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Expr15649
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Expr15558
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15589
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Expr15591
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Expr15598
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Expr15604
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Expr15608
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Expr15611
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Promoter 4 |
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Expr15393
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All of the reporter constructs produced the same cell-specific expression pattern as transgenes. |
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Expr1438
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The reporter transgenes express ubiquitously in the early embryo starting at about the 100 cell stage during gastrulation. In late embryogenesis and posthatching, expression is more limited. Strongest expression is observed in migrating cells and growing neurons as these cells undergo movements on the epidermis. At hatching, the reporters express in many neurons throughout the animal, in several cells of the pharynx including some pharyngeal neurons, in the elongated processes of the excretory cells, in the amphid and phasmid sheath and socket cells, in the tail hypodermis, and at later stages in intestine, muscles, vulva, and somatic gonad including the gonad sheath and hermaphrodite distal tip cells. The neurons expressing unc-73 include the PLM, ALM, PDE, HSN, CAN, PHC, and PVN neurons and the ventral cord motorneurons. Expression in the HSNs is absent in early larval stages, but begins late in the second larval stage (L2), precisely when axon outgrowth is initiated from the HSN cell bodies. The Q neuroblasts, Pn neuroectoblasts, sex myoblasts (SMs), and canal associated neurons (CANs) express unc-73 reporters. The left and right Q cells begin to express the GFP reporter as they initiate their migrations along the longitudinal axis of the epidermis during the early first larval (L1) stage, and expression in these cells continues beyond the completion of their first division. The unc-73 reporters express in the Pn cells just before this second phase of movemen. The distal tip cells also express the unc-73/reporters during their migration. |
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Expr15402
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Clone: pUL#JRH/AH7 |
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Expr7632
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Expression is seen in 2 nerve cells in the head and 5 in the tail. The expression is very specific, late embryo to adult. Expressing nerve cells may be AVG, M5, PVT and 4 of PVCL/R, PVNL/R, PVQL/R. |
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Expr15564
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Reporter gene fusion type not specified. late embryo(author) = fully-elongated embryo (wjc) |
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Expr1056
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GFP expression in the anterior was observed in the sensory neurons AFDL/R, in the interneurons BDUL/R, and in the asymmetrical interneuron ALA. The expression pattern of the GFP constructs was continuous from the late embryonic through all larval stages and maintained in adults. Both constructs show the same expression pattern. Larval and adult expression of CEH-14 protein in AFDL/R and BDUL/R was confirmed by immunolocalization using the CEH-14-specific antiserum. One additional cell expressing CEH-14 was observed in the head, at a location consistent with that for ALA. The staining was always nuclear. In the tail region, ceh-14 is expressed in PVT, PVQL/R, DVC, PVNL/R, PVWL/R, PVR, PHCL/R, PHAL/R, and PHBL/R. |
Always expressed in nuclei. |
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Expr15648
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Expr10986
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ceh-14 expression starts around the 280 min stage mainly in the anterior part of the embryo. In later stages (comma stage), the expression is seen in more cells in the head, but also in some cells in the tail. Judged by position and shape, most of them are neuronal precursors, although we have not precisely identified them. From larval to adult stages, neuronal expression is observed in the ALA interneuron and a pair of sensory neurons, AFDL/R, in the head, and in the BDUL/R interneurons in the anterior body (see also Cassata et al., 2000a). In the tail, we see expression in the sensory- and interneurons PHAL/R, PHBL/R, PHCL/R, DVC, PVCL/R, PVNL/R, PVQL/R, PVT, PVWL/R and PVR. The neuronal expression in the head and tail is confirmed by an anti-CEH-14 rabbit antiserum. We also note expression in the six touch neurons ALML/R, AVM, PVM and PLML/R, although it is seen only in strains with integrated reporter constructs. In addition to the neuronal expression, ceh-14 expression is also seen in the hypodermis and spermatheca as previously described (Kagoshima et al., 2000). |
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Expr15626
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Expr12717
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Expr15612
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Expr15326
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Expr14654
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The male-specific neuronal expression of lin-29a is not observed in male-specific neurons but is, interestingly, entirely restricted to sex-shared neurons (i.e. neurons that are generated in both sexes). Intriguingly, most of these neurons had not been previously reported to show sexually dimorphic gene expression patterns or functions. Specifically, 21 out of 116 sex-shared neuron classes express lin-29a, including seven sensory neuron classes in the head and tail (AWA, ASG, ASJ, ASK, ADF, PHB, PLM), seven interneuron classes (AVA, RIA, AIM, AVG, RIF, PVC, PVN), a few head and tail motor neurons (SAB, PDA, PDB) and most of the sex-shared ventral nerve cord motor neurons (dorsal and ventral A, B and D-type and AS neurons). Expression of lin-29a in all these neurons in the male nervous system is precisely temporally controlled; it is first observed in the early L4 stage and persists throughout adulthood. |
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Expr15315
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Expr15316
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acr-5 ORF |
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Expr12722
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