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Reporter gene fusion type not specified. Two GFP reporter constructs were generated, containing distinct putative promoters for nhr-41a and nhr-41b, the latter promoter corresponding to genomic DNA in the large fourth intron. The analysis suggests different expression patterns for the two nhr-41 mRNA isoforms, although it is a formal possibility that enhancers in the fourth intron could be acting on both presumed promoters. Thus, the nhr-41 reporter gene analysis may be revealing distinct functions for the two isoforms. |
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Expr2870
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The nhr-41b reporter gene is expressed consistently in the gut, chemosensory neurons, and head and tail hypodermal cells. |
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Expr12443
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A GFP fusion gene with 7.7 kb of nsy-4 upstream sequence was expressed beginning at the comma stage of embryogenesis and continuing until the adult. At the 2-fold stage of embryogenesis, expression was prominent in the excretory cell and in anterior epidermal cells. At the first larval stage, the nsy-4 reporter transgene was expressed in the excretory cell, in epidermal cells in the head (some or all of hyp 1-6) and tail (some or all of hyp 8-11), and in the P neuro-epidermoblasts, both before and after their ventral migration. Weak nsy-4::GFP expression was detectable in a few neurons. In three appropriate mosaic animals identified at the L1/L2 stage, nsy-4::GFP was observed in the AWC cell body. Expression was not detectable in AWC in older animals. |
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Reporter gene fusion not specified. |
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Expr11444
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Not expressed in NSM nor HSN. Expressed in several dim cells in the head (ADF?) tail and head hypodermal cells. |
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Expr9765
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GFP expression was extremely weak. Although GFP was seen in embryos and L1 in all strains, in UL3504 there appeared to be expression in head hypodermis or muscle whereas in UL3501 expression was seen in the pharynx and some head neurons. UL3501 expresses slightly more strongly. |
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Expr1449
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PKC3 is expressed throughout the lifespan of C. elegans. However, both the content and intracellular distribution of PKC3 vary with development. For example, total PKC3 content in L1 larvae is increased 7-fold relative to the minimal level observed in L3 animals. Embryos and L4 larvae are also 3- to 4-fold enriched in PKC3. |
A very high proportion (75100%) of PKC3 partitions with the insoluble fraction of homogenates from embryos, L2L4 larvae, and young adult C. elegans. L1 larvae have the highest level of particulate PKC3 but also contain a similar amount of the kinase in cytosol. The highest level of soluble PKC3 is detected in L1 larvae. |
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Expr9288
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Significant expression of C10F3.4 seemed to be mostly confined to neurons and parts of the reproductive system. In addition, expression was observed in the spematheca and in anterior hypodermal cells. The high neuronal expression was found in all the normal larval stages observed. C10F3.4 expression was also detected in developing embryos. |
Cytosolic expression of the C10F3.4 protein was detected throughout the neuronal system. |
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Expr13568
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ztf-6::gfp expression is first observed in late embryogenesis (threefold stage). In early larval stages, the ztf-6::gfp-expressing cells include head hypodermal cells, head muscle cells, neurons, and ectodermal blast cells along the body (all P and all V cells) and in the tail. Starting in the L2 stage, additional neurons in P cell-derived ventral cord motor neurons express ztf-6::gfp. Because of the postdeirid loss phenotype of ztf-6 mutants, we examined the postdeirid lineage in more detail. We observe ztf-6 expression in the V5 cell of freshly hatched L1 animals. Upon division of the V5 cell into a posterior and anterior daughter, we observe expression in both the anterior and posterior daughters of the V5 cell. The descendent of the posterior V5.p daughter, V5.pa (the founder of the postdeirid lineage), and V5.pp also continue to express ztf-6::gfp. Within the V5.pa lineage, expression of ztf-6::gfp is retained in the blast cells that generate the glial cells and the PDE and PVD neurons (v5.paaa, V5.papa, V5.paap, V5.papp). No expression is detected at later stages in this lineage. |
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Operon: CEOP4344 |
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Expr9496
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Expressed in anterior neurons (not individually identified in this study) from L2 to adult, pharynx from L2 to adult, mid body cell bodies at L4, head hypodermal cells/muscle at adult stage. |
Sub-cellular localization within the body wall muscle: Nucleus and Cytoplasm/Other |
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Expr9734
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Although weaker, GFP expression looks similar to UL3218 (generated by the same strategy as UL3146 but with gfp inserted just before the fkh-9 stop codon). GFP was seen from the comma stage embryo. From the L1 stage, GFP was seen in neurons and hypodermis in the head, but the fluorescence signal was much brighter before hatching. |
