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Expr4431
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Expressed in the seam cells at adult stage. Expressed in the rectal epithelial cells only in the fourth larval stage (L4). Expressed in VulB and VulD. Expressed in anterior arcade cells. Expressed in a pharyngeal neuron in the metacorpus extending a neurite anteriorly and an axon posteriorly. |
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Expr4405
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At the beginning of vulva morphogenesis, a strong expression from the plx-1::gfp transcriptional reporter is found in all migrating vulva cells. |
As vulva morphogenesis progresses, expression from the plx-1p::PLX-1::GFP translational reporter increases at the plasma membrane of migrating vulva cell. However, although some signal is found on the entire cell membrane, PLX-1::GFP appears to be predominantly localized on the vulva center facing membrane (future lumen surface) of primordial vulva cells destined to enter the vulva proper. At the end of morphogenesis, PLX-1::GFP is predominantly expressed in the most ventral vulva rings [vulA, vulB1, vulB2, vulC and vulD (which are P5.p and P7.p derived)] and the signal is localized along the lumen formed by these cells. |
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Since utse aff-1 expression and AC-utse fusion occur almost simultaneously, it is possible that aff-1 expression detected in the utse is actually a contribution from the AC cytoplasm after the fusion event. To test this, authors examined utse aff-1 expression in lin-29(n482) mutant worms where AC-utse fusion does not occur. aff-1 expression in the utse in these mutants indicated that aff-1 is specifically expressed in the utse cells and is not a consequence of AC to utse cytoplasmic GFP diffusion after fusion. |
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Expr4654
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Specific and continuous expression was detected in the AC from the invasion of the vulval primordium at mid-L3 until its fusion with the utse cells. As the vulva completes its invagination in the L4, the utse syncytium starts to express aff-1, resulting in coexpression of aff-1 in both cells prior to their fusion. Thus, AFF-1 is dynamically expressed in a specific group of cells that undergo cell fusion during normal development. aff-1 expression is first detected in the embryonic hyp5 cell and later during larval development in various cell types, including pharyngeal muscles (Pm3 and Pm5), uterine rings (Ut2 and Ut4), head and tail neurons, sheath cells of chemosensory neurons, and male tail neurons). aff-1 is also expressed in vulval vulD and the seam cells shortly before these cells fuse. In general, the myoepithelial cells of the pharynx and the epithelial cells in the uterus, vulva, and hypodermis that express aff-1 undergo fusion. Thus, AFF-1 is dynamically expressed in a specific group of cells that undergo cell fusion during normal development. |
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Expr4655
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AFF-1::GFP was weakly expressed on the plasma membrane and in intracellular organelles in hyp5, seam cells, vulD vulval precursors, and AC before, during, and after cell-cell fusion. |
Expressed on the plasma membrane and in intracellular organelles. |
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Reporter gene fusion type not specified. |
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Expr4657
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Expressed exclusively in the vulD cells. |
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Expr4645
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In the presence of the 6-kb promoter region, the vulval expression is identical to that of the 8-kb nhr-67::[Delta]pes-10 constructs (see Expr4642). Besides the previously reported expression in head neurons, expression was also observed in the anchor cell (AC) (during mid to late L3 stage) in hermaphrodites and the linker cell in males. |
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Expr4642
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Expression was observed in the vulva, the hyp7 epidermal syncytium, late stage embryos, and the male tail. This nhr-67 construct exhibits a dynamic expression pattern in the vulval cells. During the late L4 stage, nhr-67 is first observed in vulA cells (and occasionally in vulB1), and this expression is maintained throughout adulthood. Expression in vulC is only seen upon entry into L4 lethargus and persists in adults. Strong vulB1 and vulB2 expression (and occasional vulD expression) is observed only in young adults. |
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Expr4455
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Expressed in the hypodermis, mainly in the embryo. Expressed in the seam cells only in larval stages. Expressed in VulB, VulC, VulD and VulE. |
