WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

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Anatomy Term :

Definition  one member of Amphid neurons, single ciliated endings, probably chemo-sensory; project into ring via commissure from ventral ganglion, make diverse synaptic connections in ring neuropil Name  ASJL
Primary Identifier  WBbt:0003886 Synonym  lineage name: ABalpppppppa

1 Children

Definition Name Synonym Primary Identifier
nucleus of pedigree ABalpppppppa ABalpppppppa nucleus   WBbt:0001685

1 Expression Clusters

Regulated By Treatment Description Algorithm Primary Identifier
  Top 300 transcripts enriched in ASJL, ASJR according to single cell RNAseq. Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm. WBPaper00061340:ASJ

177 Expression Patterns

Remark Reporter Gene Primary Identifier Pattern Subcellular Localization
    Expr4403 Strong and consistent expression was observed in a limited number of neurons in the head and tail and coelomocytes. Weaker and/or inconsistent expression of TTX-7::EGFP was detected in nerve cord motor neurons, intestine, and somatic gonad. The head neurons expressing ttx-7::EGFP include AFD and RIA neurons. Also expressed in ASH, ASE, ASJ, AWC, ADF, ADL, ASI, ASK, AWB, VNC motor neurons, etc. TTX-7::EGFP was diffusely expressed in the cytoplasm and was not localized to any specific subcellular compartment.
    Expr4270 INS-1::Venus, which was confirmed to be functional, was expressed in several amphid chemosensory neurons, ASE, ASI, and ASJ; AIA interneurons; and several other neurons. Of these expression patterns, the fluorescence signal in AIA was the strongest and most consistent between individual animals. In AIA neurons, punctate expression patterns in the cell bodies and processes were observed.
    Expr4225 Consistent with the previously described microinjections of the non-GFP constructs, only the full-length ssu-1::GFP fusion rescued the suppression of the locomotion defect of unc-1(null) by ssu-1(fc73). However, both constructs produced strong fluorescence in only two head neurons. Using the amphid stain DiI, these two head neurons were identified as the ASJ amphid neurons.  
A translational DYF-2::GFP fusion was found to be localized only in cilia of CSN, thus precluding a confirmation of the cellular expression pattern obtained with the transcriptional dyf-2::gfp fusion or any further cell identification.   Expr4204 dyf-2::gfp expression was observed in only a subset of the ciliated sensory neuron (CSN) class in the worm. GFP signal was observed in seven out of twelve neurons of the amphids, including ASH, ASI, ASJ, ASK, ADL (ciliated neurons that fill with the fluorescent dye DiI) plus two hitherto unidentified amphid neurons. In addition, GFP expression was observed in the phasmid CSN and in neurons identified as AQR and PQR (asymmetric CSN in the head and tail, respectively). No or only very occasional GFP signal was detected in other CSN anterior to the nerve ring in the head of the worm.  
    Expr4541 Expressed in ASKL/R, ASIL/R, ASJL/R.  
    Expr4511 RGS-3::GFP expression is seen in seven pairs of head sensory neurons (ASH, ADL, AWB, AWC, ASI, ASJ and ASK) as well as PHA and PHB in the tail.  
Reporter fusion construct for the translational fusion not specified.   Expr11200 Upstream regulatory sequences of cng-2 drove gfp expression exclusively in six pairs of head sensory neurons in the adult. AWC, ASE, ASG, ASI, ASJ, ASK (Fig. 2B). When expressed under an ASK-specific promoter the CNG-2 fusion protein was restricted to the ASK cell bodies.
    Expr15649    
    Expr3175 Expressed in ADF, ADL, AFD, ASE, ASG, ASH, ASI, ASJ, ASK, AWA, AWB, AWC, BAG, PHA, PHB, URX, intestine.  
Picture: Fig 4E to 4H. npr-5 = Y58G8A.4 in this paper.   Expr8976 The npr-5p::RFP transgene was expressed in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB) in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Outside the nervous system, npr-5p::RFP was expressed in head, neck, and body muscles throughout larval and adult development.  
genomic   Expr11753    
Isoform 1b.1   Expr11755    
Isoform 1b.2   Expr11756    
    Expr3169 Expressed in ADF, ADL, ASH, ASI, ASJ, ASK, PHA, PHB.  
    Expr3185 Expressed in ADF, ADL, ASE, ASG, ASH, ASI, ASJ, ASK, PHA, PHB, URX, labial neurons.  
    Expr15558    
The cellular expression pattern for nlp-3 fragments was indistinguishable from the cellular expression pattern for nlp-3 subcloned constructs. Transgenic lines were generated by coinjection of the nlp::gfp construct (5070 ng/ul) and lin-15 rescue construct (pJM24,100 ng/ul) into lin-15(n765ts) animals. At least two independent transgenic lines were analyzed for each nlp gene; 5-10 animals were scored per line. 1,1'-Dioctadecyl-3,3,3',3 -tetramethylindodicarbocyanine perchlorate (Molecular Probes) was used to stain amphid and phasmid neurons to facilitate identification.   Expr1688 Expressed in ADF, ASE, ASH, AWB, ASJ, BAG, HSN, I1, I2, I3, I4, MI, M3, NSMR, 3 head neurons, VNC, oocytes, I6, M2, pm1VL, intestine.  
    Expr15571    
    Expr15572    
    Expr15573    
    Expr15579    
    Expr15586    
    Expr15651    
    Expr15652    
    Expr15589    
    Expr15591    
    Expr15598    
    Expr15604    
    Expr15606    
    Expr15608    

8 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. embryo Ce WBls:0000003
  The C. elegans life stage spanning 620-800min(hatch) after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. A stage after elongation is over. The last stage of embryogenesis. Also called pre-hatched embryo, late embryo or morphogenetic embryo. fully-elongated embryo Ce WBls:0000021
  The C. elegans life stage spanning 350-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The stage that embryo starts elongation until elongation is over. elongating embryo Ce WBls:0000015
  The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage. 3-fold embryo Ce WBls:0000020
  The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo. 1.5-fold embryo Ce WBls:0000018
  The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo. comma embryo Ce WBls:0000017
  The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo. 2-fold embryo Ce WBls:0000019
  The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage. bean embryo Ce WBls:0000016

6 Parents

Definition Name Synonym Primary Identifier
neuron with its cell body situated in the head, excluding the pharynx. head neuron   WBbt:0006751
cell that exists in and is part of an embryo. embryonic cell   WBbt:0007028
embryonic cell ABalppppppp   WBbt:0006599
Neuron class of two ciliated neurons that are part of the amphid sensilla. The endings are in the amphid channel, which is open to the outside. ASJ   WBbt:0005667
neuron type, cell body located in left lateral ganglion. lateral ganglion left neuron   WBbt:0005102
  amphid left sensillum   WBbt:0005395