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Other Strains: OH14272 |
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Expr14213
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Rectal epithelial cells, head and tail hypodermis, vulva, seam cells, PVT. There is some neuronal exp but it's variable and dim |
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Expr12167
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pcbd-1 was expressed in the epidermis (hyp7, tail and head epidermis) -similar to what observed in cat-4 transgenics- but was not highly expressed in 5HT and DA neurons. These transgenics all also showed some expression of varying intensity in other cells (non-epidermal cells, and non-5HT and non-DA neurons in the head and body). |
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Other strain-- UL844 |
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Expr163
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Expression is seen in the cells of the E-lineage from the 8E cell stage. Consistent with this expression is seen in all intestinal cells in larvae and adults. Some anterior expression in the head hypodermis and pharynx is also occasionally seen. |
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Expr169
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Weak expression is seen in the pharynx and cells of the anterior hypodermis from 3-fold embryo stage until adulthood. |
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Expr9769
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Widespread GFP expression. GFP was observed in many nuclei in the head, both inside and outside of the pharynx, in different cell types probably including neurons, hypodermis and muscle. In the anterior half of head the GFP showed a diffuse distribution over most of the pharynx as if poorly localized to the nucleus. The GFP becomes tightly nuclear-localized more posteriorly with expression in body wall muscle, intestine, spermathecae and hypodermis, and several neuronal cells in the tail. |
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Expr10010
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ALG-1 was prominently expressed in the pharynx. Cells in the tail also displayed specific expression. Expression was seen in vulva, seam cells, ventral nerve chord and somatic gonad. The endogenous ALG-1 expression was confirmed for the pharynx and head neurons with a polyclonal antibody raised against the ALG-1 specific N-terminus region. Examination of the ALG-1 expression during larval development did not reveal differences in expression during the four larval stages and adults. During embryogenesis ALG-1 is first detected at the beginning of the morphogenetic phase. |
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Legacy Data: Author "Lynch AS" "Bauer PK" "Hope IA"Date 1996-06. |
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Expr63
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The E lineage exhibits expression from the 2 to 8 E cell stages of development. This component is highly mosaic in the present strains. Adulthood sees anterior members of the syncytial hypoderm showing expression. |
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Expr1164
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Transgenic animals that carry this construct show GFP expression in a variety of tissue types. GFP expression is observed in the intestine, and the posterior cells express GFP more intensely than the remaining intestinal cells. Other cells include the rectal epithelial cells, the pharynx, the somatic gonad, and vulval hypodermal cells. In addition, the IP3 receptor is expressed in hypodermal cells of the tail, rectum, and head. Pharyngeal expression is restricted to the muscles of the metacorpus, isthmus, and the anterior portion of the terminal bulb (m4, m5, and m6). This construct was only expressed in neurons LUA and PDA. GFP is expressed in the gonad sheath cells, spermatheca, spermathecal valve, and uterine sheath cells. GFP is expressed in the vulval hypodermal cells. |
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Expr12601
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sox-2 expression is restricted to subsets of neuroblasts. sox-2 is expressed relatively late in nervous system development, in the progenitor of differentiated neurons, but not in earlier neuroectodermal cells. sox-2 is also expressed in some progenitors of non-neural tissue, in the head hypodermis and the arcade cells. sox-2 is expressed in several postembryonic blast cells that are generated in the embryo. These blast cells include the B, Y, F, U and K rectal epithelial cells and the seam cells along the body. Although expression of sox-2 is absent in the terminal neurons generated by these lineages, sox-2 expression extends beyond the blast cell stage [e.g. sox-2 expression is maintained in the V5 daughters (V5a and V5p) but is lost in the next division].sox-2 is expressed in the sensory neurons AWB, AWC, IL1, IL2, URA, URB, OLL, the interneurons AIM, AIN, AVK, RIH and the motor neuron class RME. The sox-2 fosmid reporter or sox-2 smFISH did not show any expression in the germ line or oocytes of young adult worms. Expression patterns obtained by smFISH were very similar to the ones observed with fosmid reporters. |
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Expr12440
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sox-2::gfp was expressed in the nucleus of both AWC and AWB neurons. The expression of sox-2 in AWC and AWB is maintained throughout life. sox-2 is also continuously expressed in other sensory neurons (IL1, IL2, URA, URB, OLL), interneurons (AIM, AIN, AVK, RIH), and motor neurons (RME), as well as in other tissues such as head hypodermis, arcade cells, and rectal epithelial cells. |
