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Expr4536
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Expressed in IL2 (strong), ASEL/R (faint), additional faint sensory neurons (three pairs). |
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Picture: Fig 4E to 4H. npr-5 = Y58G8A.4 in this paper. |
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Expr8976
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The npr-5p::RFP transgene was expressed in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB) in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Outside the nervous system, npr-5p::RFP was expressed in head, neck, and body muscles throughout larval and adult development. |
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Expr12393
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Pcil-7::GFP was expressed in CEM, RnB, HOB, and IL2 neurons. |
The translational and functional CIL-7::GFP reporter localized to cell bodies (excluding nuclei), dendrites, axons, and the cilia and ciliary bases of the extracellular vesicle (EV)-releasing sensory neurons -CEM, RnB, HOB, and IL2. |
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Expr9983
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Pnphp-2::GFP was expressed in the ciliated sensory nervous system throughout development. In the adult, expression was evident in both hermaphrodite and male ciliated sensory neurons, including amphid, phasmid, and IL2 neurons. nphp-2 was also expressed in male specific ciliated sensory nervous system, including the CEM, RnB, and HOB neurons. Expression in the internal oxygen sensor neuron PQR was visible in the hermaphrodite, but could not be distinguished from the tail sensilla in the male. |
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genomic |
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Expr11753
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Expr15284
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We observed prominent dma-1p::dma-1::gfp expression in the IL2 dendrites during dauer. Expression of dma- 1p::dma-1::gfp was not detectable in adult IL2s. dma-1p::dma-1::gfp translational reporter is prominently expressed in the FLPs and PVDs during dauer. We observed prominent dma-1p::dma-1::gfp expression in the IL2 dendrites during dauer. Expression of dma- 1p::dma-1::gfp was not detectable in adult IL2s. |
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Expr15558
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15589
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Expr15591
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Expr13164
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For lgc-38, all expressing cells shown are observed with the 3.5 kb reporter fusion, except for OLL, which only expresses the 3.9 kb fusion; URA expresses both. |
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Expr15598
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr15611
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Expr15436
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unc-17(3k) |
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Expr11217
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kpc-1 is expressed in numerous neuronal and epithelial cells during dauer. Processes adjacent to the body wall in dauers resemble those of IL2Q 4 dendrites. To determine whether kpc-1 is expressed in the IL2s, we coinjected the Pkpc-1::GFP reporter with a Pklp-6::tdTomato reporter that is expressed exclusively in the IL2s. During nondauer stages, kpc-1 is not expressed in the IL2 neurons (data not shown). In dauers, Pkpc-1::GFP and Pklp-6::tdTomato are coexpressed, indicating that kpc-1 is upregulated in the IL2s during dauer. Nondauer expression was consistently observed in the ventral nerve cord and pharynx with strong expression in the g2 pharyngeal gland cells and vpi pharyngeal intestinal valve cells. |
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To compare the transcription pattern of the daf-1 promoter with the whole gene, the gfp cDNA was fused to the terminus of the daf-1 gene in a plasmid that included 2.6 kb of upstream sequence. Despite the fact that this transgene rescued the daf-1 Daf-c mutant phenotype, GFP fluorescence was not detected in rescued animals. Hence, these observations were limited to the daf-1 promoter fusion, which may not represent the expression pattern of the whole daf-1 gene if enhancer elements are present in introns or in 3' sequences. |
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Expr946
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GFP expression was observed in the head and the developing ventral nerve cord beginning in mid-stage embryos and continuing into adulthood. In the head, GFP was detected in more than twenty neurons in the anterior, lateral, ventral and retrovesicular ganglia. Fluorescent processes terminating at the tip of the head suggest that daf-1 is expressed in sensory neurons and in support cells in the amphids and inner labial sensilla. In the midbody, GFP was expressed in the ALM mechanosensory neurons and the PVT neuron, as well as one additional neuron pair. In the lumbar ganglia of the tail, five cells expressing GFP included phasmid neurons and PLN and PLM mechanosensory neurons. The daf-1 promoter also conferred gfp expression in nonneuronal cells, including a membranous sheath surrounding the distal end of the intestine and in the distal tip cell (DTC) of the gonad. In some lines, GFP was sometimes detected in the muscles of L4 and adult animals. In L1 larvae, daf-1 promoters is active in neurons in the head, as well as in the ventral cord and tail. The promoter continues to express GFP in dauer larvae from starved plates. |
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This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr733
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Staining is seen in a set of 47 nuclei in fixed newly hatched first larval stage (L1). All stained cells are neurons. Hermaphrodite express in cells. RIH, RIR, PVR, IL2L/R, URYVL/R, RIPL/R, AIZL/R, FLPL/R, ADAL/R, RMGL/R, BPUL/R, PLML/R, ALML/R, ALNL/R, HSNL/R, URBL/R, NSML/R, URADL/R, IL2DL/R, I2L/R, IL2VL/R, URAVL/R, URXL/R, AIML/R, URYDL/R, PQR, PVM, SDQL/R, PVDL/R, PHCL/R, PLNL/R. Male cells express as in hermaphrodite except for HSNL/R which die, and show expression in CEMDL/R, CEMVL/R which die in hermaphrodites. Expression pattern is first determined in the Q lineage. Once expression has been initiated in a cell, it is maintained by that cell and all of its descendants in all cases except for SDQ. |