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For dre-1::gfp, a 4 kb promoter was amplified with primers 5' -GGTACCCGAGGGGACATCGAGATAG-3' and 5' -GGTACCTTCCTGGCCAACCAGAGAC-3' and was cloned into Fire vector L3781 (BA 279). The dre-1 ORF and the dre-1 3' UTR region were amplified with primers 5' -GCTAGCATGTCGTCCTCTTCGTCAC-3' and 5' -ACTAGTTACTTACTCCACTCCACACAG-3' and were cloned into BA279 (BA280). Lines containing this construct included dhEx346 and dhIs442. To obtain the full-length promoter construct, authors substituted the promoter from BA279 with a 12.3 kb promoter by using primers 5' -GCGGCCGCGTTGCACACAAAACATTATTATTTTCTTTCTCTT-3' and 5' -TACGTATCTCGTCCCTGAGATCTCTCATTT-3' (BA508). Resulting lines containing this array included dhEx443 and dhEx452. --precise ends. |
Expr4547
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First detected by midembryogenesis, expression was most prominent in epidermal and intestinal cells. By the 1.5-fold stage of embryogenesis, expression was additionally detected in neurons and other cells. During larval and adult stages, DRE-1::GFP was most visible in epidermal seam cells and hypodermis. Expression was high in larvae and low in adults. In addition, DRE-1 was strongly expressed in the P epidermal blast cells and descendents that give rise to the vulva. Weak expression was seen in the somatic gonad, including the gonadoblasts, the anchor cell, dtcs, and occasionally adult spermatheca and uterus. Notably, with another construct (dhEx346, 4 kb promoter, 4 kb coding region), dtc expression was stronger and commenced by mid-L3. In the musculature, DRE-1 was seen in the pharynx, anal depressor, sex muscles, and body wall muscles. Finally, DRE-1 was detected in neurons of the head, tail, ventral cord, and periphery. |
Generally, DRE-1::GFP was localized to both the nucleus and cytoplasm, and it was broadly expressed, including in phenotypically affected tissues. |
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Expr4502
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A transcriptional reporter for sel-2 in which the 5' upstream region drives nuclearly localized YFP is expressed in the VPCs and their descendants, as well as in many other cell types, including the epithelial cells of the intestine and the rectum, the seam cells, many cells in the head and the tail, and the cells of the ventral nerve cord. |
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Most of the tissues expressing rcn-1 overlap with those observed in calcineurin GFP and by immunostaining, including lateral hypodermal cells, vulva muscle tissue, nerve cords, and diverse neuronal expression. Reporter gene fusion type not specified. |
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Expr2548
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GFP analysis of the transformed animals revealed that rcn-1 is expressed from late embryonic stages to adulthood in diverse tissues, including lateral hypodermal cells, marginal cells of the pharynx, vulva epithelial cells, ventral and dorsal nerve cords and commissures, and various neurons in the anterior and posterior portions of the worm. Male C. elegans displayed rcn-1 expression in male tail structures including the diagonal muscles, sensory rays, and spicules. GFP expression analysis of a shorter 1.6 kb 50 upstream fragment of rcn-1 revealed vulva muscle expression in addition to the aforementioned expression patterns. |
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All of the reporter constructs produced the same cell-specific expression pattern as transgenes. |
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Expr1438
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The reporter transgenes express ubiquitously in the early embryo starting at about the 100 cell stage during gastrulation. In late embryogenesis and posthatching, expression is more limited. Strongest expression is observed in migrating cells and growing neurons as these cells undergo movements on the epidermis. At hatching, the reporters express in many neurons throughout the animal, in several cells of the pharynx including some pharyngeal neurons, in the elongated processes of the excretory cells, in the amphid and phasmid sheath and socket cells, in the tail hypodermis, and at later stages in intestine, muscles, vulva, and somatic gonad including the gonad sheath and hermaphrodite distal tip cells. The neurons expressing unc-73 include the PLM, ALM, PDE, HSN, CAN, PHC, and PVN neurons and the ventral cord motorneurons. Expression in the HSNs is absent in early larval stages, but begins late in the second larval stage (L2), precisely when axon outgrowth is initiated from the HSN cell bodies. The Q neuroblasts, Pn neuroectoblasts, sex myoblasts (SMs), and canal associated neurons (CANs) express unc-73 reporters. The left and right Q cells begin to express the GFP reporter as they initiate their migrations along the longitudinal axis of the epidermis during the early first larval (L1) stage, and expression in these cells continues beyond the completion of their first division. The unc-73 reporters express in the Pn cells just before this second phase of movemen. The distal tip cells also express the unc-73/reporters during their migration. |
