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No detailed description on expression pattern in other life stages.. Picture: Fig. 1G, 1H. |
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Expr8874
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This reporter was expressed at low levels in the early somatic gonadal lineage. Prominent expression was first seen in Z1.pa and Z4.ap, and their descendants. In the L3 stage, the F12E12.5 reporter was expressed in ventral and dorsal uterine precursors. Reporter expression was not seen in early embryos, perhaps due to the limited sequences used to generate the reporter. |
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Expression pattern at adult stage was not described. |
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Expr1410
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Expression in Z1 and Z4, the somatic gonad precursors, starts a few hours after these are born. Up to the formation of the somatic gonad primordium in late L2, LIN-26 protein was detected in all cells of the somatic gonad, except in the distal tip cells or dtcs (Z1.aa and Z4.pp). Staining was strongest in Z1 and Z4 and became weaker after each cell division. However, after Z1.a and Z4.p divided, LIN-26 was initially not detected in any of their daughters, but resynthesis occurred in Z1.ap and Z4.pa. During the L3 stage, expression was only detected in the anchor cell (AC) until the Pn.pxx cells were generated. After the last division round in L4 larvae, all uterine nuclei and two spermathecal-uterine junction nuclei expressed LIN-26 at very low levels. |
nuclei |
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Expr3090
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An hlh-2::lacZ transcriptional reporter appears to be expressed in a similar pattern to HLH-2 protein during the L1 stage. HLH-2 does not accumulate detectably in any cell of the gonad until Z1 and Z4 have undergone two rounds of division, near the end of the L1 stage. HLH-2 was consistently observed in the DTCs (Z1.aa and Z4.pp) and in the presumptive AC and mature AC. HLH-2 was also weakly and inconsistently present in Z1.pp and Z4.aa (the parents of Z1.ppp and Z4.aaa), as well as their sisters, Z1.pa and Z4.ap. |
While HLH-2 accumulation in the DTCs is nuclear, as expected for a transcription factor, HLH-2 is detectable in the cytoplasm as well as the nucleus of other gonadal cells. |
