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Picture: Fig. S1B. |
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Expr9002
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GFP fluorescence was observed in most, if not all, cells, including the T, V5.pa, Z1/Z4 and Q cells, and all of their descendants. |
The BET-1::GFP signal exhibited a granular distribution in the nuclei at interphase. During division, BET-1::GFP signals were observed along the metaphase plate, indicating that BET-1 colocalized with the chromosomes at mitosis. |
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Don't dump for LEGO CAM (capturing function in GO) |
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Expr12818
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TCER-1::GFP was visible at all stages of embryonic and larval development. In adults, we observed strong nuclear localization of TCER-1::GFP in intestinal cells, many head and body neurons, muscle and hypodermal cells. In some intestinal cells, weak expression was also observed in the cytoplasm. |
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Expr11922
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gcn-1 was expressed in most cells during all stages of development. gcn-1 expression was observed in head neurons, hypodermal cells, intestinal cells, body wall muscles, and pharyngeal neurons, including the M4 neuron. |
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Expr13334
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In hermaphrodites and males, selt-1.1 GFP expression was observed in neurons, epithelial and muscle cells of transgenic animals carrying both transcriptional (i.e. selt-1.2 promoter driving GFP expression) and translational (i.e. containing selt-1.2 promoter, exons and introns driving GFP expression) constructs. Crosses with the pan-neuronal marker rab-3 fused to the red fluorescent protein (RFP) in the nuclei confirmed that SELT-1.1 is expressed in all neurons of the nervous system. The expression of selt-1.1 in the ADL, ASH, ASI, ASJ, ASK, AWB amphid sensilla neurons was also confirmed by DiI staining. In the epithelia, selt-1.1 is expressed in the hypodermal, arcade, pharyngeal, vulval and rectal cells. selt-1.1 was not found to be expressed in the intestine or in the gonad. Muscle cells expressing selt-1.1 include the somatic muscle cells from head, neck and body wall as well as the non-striated pharyngeal muscles. SELT-1.1 expression was observed throughout development, from pre-bean embryonic stages to the adult stage. Embryos expressed GFP in most cells, also with a perinuclear localization. |
The translational Pselt-1.1::selt-1.1::gfp reporter revealed perinuclear localization, consistent with the ER localization previously reported for mammalian SELENOT. The ER localization of SELT-1.1 was confirmed by expressing into the QW1266[Pselt- 1.1::selt-1.1::gfp] the ER marker tram-1 fused to mcherry in muscle cells. |
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Expr14889
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hrpk-1::gfp is ubiquitously expressed during all C. elegans developmental stages. The C-terminally tagged hrpk-1::gfp expression is observed in the gut, muscle, neuronal, and hypodermal tissues, where it localizes to the cell nuclei. |
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Expr14937
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The endogenous VWA-8::GFP was expressed in mitochondria of hypodermis, intestine and muscle, but was not detectable in neurons. In hypodermis, VWA-8::GFP was colocalized with MitoTracker Red, a mitochondria marker. |
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Expr14947
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MICU-1::GFP was detected at low levels in germ cells, epidermis, and some muscles. MICU-1::GFP was detected at low levels in PLM neuron soma, colocalizing with a mitochondrial marker, but was below the limit of detection in axons. |
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Expr15523
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pry-1 expression revealed expression in almost all tissues during development. Expression in seam cells, neuronal cells, muscles, hypodermis, and intestine was readily visible. The most enriched tissues include neurons and muscles. A closer examination of GFP localization in developing animals revealed bright fluorescence in the ventral cord region, which includes neuronal and non-neuronal cells. The expression was largely similar in adults, although the fluorescence was much higher in BWMs. The posterior end of the intestine, near the rectal opening, showed a strong signal in L4 and adult animals; however, the rest of the intestine lacked a detectable expression. |
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Expr12923
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A rescuing MML-1::GFP translational reporter was widely expressed and found in the cytoplasm and nuclei of the intestine, neurons, muscle, hypodermis, excretory cell and other tissues. A closer examination of the subcellular localization revealed that MML-1::GFP also co-localized with the mitochondria, comparable to mammalian MondoA. |
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Expr12480