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Other strains-- UL530, UL531 late embryo(author) = 1.5-fold + 2-fold + 3-fold + fully-elongated embryo(curator). Legacy data: Author "Bauer PK" "Mounsey A" "Hope IA". Date 1999-11. |
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Expr136
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The first component of expression consists of much diffuse and nuclear-localised staining which persists from post-comma-stage embryos through to the L2 stage. In young larvae expression is seen in many cells, including hypodermis, intestinal, neuronal and pharyngeal. In older larvae and adults staining is less extensive and is confined to a subset of hypodermal cells of the head and body, the vulC and vulD cells of the vulva, pharyngeal staining, and areas just distal to the uterus (probably the spermathecae). |
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The specificity of the antibody staining is indicated by the following controls: first, the staining is competed away by preincubation of the antibody with GPB-1 protein and, second, gpb-1 mutant embryos do not exhibit any staining. |
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Expr2747
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In situ immunohistochemistry with this antiserum shows that GPB-1 is present in early embryos. Diffuse staining can be seen at the 1-cell stage. From the 2-cell stage onward. Up to the beginning of morphogenesis, staining continues at approximately equal levels in most or all cells. Once tissue differentiation occurs, staining is brightest in neuronal cells. In larval and adult stages, GPB-1 expression is seen in most or all neurons, including the nerve ring, the dorsal and ventral nerve cords, and the preanal ganglion. In addition, the somatic gonad, vulva, and hypodermal seam cells have high expression. Other tissues, such as the intestine, pharynx, and body wall muscles, appear to have a low level of expression. GPB-1 is also expressed in the germline. |
GPB-1 is detected most strongly at the cell membrane, with staining concentrated at the contact between cells. Interestingly, staining also colocalizes with the asters (arrays of microtubules emanating from the centrosomes) just before and during early cell divisions. |
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Expression of gfp in sEx13706 animals is directed by a 2.8 kb hda-1 regulatory region that includes the open reading frames and potential cis-regulatory elements(enhancers) of two other hda-1 upstream genes (ril-1 and C53A5.2). The other hda-1::gfp transgenic strain (bhEx72), generated in this study, contains a much smaller 5'upstream region of hda-1 (approximately 1.0 kb, pGLC44) and excludes the two genes mentioned above. The analysis of GFP fluorescence in sEx13706 and bhEx72 animals revealed a similar pattern, although the fluorescence in sEx13706 was much brighter. |
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Expr11136
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hda-1 is broadly expressed throughout development. The earliest expression was detected in gastrulating embryos. The larvae exhibited GFP expression in several neuronal and epidermal cells, primarily in the anterior ganglion and ventral hypodermal regions. Expression persisted in many cells in later larval and adult stages (data not shown). In the vulva, hda-1::gfp expression was first detected in the progeny of P(5-7).p in mid-L3 animals. At this stage, GFP fluorescence was absent in other VPC lineages (P3.p, P4.p and P8.p) (data not shown). By the L4 stage, almost all vulval cell types were observed fluorescing, with presumptive vulA, vulB1, vulB2, and vulD cells being the brightest. GFP fluorescence in vulval cells was mostly absent beyond the late-L4 stage, suggesting that hda-1 may not be needed in vulval cells at later stages of development. The broad expression of hda-1 is in consistent with the involvement of the gene in multiple developmental processes. This multifaceted role for hda-1in C. elegans appears to be conserved in C. briggsae, because Cbr-hda-1::gfp is expressed in a similar manner. hda-1::gfp expression was also observed in the AC in L3 animals that persisted until the early L4 stage (data not shown). No expression was observed in pi cells or their progeny at any developmental stage. |
