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Expr4381
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Expressed in AVA, AVB, DVA, I5, RID, RIM, PVQ, SAA, SIA, SIB, SMB(?), SMD, ventral cord motor neurons, some unidentified neurons in the head. Also expressed head muscles (weak), body wall muscles (weak). |
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Expr15649
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The differences in the expression patterns of Pmbr-1::gfp and mbr-1::gfp might be due to the following reasons: first, additional regulatory elements might exist in introns and 3$(B!l(B-UTR regions, which are not included in the former construct; and second, as the product of Pmbr-1::gfp diffuses throughout the cytoplasm, it might be difficult to detect low levels of GFP expression. |
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Expr3681
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In strains that carry Pmbr-1::gfp, GFP expression was observed in restricted sets of interneurons in the head ganglia: AIM, RIC, RIH (or RIR), RIF (or RIG), and a pair of interneurons tentatively identified as AIN. Expression was also observed in three interneurons of the tail ganglia: PVP and an interneuron tentatively identified as DVA or DVC. In contrast, mbr-1::gfp was expressed in several more neurons compared to Pmbr-1::gfp, including sensory neurons. Expression of mbr-1::gfp was also detected in some intestinal cells at the late embryonic stages as well as in vulval cells and some somatic gonadal cells at the L4 stage. mbr-1::gfp expression becomes detectable first around the early comma stage, which corresponds to the time just after the birth of most neurons. |
The MBR-1::GFP fusion protein was localized to the nuclei, consistent with the idea that MBR-1 functions as a transcription factor. |
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Expr13039
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Expr15558
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Expr9325
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Synaptogyrin is expressed in all 26 GABAergic neurons including also RMER and most though not all other neurons. Synaptogyrin is absent in amphids and phasmids and can be detected in non-neuronal glial-like sheath cells in adult worms. The cephalic neurons CEPDR/L and CEPVR/L and amphid-associated sheath cells CEPshDR/L, CEPshVR/L were tentatively positive. Several other neurons that could be tentatively identified in the anterior part are MI, M4, I4, AVL, AIY, RIS, I5, M3R/L, and in the posterior part DVA, AS11, ALNR/L, DVC, DVB, PQR, DA9 (characteristic axonal process denoted by arrowhead), VD13, DD6, VD12. Of these, AVL, RIS, VD13, DD6 and VD12 are GABAergic based on the colocalization with the unc-47p::GFP reporter. In addition, IL neurons were tentatively identified in the anterior (IL*). Synaptogyrin reporter constructs are also expressed in developing neurons. The expression of sng-1p::YFP is closely associated with the development of the nervous system being absent in the gastrula stage with first fluorescence in neuronal precursor cells and newly-formed neurons in the anterior part during the 1.5-fold stage. In addition, it is also detected transiently in cells in the posterior body at the 1.25-fold and 1.5-fold stage. |
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Expr15567
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15652
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Expr15589
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Expr13158
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Expr15591
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Expr13164
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For lgc-38, all expressing cells shown are observed with the 3.5 kb reporter fusion, except for OLL, which only expresses the 3.9 kb fusion; URA expresses both. |
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Expr15598
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr963
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Transgenic animals bearing pFX1G1 had high levels of GFP fluorescence or immunoreactivity in embryonic and postembryonic neurons. fax-1::gfp expression was first detected in embryos prior to elongation (approximately 350 minutes of development). By approximately 400 minutes, there is strong fax-1::gfp expression in as many as 20 neurons in the embryonic head and 1-2 neurons in the embryonic tail. fax-1::gfp is expressed in 20 neurons postembryonically, through the adult stage. The position of these neurons indicates that most or all of them are among the 22 neurons that express fax-1::gfp embryonically. These cells include both AVKR and AVKL. fax-1::gfp was not observed in either of the HSN or PVQ neurons, or in the PVPR neuron at any stage of development. fax-1::gfp expression was observed in several other neurons and two non-neuronal cell types in transgenic animals carrying pFX1G1. These include the pairs of CEPD and URX sensory neurons, three pharyngeal neurons (M1, MI and probably M5), two pairs of ring interneurons (including the RIC pair), five neurons in the retrovesicular ganglion (including SABD and the pair of SABV neurons), a single neuron in the preanal ganglion (either PVPL or PVT) and a single neuron in the dorsorectal ganglion of the tail (probably DVA). There is incompletely penetrant fax-1::gfp expression in a few additional neurons that were not identified, and in the non neuronal dorsal rectal cell and distal tip cells of the somatic gonad. |
