WormMine: LifeStage WBls:0000011

• Definition: The C. elegans life stage spanning 120-135 min after first cleavage at 20 Centigrade. Contains 51 cells.
• Primary Identifier: WBls:0000011
• Public Name: 51-cell embryo Ce

52 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
embryonic cell	Cpa		WBbt:0006281
Embryonic founder cell of germ line	Psub4	lineage name: P0.pppp	WBbt:0004421
embryonic cell	Caa		WBbt:0006358
embryonic cell	Cpp		WBbt:0006416
embryonic cell	Cap		WBbt:0006229
embryonic cell	ABplaaa		WBbt:0005905
embryonic cell	ABalpap		WBbt:0005949
embryonic cell	ABprpap		WBbt:0006013
embryonic cell	ABalppp		WBbt:0006048
embryonic cell	ABplpap		WBbt:0006152
embryonic cell	ABpraaa		WBbt:0006228
embryonic cell	ABaraap		WBbt:0006272
embryonic cell	ABalpaa		WBbt:0006338
embryonic cell	ABplaap		WBbt:0006410
embryonic cell	Da		WBbt:0006512
embryonic cell	ABpraap		WBbt:0006548
embryonic cell	ABprpaa		WBbt:0006580
embryonic cell	ABplpaa		WBbt:0006643
embryonic cell	ABaraaa		WBbt:0006691
embryonic cell	Dp		WBbt:0006718
embryonic cell	ABalppa		WBbt:0006741
embryonic cell	Eal		WBbt:0006441
embryonic cell	Ear		WBbt:0006370
embryonic cell	Epr		WBbt:0006547
embryonic cell	Epl		WBbt:0006000
embryonic cell	MSapa		WBbt:0005898
embryonic cell	ABplapa		WBbt:0005879
embryonic cell	ABprapp		WBbt:0005880
embryonic cell	ABplapp		WBbt:0005936
embryonic cell	ABalapp		WBbt:0005946

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The C. elegans life stage spanning 100-290min after first cleavage at 20 Centigrade. Proliferate from 28 cells to 421 cells. Referring to the whole period of gastrulation.		gastrulating embryo Ce	WBls:0000010
	A C. elegans life stage that occurs during the gastrulating embryo life stage		C. elegans life stage occurring during gastrulation	WBls:0000814

0 Expression Clusters

3 Expression Patterns

• Primary Identifier: Expr2021
• Remark: Other strain-- UL968
• Pattern: This highly mosaic pattern has a number of components most of which are seen in only a small fraction of worms. Expression is seen from early embryo to adult stages. Strong diffuse expression is seen in the pharyngeal musculature, with other anterior expression in the intestine, and around the isthmus probably corresponding to the nerve ring. Expression is also seen in and around the vulva, in the vulval muscle cells (vm1 and vm2), and in vulC. Posterior expression around the rectum, probably in the sphincter muscles and anal depressor muscle. Expression is also seen in early embryos (approximately the 50 cell stage) in a number of cells possibly of the AB lineage.

• Primary Identifier: Expr21
• Remark: 50-70 cell embryo(author) = 51-cell embryo(curator). early embryo(author) = blastula + gastrulating embryo(curator). fragment altered 7/97, at request of IHope late embryo(author) = 2-fold embryo(curator). life_stage summary : each cell-group has different pattern
• Pattern: The last expression component to appear is in certain cells of the somatic gonad. The D-cells of the vulval labia and unidentified cells of the spermathecal structures begin expression in L4, whilst gonadal morphogenesis is ongoing. The D-cells do not express beyond the first oocyte fertilisations (no zygotes are usually visible when these cells are stained), the spermathecal staining lasting slightly longer into adulthood The next stage at which expression is evident is during the elongation phase of late embryogenesis when the worm is approximately 2 fold. The nuclei of the M2 motor neurones in the terminal bulb of the pharynx stain strongly. More pharyngeal cells show expression as morphogenesis proceeds until at hatching the two I1 interneurones of the metacorpus, either the e2 or m2 cells of the procorpus, and the m8 muscle cell at the pharyngeal-intestinal boundary can all be seen. This pattern remains through the rest of the life cycle, although the m8 expression is lost during early larval stages These early larval stages also see the appearance of expression in the tail region. The nuclei of the anal sphincter cell and 3/4 neuronal cells of the posterior ganglia comprise this regional component of the pattern This gene gives rise to a complicated multicomponent developmental expression pattern. Earliest expression is seen during the cleavage stage of embryogenesis, in the clonal descendants of the E blastomere, the founder cell giving rise the whole of the gut of the adult animal. Expression begins in Ea and Ep just after gastrulation, and continues into each of the granddaughters of these two cells. At this stage, the expressing cells clearly outline the emerging form of the gut. This component ends at about the 150/200 cell stage

• Primary Identifier: Expr569
• Remark: 50-70 cell embryo(author) = 51-cell embryo(curator). No maternal RNA.
• Pattern: Staining in body muscle precursors at 60-cell through bean embryo.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
Levin M, Hashimshony T, Wagner F, Yanai I. Developmental milestones punctuate gene expression in the Caenorhabditis embryo. Dev Cell. 2012 May 15;22(5):1101-8. Epub 2012 May 3. PubMed PMID: 22560298. Supplementary table 1.	Sixth cleavage of the AB lineage. Contains 68 cells.		68-cell embryo Ce	WBls:0000087

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
Levin M, Hashimshony T, Wagner F, Yanai I. Developmental milestones punctuate gene expression in the Caenorhabditis embryo. Dev Cell. 2012 May 15;22(5):1101-8. Epub 2012 May 3. PubMed PMID: 22560298. Supplementary table 1.	Fifth cleavage of the AB lineage. Contains 44 cells.		44-cell embryo Ce	WBls:0000086

0 Sub Stages