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Expression of gfp in sEx13706 animals is directed by a 2.8 kb hda-1 regulatory region that includes the open reading frames and potential cis-regulatory elements(enhancers) of two other hda-1 upstream genes (ril-1 and C53A5.2). The other hda-1::gfp transgenic strain (bhEx72), generated in this study, contains a much smaller 5'upstream region of hda-1 (approximately 1.0 kb, pGLC44) and excludes the two genes mentioned above. The analysis of GFP fluorescence in sEx13706 and bhEx72 animals revealed a similar pattern, although the fluorescence in sEx13706 was much brighter. |
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Expr11137
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hda-1 is broadly expressed throughout development. The earliest expression was detected in gastrulating embryos. The larvae exhibited GFP expression in several neuronal and epidermal cells, primarily in the anterior ganglion and ventral hypodermal regions. Expression persisted in many cells in later larval and adult stages (data not shown). In the vulva, hda-1::gfp expression was first detected in the progeny of P(5-7).p in mid-L3 animals. At this stage, GFP fluorescence was absent in other VPC lineages (P3.p, P4.p and P8.p) (data not shown). By the L4 stage, almost all vulval cell types were observed fluorescing, with presumptive vulA, vulB1, vulB2, and vulD cells being the brightest. GFP fluorescence in vulval cells was mostly absent beyond the late-L4 stage, suggesting that hda-1 may not be needed in vulval cells at later stages of development. The broad expression of hda-1 is in consistent with the involvement of the gene in multiple developmental processes. This multifaceted role for hda-1in C. elegans appears to be conserved in C. briggsae, because Cbr-hda-1::gfp is expressed in a similar manner. hda-1::gfp expression was also observed in the AC in L3 animals that persisted until the early L4 stage (data not shown). No expression was observed in pi cells or their progeny at any developmental stage. |
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Expr3419
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The functional mrp-1::GFP gene was expressed not only in the pharynx, pharynx-intestinal valve cells, anterior intestinal cells, intestinal-rectum valve cells and epithelial cells of the vulva, but also in some neurons, as well as other intestinal cells and hypodermal seam cells. Expression was detected already in the pharynx, intestine and neurons in L1 larvae. |
Localized in cell membrane. |
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Expr1899
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Expression of the construct is first detected during embryogenesis, at the beginning of morphogenesis. evl-20::gfp reporter is strongly expressed in the migrating hypodermal cells throughout the enclosure process. Shortly after the beginning of elongation, evl-20::gfp is turned on in many developing neurons, where it persists through adulthood. (This pattern reflects zygotic evl-20 expression, and it is possible that the maternally produced endogenous gene product is also present in the early embryo. However, such maternal expression cannot be recapitulated by the reporter construct due to the germline silencing effect.) In larva, the construct is expressed in a subset of developing tissues. The reporter construct is highly expressed in all 22 cells of the vulva in mid L4 stage. No apparent difference in levels of expression between cells adopting primary and secondary vulval fates can be seen. evl-20::gfp expression can first be detected in P5.p to P7.p during the two-cell stage, after the first round of VPCs divisions. GFP expression was observed in the somatic gonad during L2 to L4 stages. The expression was the strongest in the uterus, although it was also detectable in the spermatheca, sheath cells, and the distal tip cells (DTC). Expression of the construct in the vulva and the gonad was undetectable in adult worms. GFP expression is also seen in many cells during male tail development, with the highest levels found in the proctodeum. As mentioned previously, evl-20 is also expressed in most neurons during larval stages and in the adult worm. |
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Other strain-- UL403 late embryo(author) = elongating embryo + fully-elongated embryo(curator). |
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Expr122
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Expression begins in precomma stage embryos. It is quite strong, with extensive diffuse cytoplasmic staining as well as nuclear localised staining. Expression is strongest in young larvae, with staining observed in the ventral nerve cord, the circumpharyngeal nerve ring, the head ganglion, the tail ganglion, the retrovesicular ganglion, and in the developing vulva. In older larvae and in adults the strong pharyngeal expression seen in young larvae is less intense and some neuronal processes in the head become apparent (e.g. the motorneuron M1). There is also staining in the pharyngo-intestinal valve and in the seam cells, though expression appears to exclude the nuclei and is generally intermittent along the seam. The defecation muscle group stain as does its axon, DVB. The dorsal cord also stains but is very faint. Two commissures stain (these are also faint), one is located anterior to the vulva, and the other is posterior to the vulva. |