GFP immunoreactivity was present in the cytoplasm, axons and nuclei of cells. Axons of neurons that express fax-1::gfp embryonically were observed in the process of outgrowth. |
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Expr15611
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Expr9837
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Nuclear-localized GFP was seen from the comma-stage embryo through to the mature adult in a variety of cell types. NB. Some variation was found between transgenic lines generated for the same reporter gene fusion clone. GFP was observed in many cells in embryos and from L1 to L3. Neurons in head, tail and ventral nerve cord expressed GFP, DVA particularly strongly in some larvae and adults. Muscle cell nuclei throughout the body contained GFP, with higher levels in the M lineage from the L1. In some L1s, L2s and L3s, GFP expression was observed in the P lineage although this was variable presumably due to mosaicism of the transgenic array. GFP was also seen in the developing vulva and reproductive muscles. Intestinal expression of GFP was present in L3s, L4s and adults, but inconsistently. |
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life_stage summary : postembryonic |
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Expr8
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Post-embryonic expression. L1-L4, single ventral cell in middle of the second bulb of the pharynx. L1, single cell in dorsal midline near nerve ring, L2-young adult, 1 dorsal and 1 ventral cell near nerve ring. L2/L3 cell in dorsorectal ganglion. |
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Expr12599
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Broad expression of the frpr-4::gfp translational reporter was observed during both larval development and adulthood; expression was in body wall muscles, pharyngeal muscles, and multiple neurons, including the RIA neurons, the DVA neuron, and the PVM neuron. Membrane localization of the green fluorescence was observed, as would be expected for a GPCR. |
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Expr10831
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Particularly intense snf-5 EGFP signal was observed in intestinal cells INT1-9 of the alimentary canal. EGFP expression exhibited a more intensive and aggregated pattern in the epithelial cells of the mature gut, but more even and diffuse expression in the alimentary canal of younger worms. EGFP signal was detected in the pharynx in some, but not all, analyzed preparations. EGFP expression was also detected in the posterior cells of the alimentary canal, two cells of the rectal gland, and several cells that approximately colocalize with the positions of the DVA, DVB or DVC neurons. Further identification of these cells will require additional analysis. Expression of EGFP reporter was also identified in three pairs of amphid sensory neurons that are localized near the dorsa-anterior surface of the posterior pharyngeal bulb (pbp). Considering the localization to the lateral ganglia of head, the relative somatic positions identified via 3D reconstruction of confocal sections, the axonal projections, and the projections and shapes of dendrites visualized in a maximum projection of confocal stack these cells represent the ASI, ADF and ASK neurons. The ASI and ADF neurons exhibited intense labeling in the somata and along the axonal and dendritic projections. In contrast, ASK labeling was weak in the somatic regions and neuronal branches. EGFP expression was also detected in a population of small neurons at the dorsal surface of the anterior pharyngeal bulb. |
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Expr14034
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ADL (dim), AVL, RIS, head mesodermal cell, pharynx (dim), pharyngeal-intestinal valve (dim), anal depressor muscle, stomatointestinal muscle, PVT, DVA, distal tip cell, CAN |
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Picture: Fig 3. |
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Expr8678
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Expression in the alimentary canal: Strong and consistent expression in pharyngeal epithelium, pm1, pm2, pm3, pm6, pm7, pm8, mc1, mc2, mc3. Weak or rare expression in pm4, pm5. Expression in the nervous system: DDn, DVA, DVB, DVC, PVP. Expression in the reproductive system: In adult stage, expressed in proximal gonad sheath, spermatheca. In developing larva stage, expressed in uterus, spermatheca. inx-10 is localized to pharyngeal precursors from early stages of embryogenesis, and by three-fold stage, all pharyngeal muscles except pm4 are seen to express it at high levels. |
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