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Other Strain: OH14759 |
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Expr14171
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ASK, head hypodermis |
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Expr15880
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Examination of an additional independently generated raga-1 line showed a similar pattern, but with higher adult levels of hypodermal expression (especially in the head), and in the entire somatic gonad including the spermatheca and gonadal sheath cells. |
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Expr9742
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GFP expression was seen from at least the bean stage of the embryo, through all stages to adulthood. GFP was present in anterior hypodermis, as well as in ventral nerve cord neurons and in the intestine and maybe head muscle. From the L3, GFP expression was also seen in the distal tip cell. |
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Expr9791
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Broad GFP expression was seen from early embryogenesis to the adult, but particularly prominently in the intestine and many neurons in the head and tail. There was also higher GFP levels in the seam cells in the L3/L4, possibly at the time of moulting. GFP was observed in the body wall and head, possibly in the hypodermis, in larvae and the adult, and in the adult spermathecae and vulva or uterus. |
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Expr10011
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ALG-2 was predominantly expressed in subsets of neurons in the head ganglia. Cells in the tail also displayed specific expression. Expression was seen in vulva, seam cells, ventral nerve chord and somatic gonad. Examination of the ALG-2 expression during larval development did not reveal differences in expression during the four larval stages and adults. During embryogenesis ALG-2 started to be expressed from pre-morphogenetic embryonic stages. |
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Expr1200134
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Data from the TransgeneOme project |
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Expr16312
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FLN-2 is expressed in the hypodermis including the main body syncytium hyp7 and the smaller head and tail hypodermal cells. In hyp7, FLN-2::GFP shows both vesicular and parallel stripe-like patterns. FLN-2::GFP co-localizes with VHA-5::RFP on the vesicular structures. The parallel stripe- like pattern of FLN-2 overlaps with VAB-10A, a component of the epidermal attachment structures called fibrous organelles (FOs), which suggests that FLN-2 also localizes to the FOs (Zhang and Labouesse, 2010). FLN-2 is also expressed in the kidney-like excretory canal cell, which is important for osmoregulation (Liegeois et al., 2006; Sundaram and Buechner, 2016). In the excretory canal, FLN-2::GFP is aligned at the apical side of VHA-5::RFP. |
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[aqp-8p::aqp-8::gfp] and [aqp-8p::aqp-8::mCherry] translational fusions. aqp-8 full-length genomic DNA with a 1.0 kilobase (kb) upstream sequence was amplified by PCR and cloned into the GFP vector pPD95.75 by Sph1 and BamH1. The amplified mCherry DNA was ligated to the 3' end of the aqp-8 full-length genomic DNA by BamH1-KpnI into pPD95.75. [aqp-8p::gfp] transcriptional fusion. |
Expr10636
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The aqp-8p::gfp-derived transcriptional GFP reporter exhibits uniform labeling of the excretory canal cytoplasm, whereas aqp-8p::aqp-8::gfp- derived translational AQP-8::GFP fusion proteins identify the subcellular canalicular compartment. AQP-8 is also expressed outside the excretory system. Low levels of AQP-8 can be detected in several other tissues, such as coelomocytes, a posterior intestinal cell pair (INT 9), rectum, vulval muscles, male germline and ray, hypodermic or mesodermal cell near the pharynx, pharynx muscle. |
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Expr16445
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Expression of the bus-22 RFP marker was detectable in seam cells, the excretory duct cell, and head hypodermal cells. |
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Assayed in C. elegans. |
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Expr11950
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Expression was found consistently in hypodermal cells of all lines examined. GFP expression was particularly prominent in the hypodermal cell nuclei hyp5, hyp6, and hyp7 of the head and in the pair of hyp7 cell nuclei in the tail of adult worms. Using sensitive staining techniques for the detection of b-galactosidase activity, expression was also detected in larval stages, again strongly in the head and tail regions. The lacZ expression was less pronounced in the hypodermal cells of the body, with expression being detected in the hypodermal cell nuclei hyp4, hyp5, hyp6, and hyp7 of the head. With prolonged staining (overnight at room temperature) lacZ expression was also observed in the vulval cells of the adult, which are of hypodermal origin, in the adult body wall muscle cells, and in embryonic stages. |
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Expr12711
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ptr-6 is expressed in the hypodermis, especially strongly in the head region. |
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