WormMine: ExpressionPattern Expr3765

• Pattern: To examine lim-4 expression during the embryogenesis, the eggs were stained at various developing stages with anti-myc antibodies. A GFP reporter was used for the GATA gene elt-2, which is specifically expressed in the gut lineage, to stage the embryos. MYC::LIM-4 immunoreactivity is first detected in two cells approximately 160 min post-fertilization at 8 gut nuclei stage. The two MYC::LIM-4-staining cells was identified as the neuroblasts ABalpppppa and ABpraaappa that respectively give rise to left/right ADF and AWB neurons, based on triple labeling of the embryos with antibodies raised against the LIN-26 protein, which is expressed in the nuclei of the hypodermal cells that border the ADF/AWB mother cells. Like lim-4::gfp, the MYC::LIM-4 immunoreactivity is not detected in ADF at any stage of larvae or adults, but it is observed in AWB and several other neurons in the head throughout life (N > 200). It is difficult to determine if LIM-4 is excluded from ADF or it is present there transiently.
• Primary Identifier: Expr3765
• Remark: This myc::lim-4 transgene potently rescues ADF serotonergic phenotype in yz12 mutants, indicating that the MYC::LIM-4 protein is expressed in cells necessary to specify ADF serotonergic identity.

4 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBR	lineage name: ABpraaappap	WBbt:0003828
Amphid wing cells, neurons having ciliated sheet-like sensory endings closely associated with amphid sheath	AWBL	lineage name: ABalpppppap	WBbt:0003829
embryonic cell	ABpraaappa		WBbt:0006483
embryonic cell	ABalpppppa		WBbt:0006638

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002987	lim-4	ZC64.4	Caenorhabditis elegans

0 Life Stages