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Expr15649
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Expr15442
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Expr15558
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Expr15567
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15586
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Expr15651
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Expr15652
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Expr15589
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Expr15591
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Expr15598
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr963
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Transgenic animals bearing pFX1G1 had high levels of GFP fluorescence or immunoreactivity in embryonic and postembryonic neurons. fax-1::gfp expression was first detected in embryos prior to elongation (approximately 350 minutes of development). By approximately 400 minutes, there is strong fax-1::gfp expression in as many as 20 neurons in the embryonic head and 1-2 neurons in the embryonic tail. fax-1::gfp is expressed in 20 neurons postembryonically, through the adult stage. The position of these neurons indicates that most or all of them are among the 22 neurons that express fax-1::gfp embryonically. These cells include both AVKR and AVKL. fax-1::gfp was not observed in either of the HSN or PVQ neurons, or in the PVPR neuron at any stage of development. fax-1::gfp expression was observed in several other neurons and two non-neuronal cell types in transgenic animals carrying pFX1G1. These include the pairs of CEPD and URX sensory neurons, three pharyngeal neurons (M1, MI and probably M5), two pairs of ring interneurons (including the RIC pair), five neurons in the retrovesicular ganglion (including SABD and the pair of SABV neurons), a single neuron in the preanal ganglion (either PVPL or PVT) and a single neuron in the dorsorectal ganglion of the tail (probably DVA). There is incompletely penetrant fax-1::gfp expression in a few additional neurons that were not identified, and in the non neuronal dorsal rectal cell and distal tip cells of the somatic gonad. |
GFP immunoreactivity was present in the cytoplasm, axons and nuclei of cells. Axons of neurons that express fax-1::gfp embryonically were observed in the process of outgrowth. |
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Expr15611
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Expr13958
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egl-44 expression was much more restricted then zag-1, being found only in the FLP, ADL, and SAB neurons, as well as a few VA and VB motor neurons. Moreover, egl-44 was widely expressed in hypodermis, pharynx, and intestine, whereas zag-1 expression was absent in these tissues. |
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Expr14024
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Several head neurons, I1 or I2 pharyngeal neurons, head mesodermal cell?, pharynx, vulva, CAN, VC, DA9, 1 neuron pair in the tail, PVT, another cell in PAG? |
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Picture: Fig 3. |
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Expr8676
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Expression in the alimentary canal: Strong and consistent expression in pm1, pm3. Weak or rare expression in pharyngeal epithelium, pm2, pm4, pm5, pm6, pm7, pm8, intestine, rectal epithelial cells. Expression in the nervous system: ILso, AIN, AVF, AVJ, AVK, PVR, SAB. Expression in the reproductive system: In adult stage, expressed in Gonad sheath, Vulva(low), vulval muscle, uterine muscle. In developing larva stage, expressed in vulval muscle, uterine muscle. inx-8 is expressed broadly, albeit at very low levels, around two-fold stage, and its expression becomes stronger in the pharynx, nervous tissue, HMC, and GLR cells as development continues. In the reproductive system, expression of inx-8 starts during early larval stages and continues during migrations of the great granddaughters of the SM blast cell to their final locations and after the sex muscles achieve their final structures. inx-8 is expressed in the hypodermal cells of the animal in postembryonic stages. |
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Neuronal gene expression pattern from collation by Shawn Lockery of neuron-specific promotors posted 20/04/98 (http://chinook.uoregon.edu). Since cell AS is listed under 'body' in the pattern, it presumably means the AS.1-11 ventral cord neurons rather than the AS amphid neurons.[sdm-curator] |
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Expr320
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head: IL2 URA URB SAA SAB SIA SIB SMB SMD RMD AIY, sev more; phar: M1 M2 M5 I1f I6f, others; body: VA VB VC DA DB AS SDQ HSNf; tail: ALN PLN others [J. Duerr (personal communication to Shawn Lockery)], antibody |
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[ser-2::gfp] transcriptional fusion constructs. Ser-2 reporter constructs were generated by using a PCR fusion protocol, using pPD95.75 as a template for green fluorescent protein (gfp). For all gfp fusion primers listed, gfp vector sequence is indicated in lowercase, and gene-specific sequence is indicated in uppercase. [ser-2::gfp] translational fusion. A translational fusion of the whole ser-2 locus to gfp was created by using an in vivo recombination technique. Specifically, two overlapping PCR fragments, one containing the 5' part of a locus, the other containing the remainder of the locus PCR-fused to gfp, were coinjected into the worm. Recombination of these two fragments via the homologous region leads to the expression of a full-length ser-2::gfp fusion. |
Expr2707
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Expression using the upstream regulatory regions of exon 1bc (ser-2prom2::gfp) is mostly restricted to the AIYL/R, AIZL/R, RID, DVA, BDUL/R, SIADL/R, and SIAVL/R interneurons. Less consistent expression is observed in PVT. In addition, expression is observed in the RMEL/R motor neurons. Outside the nervous system, expression can be observed in the excretory gland cells. No more transcriptional regulatory information is contained within intronic regions by generating a fusion of gfp to the full coding genomic ser-2 locus using an in vivo recombination technique([ser-2::gfp] translational fusion. Transgenic animals expressing such a construct show an expression pattern similar to the one observed with the ser-2prom1::gfp construct. The upstream regulatory region of the third splice form, containing exon 1d(ser-2prom3::gfp), drives expression exclusively in two sensory neuron classes, OLL(L/R) and PVD(L/R). ser-2prom1::gfp is expressed in the AIY interneuron class and a set of unidentified neurons. These neurons were identified as head and tail interneuron classes, namely AVHL/R, AUAL/R, AIYL/R, RICL/R, SABVL/R, RID, RIAL/R, SABD, SDQ, CANL/R, DA9, LUAL/R, ALNL/R, and PVCL/R. In addition to its expression in neurons, ser-2prom1::gfp is also expressed in pharyngeal cells (NSM neurons and pm1/6 muscles) and in head muscles. In males, expression can be observed in posterior dorsal and ventral body wall muscles, the male-specific diagonal muscles, and several posterior neurons likely to be CP neurons. |
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Expr15570
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Expr15644
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Expr15648
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Expr13161
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Expr13162
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Expr3012
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Expressed in AUA, BAG, DA, DD, DVB, LUA, PHC, PVC, SAB, URX, VD, uv1, head muscle. Faint or variable expression observed in socket cells. Male specific expression in Ray 4. |
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