell	ABarppaapp		WBbt:0006393
Neuron class of two interneurons with cell bodies situated laterally in the anterior body.	BDU		WBbt:0006826