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Other strain-- UL747, UL756, UL757 |
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Expr140
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A less frequently observed components is of nuclei in the tail which is most likely hypodermis. A less frequently seen component is in vulC and vulD of L4 larvae and adult worms. Expression seen in unidentified groups of cells in elongating embryos. The most common and obvious component of this expression pattern is the strong staining in the terminal bulb of the pharynx (m6 and m7). Also fainter expression in the m2 nuclei of the procorpus. The most common and obvious component of this expression pattern is the strong staining in the terminal bulb of the pharynx (m6 and m7). Other less frequently observed components are-- vulC and vulD in L4 and adult worms, nuclei in the head and tail which are most likely hypodermis, m2 nuclei in the procorpus, and unidentified patches of staining in elongating embryos. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr700
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beta-gal staining detected in all developmental stages including embryos and is most intense in early larval stages. Although NLS was present in the construct between the promoter sequences and lacZ gene, beta-gal staining was found in the cytoplasm and at the membrane. mrp-1 expression is observed in cells of the pharynx, the pharynx-intestinal valve cells and the anterior intestinal cells, the epithelial cells of the vulva and the rectum-intestinal valve cells. Similar tissue distribution is observed with mrp-1::gfp. In L3, staining seen in pharynx, pharynx-intestinal valve and first intestinal cells, intestinal-rectum valve cells. In young adults, staining is detected in epithelial cells of the vulva. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). pPCUbi6 containing animals show various degrees of gonad abnormality and produce few progeny. |
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Expr729
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pPCubi3 Expression is observed in the intestine, nerve ring body muscle, hypodermis, pharynx, pharyngeal-intestinal valve, vulva and weak expression in the ventral nerve cord. No expression is seen in embryos. ppCubi6 Expression is observed at a higher level than pPCUbi3 animals. With additional expression seen in all somatic cells of gonad, including distal tip cells, sheath cells and cells of spermatheca and uterus. Also expression is seen throughout the embryos. |
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Expr9214
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NCK-1B isoform was expressed exclusively in the vulva cells of the developing vulva, pharynx, intestine, distal tip cells (DTCs), gonad arms and spermatheca. In embryos, NCK-1B-GFP is expressed in the intestine, nervous system, epidermal cells and pharynx. In adult animals, NCK-1B-GFP is expressed in the nerve ring, amphid neurons, ventral nerve cord (VNC) and dorsal nerve cord (DNC), SDQ neurons, mechanosensory neurons (PVM and AVM), as well as in the CAN and HSN neurons. NCK-1B is also expressed in non-neuronal cells. NCK-1B is expressed in vulB1, vulB2, vulA, vulF and vulD cells, as well as in the leading edge (arrows) of the migrating vulva cells. In addition, NCK-1B is expressed in the spermathecal-uterine junction (sujn). |
The NCK-1B isoform was localized to the cytoplasm and nucleus. The nuclear localization appeared to be strong. |
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lima stage (author) = bean embryo (wjc). |
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Expr1074
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NID-1 was first detected in embryos at the beginning of morphogenesis (lima stage) localized to body wall muscle cells. As embryos elongate strong NID-1 staining is seen around body wall muscle cells and diffuse stain begins to accumulate on the surfaces of the pharyngeal and intestinal primordia. Once the embryo has elongated to the 2-fold stage, NID-1 has localized to the basal face of the body wall muscles and shows strong accumulation on the surfaces of the pharyngeal, intestinal, and gonad primordia. In three- and fourfold stage embryos, NID-1 accumulates to higher levels and remains localized under the four body wall muscle quadrants and on the surfaces of the pharynx, intestine, and gonad. In L1 larvae the intensity of staining of body wall muscle, pharynx, and intestine appears reduced, and strong staining associated with the nerve ring becomes apparent. This pattern continues through the L2 and L3 larval stages, with the addition of stronger staining of the distal tip cells as they