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GFP fluorescence in animals carrying the unc-23::gfp construct is found throughout development appearing first at the 1.5-fold stage of embryogenesis in a few unidentified cells. Expression of the GFP-tagged UNC-23 protein continues during embryogenesis and by the 3-fold stage, it has expanded to include muscle, hypodermal and pharyngeal cells. In adult hermaphrodites GFP fluorescence is expressed in the pharynx, body wall muscle cells, hypodermis, vulva, and H cells, as well as some unidentified neurons and touch cells. In the hypodermis, UNC-23::GFP is distributed throughout the entire tissue including some nuclei and nucleoli, and in addition, is localized in a pattern reminiscent of the intermediate filaments. In body wall muscle cells, UNC-23::GFP is localized to the dense bodies and M lines in a pattern similar to that observed for several proteins including PAT-3/b-integrin, UNC-112, UNC-97/ PINCH, and PAT-4/ILK. |
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Expr13545
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Two neurons and several additional muscle and hypodermal cells. |
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Expr13546
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Few motor neurons in addition to many other muscle and hypodermal cells. |
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Expr14586
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PFD-6::GFP fusion protein was localized in both the cytosol and nuclei of intestinal, hypodermal, muscle, and neuronal cells throughout the developmental stages. |
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Expr13556
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cam-1 is broadly expressed in many cell types, including muscle, hypodermis, and neurons (Forrester et al. 1999), making direct identification of relevant cells difficult. To determine whether cam-1 is expressed in PVP and PVQ neurons, the Pcam-1::cam-1::GFP strain was crossed with hdIs26[Podr-2::CFP; Psra-6::dsRed2], which expresses CFP in PVP and dsRed in PVQ. Strong CAM-1 expression was observed in PVQ but only weak expression in PVP. CAM-1 is also expressed in PVT. |
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Expr9995
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vha-12 is broadly expressed in most, if not all, somatic cell-types in larvae and adults. Robust GFP expression is observed in the H-shaped excretory cell, the excretory pore, intestine, and hypodermal cells. GFP reporter expression is observed at low levels in muscle. Consistent with the uncoordinated phenotype, expression is observed in all neurons. |
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Expr12088
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Transgenic animals harboring GFP under the control of the asp-6 promoter show fluorescence in various tissues at the gravid-adult stage, while expression is not detectable during earlier developmental stages. Expressing tissues include the intestine, muscle cells, pharynx and hypodermal cells. |
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Expr13240
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In adults the pattern of UNC-44C::GFP(ju1413) fluorescence was generally consistent with that described for UNC-44 common isoforms by immunostaining (Otsuka et al., 2002); however we also observed strong expression in muscles and in gonadal spermathecal/sheath cells. UNC-44C::GFP(ju1413) expression was detected from early embryos to late larvae. Immediately before the bean stage of embryogenesis, UNC- 44C::GFP proteins were expressed in epidermal cells, within which they localized to the cell periphery. At postnatal L1 stage, UNC-44C::GFP was evident at the peripheries of epidermal seam cells and gut cells. In late larvae and adults, UNC-44C::GFP was seen in cells undergoing morphogenesis or fusion, such as vulval and seam cells. |
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Expr11177
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The expression dynamics of mom-2/Wnt are quite different throughout the worm life span. Expression of mom-2/Wnt increases 3 fold during the first 5 days of adulthood and then decreases 4 fold by day 8 of adulthood, eventually showing little or no expression in old worms. Expression localization of mom-2 differs slightly between aging and development. During development, mom-2 is expressed throughout the whole body of the worm, in muscles, hypodermal and intestinal cells, vulva precursor cells, as well as in ventral cord motor neurons (Gleason et al. 2006). In young (day1 and 2) and middle-aged (day 5) adults, mom-2/Wnt expression was observed only in posterior intestinal and intestinal rectal valve cells. The authors were not able to detect any mom-2 expression in any other tissues. |
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Expr14276
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The strn-1::GFP reporter was highly expressed throughout the nervous system, but was also expressed at lower levels in at least some other cell types, including some hypodermal and muscle cells. |
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[Psax-7::sax-7::gfp::sl2::mCherry] translational fusion. |
