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late embryo(author) = fully-elongated embryo(curator). life_stage summary : L4/adult moult, vulval muscles life_stage summary : from late embryo , pharynx |
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Expr35
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The second component is in the vm2 vulval muscles. The expression here is cytoplasmically localised, and covers only the period around the L4 to adult molt This gene has two distinct modes of expression. The earlier component is in a subset of cells of the pharynx, and is nuclear localised. Expression here is from late embryogenesis onwards, the subset consisting of the m4 muscles in the mid metacorpus,the m2 muscles and e1 and e2 epithelial cells of the procorpus, and the m8 muscle of the posterior terminal bulb. |
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50-70 cell embryo(author) = 51-cell embryo(curator). early embryo(author) = blastula + gastrulating embryo(curator). fragment altered 7/97, at request of IHope late embryo(author) = 2-fold embryo(curator). life_stage summary : each cell-group has different pattern |
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Expr21
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The last expression component to appear is in certain cells of the somatic gonad. The D-cells of the vulval labia and unidentified cells of the spermathecal structures begin expression in L4, whilst gonadal morphogenesis is ongoing. The D-cells do not express beyond the first oocyte fertilisations (no zygotes are usually visible when these cells are stained), the spermathecal staining lasting slightly longer into adulthood The next stage at which expression is evident is during the elongation phase of late embryogenesis when the worm is approximately 2 fold. The nuclei of the M2 motor neurones in the terminal bulb of the pharynx stain strongly. More pharyngeal cells show expression as morphogenesis proceeds until at hatching the two I1 interneurones of the metacorpus, either the e2 or m2 cells of the procorpus, and the m8 muscle cell at the pharyngeal-intestinal boundary can all be seen. This pattern remains through the rest of the life cycle, although the m8 expression is lost during early larval stages These early larval stages also see the appearance of expression in the tail region. The nuclei of the anal sphincter cell and 3/4 neuronal cells of the posterior ganglia comprise this regional component of the pattern This gene gives rise to a complicated multicomponent developmental expression pattern. Earliest expression is seen during the cleavage stage of embryogenesis, in the clonal descendants of the E blastomere, the founder cell giving rise the whole of the gut of the adult animal. Expression begins in Ea and Ep just after gastrulation, and continues into each of the granddaughters of these two cells. At this stage, the expressing cells clearly outline the emerging form of the gut. This component ends at about the 150/200 cell stage |
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Expr11043
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F48A11.1 was expressed specifically in the period immediately preceding each moult. Expression appeared limited to the pharynx and specifically to the glandular cells g1 and g2 (5 nuclei) in the terminal bulb, the three m4 myo-epithelial cells (6 nuclei) in the metacorpus and the three m3 myo-epithelial cells (6 nuclei) in the pro- corpus. No GFP was detected elsewhere in transgenic nematodes despite careful examination of the reproductive tissues and intestine. |
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Picture: Fig. 3D. Reporter gene fusion type not specified. |
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Expr8940
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Expressed by the male linker cell from the early L4 stage until LC death in the L4-to-adult molt (n=40/41) |
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[lin-42ap::gfp-pest] transcriptional fusion. The start codon of lin-42a and 3,043 bps of upstream sequence were fused with gfp-pest. --precise ends. |
Expr9861
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GFP expressed from the lin-42a promoter was robustly expressed in the pharyngeal myoepithelium midway through the L1 stage and continuously thereafter. The lin-42a reporter was also expressed in hyp7 and the lateral seam cells of late L1-stage larvae undergoing the first molt. Expression of the lin-42a reporter in the hypodermis was detected later in the L1 stage than expression of the lin-42b reporter. Fluorescence from the lin-42a reporter also persisted through ecdysis, whereas fluorescence from the lin-42b reporter dissipated during lethargus. To fully define the cyclical expression pattern of the lin-42ap::gfp-pest fusion gene, we measured the relative intensity of the associated fluorescence in the hypodermis throughout larval development. The intensity of fluorescence repeatedly increased 2- to 10-fold during the molts and dropped to background levels early in the next life stage. |
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Transgenic Marker: rol-6(su1006). |
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Expr525
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First appears in vulval cells L4-Adult molt, then in seam cells young adult, and non-vulval ventral hypodermal cells of older adults. |
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Expr12489
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abu-14.abu-14::sfGFP fluorescence localizes to the pharyngeal corpus and buccal cavity cuticle. Green fluorescence is seen in both the L4 and adult pharyngeal grinders, in the pharyngeal cuticle, and in the buccal cap. |
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Expr11055
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An nhr-165::gfp translational reporter shows nuclear expression in the lateral hypodermis (hyp7, but not hyp8-11) near the time of larval molts. This expression is highest and seen in the largest number of cells in the tail prior to the L3-L4 molt. |
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Expr12514
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IFA-2::GFP protein localizes to the epidermal fibrous organelles (hemidesmosomes), the uterine seam and touch neurons. Uterine expression includes both utse and uv cells. The seam expression appears at the L4-adult molt and probably correlates with expression of IFA-2 in both the utse and the seam cells as they terminally differentiate. |
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Expr2432
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Detected at a similar level in dauer-L4 and L4-adult molt RNAs, but not in embryos or L2-dauer molt RNAs. |
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Expr2431
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Detected at a similar level in dauer-L4 and L4-adult molt RNAs, but not in embryos or L2-dauer molt RNAs. |
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Expr15165
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nlp-2 mRNA expression peaked in preparation of the L3 and L4 molts, when lin-42 levels are low, suggesting that nlp-2 expression is regulated, at least partially, at the transcriptional level. |
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