lead the growth of the gonad. In late L3 to L4 stage larvae particularly strong NID-1 accumulation is seen associated with the distal tip cells and the developing somatic structures of the gonad, the spermatheca, uterus, and vulva. Under the body wall muscles of larval and adult stage animals NID-1 is organized as punctate lines. These lines follow the rows of dense bodies within the muscle cells. NID-1 also accumulates strongly at the outer edges of the muscle quadrants and more weakly at the boundaries between muscle cells within each quadrant. Less organized NID-1 staining is seen in the regions between the body wall muscle quadrants, presumably associated with the epidermal basement membranes in these regions. NID-1 accumulates along the four sublateral nerves that run beneath the center of each muscle quadrant. The sublateral nerves extend along dorso- and ventrolateral tracts from the nerve ring in the anterior of the animal to near the middle of the animal where they turn further lateral to positions coincident with the lateral edges of the body wall muscle quadrants. NID-1 accumulation on these nerves is seen in larval and adult animals and is similar in intensity to the staining at the edges of the body wall muscle quadrants. Staining along the edges of the body wall muscle quadrants appears to be associated with the muscle edges rather than the nerves in these regions because the staining closely follows the edge of the muscles and it does not display the left/right asymmetry expected for the ventral and dorsal nerve cords. Less organized NID-1 staining is also present in the regions between body wall muscle quadrants. |
membranes |
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Expr1455
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Almost every transgenic animal shows strong DAF-3/GFP expression in many, but not all, head neurons, the ventral nerve cord (both cell bodies and processes), the intestinal cells, especially the membrane adjacent to the intestinal lumen, and tail hypodermal cells and neurons. Weak expression in the pharynx, hypodermal V blast cells, P blast cells and hyp7 hypodermal cells is observed in about half of the transgenic animals. Expression in the tail hypodermal cells hyp 9, hyp 10, and hyp 11 is clearly seen in nearly every animal. This apparent difference between tail hypodermal expression and main body expression may be a consequence of the anatomy of the animal. The main body hypodermis is underlain by bright GFP in the intestine and ventral nerve cord, so weak expression in the hypodermis is hard to see against this background. Expression is rarely detected in dorsal body wall muscle. DAF-3/GFP is expressed in the distal tip cells and in their precursors, Z1.a and Z4.p, throughout development. DAF-3/GFP is also expressed strongly in unidentified vulval cells in adults. In wild-type embryos of 200 to 400 cells, DAF-3/GFP is expressed uniformly thoughout the embryo. |
In wild type, DAF-3/GFP is primarily, although not exclusively, cytoplasmic. DAF-3/GFP subcellular distribution was examined in head neurons in the vicinity of ASI (the cell that produces the DAF-7 signal), as well as in intestinal cells. DAF-3/GFP was predominantly cytoplasmic in all animals. However, in all animals, dim GFP fluorescence was seen in the nucleus of some of the cells with bright cytoplasmic fluorescence, and in 25% of the animals, equivalent DAF-3/GFP levels in the nucleus and cytoplasm was observed in one or more cells. |
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Expr2291
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SQV-4 Abs stained the cytoplasm of many cells, including (but not limited to) oocytes and vulval cells, as well as uterine, seam, pharyngeal, and spermathecal cells. During the early L4 stage, 10 of the 22 vulval nuclei were in cells with dramatically increased expression of SQV-4. These 10 nuclei are the six inner nuclei of the P5.p and P7.p descendants and the four outer nuclei of the P6.p descendants. During the mid-late L4 stage and thereafter, cells containing the inner four nuclei of the P6.p descendants also increased SQV-4 expression, bringing the total of vulval nuclei that highly expressed SQV-4 to 14. Thus, the 22 vulval nuclei define three classes based on the levels and timing of SQV-4 expression. |
cytoplasm |
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50-70 cell embryo(author) = 51-cell embryo(curator). early embryo(author) = blastula + gastrulating embryo(curator). fragment altered 7/97, at request of IHope late embryo(author) = 2-fold embryo(curator). life_stage summary : each cell-group has different pattern |
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Expr21