Expr11329
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The mCherry signal revealed that SAX-7 was expressed in the hypodermal cells and in many neurons, but not in PVD. Expression and localization of SAX-7 was observed early in development during the L1 and early L2 stages, long before the formation of PVD branches. |
Within the hypodermal cell, SAX-7::GFP showed specific localization to two sublateral stripes and the hypodermal-seam cell junctions. |
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Expr13899
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GFP signal from the transcriptional fusion Prib-1::gfp fills the cytoplasm of expressing cells, whereas the VENUS signal displays a punctate cytoplasmic pattern in cells expressing the translational fusion Prib-1::rib-1::venus, consistent with RIB-1 being localized to the Golgi apparatus. Moreover, both the transcriptional and translational fusions have a very similar spatial and temporal expression pattern during development: expression was visible in neurons, hypodermal cells, muscles of the digestive system, and reproductive tissues. |
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Expr13900
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GFP signal from the transcriptional fusion Prib-1::gfp fills the cytoplasm of expressing cells, whereas the VENUS signal displays a punctate cytoplasmic pattern in cells expressing the translational fusion Prib-1::rib-1::venus, consistent with RIB-1 being localized to the Golgi apparatus. Moreover, both the transcriptional and translational fusions have a very similar spatial and temporal expression pattern during development: expression was visible in neurons, hypodermal cells, muscles of the digestive system, and reproductive tissues. |
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Expr12014
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A daf-31 promoter::GFP reporter gene indicates daf-31 is expressed in multiple tissues including neurons, pharynx, intestine and hypodermal cells from L1 to the adult stages. |
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Expr12076
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OGT-1::GFP showed that OGT-1 was diffusely localized and was widely expressed throughout development, including in the intestine, hypodermal cells and neurons. |
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Expr12110
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NEKL-3::GFP expression initiated in the hypodermis of embryos coincident with the onset of morphogenesis and increased during the remainder of embryogenesis. In larvae and adults, NEKL-3::GFP was observed in hyp7 and other hypodermal cells but not in seam cells. In hyp7, NEKL-3::GFP was present in small spheres or puncta and small tubules that were almost exclusively in the extreme apical region and apparently beneath the plasma membrane. Although not particularly mobile, some movement of the puncta was observed. In most worms, GFP was also present in hyp7 at its boundary with the seam cells. NEKL-3::GFP was also observed in the vulva and in certain undefined neurons that make contact with body muscles and in the uterus |
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Expr12148
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mtm-6 is expressed in many neurons throughout the worm, the pre-anal ganglion, hypodermal cells, the anal depressor muscle, and additional non-neuronal of cells in the tail. The expression pattern is fairly constant through development. |
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Expr1200380
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Data from the TransgeneOme project |
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Expr14436
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edc-3 is broadly expressed during embryogenesis. Its expression is maintained in several cell types, including pharyngeal muscles, intestinal cells, neurons, and hypodermic cells, at early post-embryonic stages. |
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Expr14626
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Promoter reporter assays confirmed that sax-7 was expressed in the hypodermal cells and the neurons, including ALA and PVD. |
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TSP-14B isoform expression. |
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Expr16023
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We found that TSP-14A and TSP-14B exhibit distinct expression and localization patterns. First, only TSP-14A, but not TSP-14B, is detectable in the germline and sperm cells, as well as at the tip of the anterior sensory cilia, while TSP-14B is detectable in the pharynx. Both TSP-14A and TSP-14B are found in hypodermal cellsand the developing vulva. However, the two isoforms exhibit different subcellular localization patterns. In hypodermal cells, TSP-14A is primarily localized in intracellular vesicles, while TSP-14B is mainly localized on the cell surface. In the developing vulva, TSP-14A is localized on the apical side, while TSP-14B is localized on the basolateral membrane. Similar localization patterns hold true in the pharynx. |
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