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Expr15502
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rab-28 is expressed in the IL2 neurons present in both males and hermaphrodites, as well as all 21 male-specific extracellular vesicle releasing neurons (EVNs). |
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Expr11704
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Expression of the transcriptional fusion Ptop-1::gfp was not detected in early embryonic cells due to maternal germline silencing of the multi-copy transgenic gene. The GFP expression, however, was observed in most cells at the late embryonic stage and decreased with larval development. In the larval stages, GFP expression was predominantly present in the neuronal system, including sensory neurons (ILso, URX, RIC, IL1/IL2, AIY/AIM and RIG/RIF), motor neurons (VC4, VC5, HSN, PVD and PVM) of hermaphrodites and tail neurons (SPD and SPV/SPC) of males. The expression of the transcriptional fusion Ptop-1::gfp was strong in DTCs during the L3-L4 stage when gonad migration proceeds. |
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Expr9653
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CCPP-1::GFP was neuronally expressed in developing embryos (not shown) through adulthood in amphid and IL2 ciliated sensory neurons of the core nervous system, which males and hermaphrodites have in common. In the male-specific nervous system, CCPP-1::GFP was coexpressed with pkd-2 in CEM head neurons and the RnB and HOB neurons in the tail. CCPP-1::GFP was also expressed in some unidentified neurons and the gubernacular erector and retractor muscles in the male tail. CCPP-1::GFP was local- ized diffusely throughout neurons, including cilia, but excluded from the nucleus. CCPP-1::GFP was neuronally expressed in developing embryos (not shown) through adulthood in amphid and IL2 ciliated sensory neurons of the core nervous system, which males and hermaphrodites have in common. In the male-specific nervous system, CCPP-1::GFP was coexpressed with pkd-2 in CEM head neurons and the RnB and HOB neurons in the tail. CCPP-1::GFP was also expressed in some unidentified neurons and the gubernacular erector and retractor muscles in the male tail. CCPP-1::GFP was localized diffusely throughout neurons, including cilia, but excluded from the nucleus. |
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Other Strains: OH14267 / OH14268 |
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Expr14068
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IL2, ASK, ASI (dim), ASG, one pair above ASJ, pharynx, sometimes PVT, PHA and one other non-phasmid pair, ray expression in males |
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Reporter gene fusion type not specified. |
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Expr3222
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Epidermis: ncr-1Ap(l)::gfp was strongly expressed in the excretory cell and rectal epithelial cells from the early L1 larval stage and through all life stages. Seam cell expression was first observed in the late L1 stage, while expression in the lateral hypodermis increased during the L3 stage and peaked during the L4 stage. Seam cell and hypodermal expression gradually decreased in the adult stage and was hardly visible among older adults. ncr-1Ap(s)::gfp was not expressed in the hypodermis under normal growth conditions, though lateral hypodermal but not seam cell expression was dramatically upregulated in starved animals of all developmental stages. No increase in hypodermal expression was seen in starved ncr-1Ap(l)::gfp animals. Intestine: Both ncr-1Ap(s)::gfp and ncr-1Ap(l)::gfp were strongly expressed throughout the intestine, with posterior intestinal expression consistently stronger than anterior expression. Musculature: ncr-1Ap(l)::gfp was strongly expressed in pharyngeal muscles, but not in body wall muscles. Nervous system: ncr-1Ap(s)::gfp and ncr-1Ap(l)::gfp were expressed in the same set of head and tail neurons and a pair of neuron-like cells identified as the XXX cells. According to their location and cellular morphology, the head neurons were identified as the pharyngeal neuron I6, the inner labial sensory neurons IL2s and the amphid neurons ASE and ASG. The expression level in the amphid neurons was weaker than in the other head neurons. The tail neurons were identified as PHC, in which expression was first detected during the L2 stage, consistent with the time of birth of the neurons at the end of L1. In contrast to the widespread expression of ncr-1Ap::gfp, ncr-1Bp::gfp is expressed exclusively in 10-12 pairs of head and tail neurons. The tail neurons were identified as PHA, PHB and DVC. One pair of head neurons was identified as AWC. The other head neurons were very tentatively identified as RIC, RIM, FLP, ADA, ADE, RID and maybe AIY. We also occasionally observed expression in a pair of head cells anterior to the nerve ring. The position and morphology of these cells are similar to the XXX cells. With the exception of PHC neurons, expression in the tissues above was first observed during late embryogenesis and did not change during development. Somatic gonad: Both ncr-1Ap(s)::gfp and ncr-1Ap(l)::gfp were strongly expressed in the spermatheca and weakly in the gonadal sheath cells. The expression in the somatic gonad could be observed only in adults. Three reporter constructs, ncr-1Ap(s)::gfp, ncr-1Ap(l)::gfp and ncr-1Bp::gfp were made for ncr-1 because of its complex gene structure. The results indicate that ncr-1 expression is widespread and largely coincides with the reported distribution pattern of cholesterol in C. elegans, which includes the following tissues: intestine, pharynx, excretory gland cell, nerve ring, spermatheca and germ cells, including both oocytes and sperm. |
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