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The last expression component to appear is in certain cells of the somatic gonad. The D-cells of the vulval labia and unidentified cells of the spermathecal structures begin expression in L4, whilst gonadal morphogenesis is ongoing. The D-cells do not express beyond the first oocyte fertilisations (no zygotes are usually visible when these cells are stained), the spermathecal staining lasting slightly longer into adulthood The next stage at which expression is evident is during the elongation phase of late embryogenesis when the worm is approximately 2 fold. The nuclei of the M2 motor neurones in the terminal bulb of the pharynx stain strongly. More pharyngeal cells show expression as morphogenesis proceeds until at hatching the two I1 interneurones of the metacorpus, either the e2 or m2 cells of the procorpus, and the m8 muscle cell at the pharyngeal-intestinal boundary can all be seen. This pattern remains through the rest of the life cycle, although the m8 expression is lost during early larval stages These early larval stages also see the appearance of expression in the tail region. The nuclei of the anal sphincter cell and 3/4 neuronal cells of the posterior ganglia comprise this regional component of the pattern This gene gives rise to a complicated multicomponent developmental expression pattern. Earliest expression is seen during the cleavage stage of embryogenesis, in the clonal descendants of the E blastomere, the founder cell giving rise the whole of the gut of the adult animal. Expression begins in Ea and Ep just after gastrulation, and continues into each of the granddaughters of these two cells. At this stage, the expressing cells clearly outline the emerging form of the gut. This component ends at about the 150/200 cell stage |
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Expr1164
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Transgenic animals that carry this construct show GFP expression in a variety of tissue types. GFP expression is observed in the intestine, and the posterior cells express GFP more intensely than the remaining intestinal cells. Other cells include the rectal epithelial cells, the pharynx, the somatic gonad, and vulval hypodermal cells. In addition, the IP3 receptor is expressed in hypodermal cells of the tail, rectum, and head. Pharyngeal expression is restricted to the muscles of the metacorpus, isthmus, and the anterior portion of the terminal bulb (m4, m5, and m6). This construct was only expressed in neurons LUA and PDA. GFP is expressed in the gonad sheath cells, spermatheca, spermathecal valve, and uterine sheath cells. GFP is expressed in the vulval hypodermal cells. |
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Reporter gene fusion type not specified. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr689
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let-60 ras::lacZ. The Vulval lineage: First detected in L3 larvae (before vulval induction). Faint staining observed in P3.p-P8.p. Staining becomes stronger as VPCs begin dividing and fusion protein is expressed through adulthood. Faint staining observed in hyp7. Strong staining in vulA, vulB, vulC, vulD, vulE and vulF. Myoblast lineage: L1 (shortly after division of M) - Staining detected in M.d and M.v. Late L1, faint staining in progeny of M.v (body muscle) including SM (progeny of M.d (body muscle) ceases staining). L3: 8 progeny of SM (vulval muscle) stain before and after differentiation in muscle cells. Gonadal lineage: At hatching Z1 and Z4 gonadal cells stain. Progeny Z1 and Z4 that form distal tip cells (dtcs) and dtcs stain throughout larval development-adulthood. L4: Anchor cells (ac) of somatic gonad stains transiently at apex of invaginating vulva and continues until late L4 when ac nucleus fuses with uterine tissue. L4-adult expression (but not lacZ) Intense gfp near germline nuclei along border of distal arm of the gonad and in some places extended into the rachis. Muscle: L1-adult: All body wall muscle cells stain. Pharyngeal muscle pmp3-8 begin staining in L1 and continue until adulthood. Hypodermis: Begin staining in L2-3 larvae but consistent staining does not occur until the L4 stage and continues until adulthood. Hypodermal cells staining include V and H lineage-derived seam cells and V and H derived lateral hypodermal cells. Ventral hypodermal cells derived from P lineage also stain weakly but consistently in the adult. Intestine: Intestinal cells of late L1 larvae stain briefly during their terminal division. No staining after L2. Nervous system and excretory cells: extensive staining but not entirely at hatching throughout development. Beginning L1 - adulthood: Many ventral cord neurons stain positively identified include FLPL,R AVKL,R and either AIMR or AIYR based on co-staining with an anti-FMRF amide and an anti-galactosidase antibody. Nucleus of excretory cell stains in L1 to adulthood. |
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Expr3011
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Expressed in AIM, ASI, AUA, BAG, BDU, DVB, PQR, PVR, URX, vulD. No male specific expression. |
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