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Expr4287
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psa-3 was expressed in the T cell, head neurons, posterior gut cells, hypodermal cells (hyp7, hyp9, and hyp10), and P blast cells. Of the seam cells, only the T cell expressed psa-3. During mitosis of the T cell, the PSA-3 protein was uniformly distributed in the T cell. Soon after the T cell division (before V6 cell division, which occurs about 30 min after T cell division), the psa-3 expression in the two daughter cells was almost the same. In the later stage, after V6 cell division, the psa-3 expression in T.a had decreased and that in T.p increased. After the next round of divisions, the psa-3 expression had greatly increased in the posterior (T.pa and T.pp), but not the anterior, granddaughters. |
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Expr2937
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Both ahr-1:GFP reporters are expressed during embryonic and larval development. Expression is first detected in two cells 260 min after the first cleavage. By midembryogenesis (pre-comma stage), 14 cells express the pJ360 ahr-1:GFP fusion gene. At the 2-fold stage of embryogenesis, two cells express ahr-1:GFP in the tail, and the remaining fluorescing cells are in the forming head. During the first larval stage. ahr-1:GFP is expressed in 28 neurons, several blast cells, and two phasmid socket cells. The neurons that express ahr-1:GFP include ALNR/ALNL, AQR/PQR, AVM/PVM, BDUR/BDUL, PLMR/PLML, PLNR/PLNL, PHCL/PHCR, PVWL/PVWR, RMEL/RMER, SDQR/SDQL, and URXR/URXL. The T.pa, T.ppa, and T.ppp blast cells in the tail express ahr-1:GFP, as do all of their descendents, including the PHso1 and PHso2 phasmid socket cells. ahr-1:GFP is also expressed in the MI and I3 neurons in the pharynx and the G2 and W blast cells. Four additional cells in the head express ahr-1:GFP, tentatively identified as the ASK and RIP neurons. |
The pJ360 construct includes the entire ahr-1 genomic sequence, and transgenic animals express this fusion protein in a subset of neuronal nuclei. The pHT102 transgene lacks most of the ahr-1 coding sequence and labels axons as well as nuclei. |
