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Isoform 1b.1 |
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Expr11755
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Isoform 1b.2 |
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Expr11756
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Expr15558
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Expr9325
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Synaptogyrin is expressed in all 26 GABAergic neurons including also RMER and most though not all other neurons. Synaptogyrin is absent in amphids and phasmids and can be detected in non-neuronal glial-like sheath cells in adult worms. The cephalic neurons CEPDR/L and CEPVR/L and amphid-associated sheath cells CEPshDR/L, CEPshVR/L were tentatively positive. Several other neurons that could be tentatively identified in the anterior part are MI, M4, I4, AVL, AIY, RIS, I5, M3R/L, and in the posterior part DVA, AS11, ALNR/L, DVC, DVB, PQR, DA9 (characteristic axonal process denoted by arrowhead), VD13, DD6, VD12. Of these, AVL, RIS, VD13, DD6 and VD12 are GABAergic based on the colocalization with the unc-47p::GFP reporter. In addition, IL neurons were tentatively identified in the anterior (IL*). Synaptogyrin reporter constructs are also expressed in developing neurons. The expression of sng-1p::YFP is closely associated with the development of the nervous system being absent in the gastrula stage with first fluorescence in neuronal precursor cells and newly-formed neurons in the anterior part during the 1.5-fold stage. In addition, it is also detected transiently in cells in the posterior body at the 1.25-fold and 1.5-fold stage. |
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The cellular expression pattern for nlp-3 fragments was indistinguishable from the cellular expression pattern for nlp-3 subcloned constructs. Transgenic lines were generated by coinjection of the nlp::gfp construct (5070 ng/ul) and lin-15 rescue construct (pJM24,100 ng/ul) into lin-15(n765ts) animals. At least two independent transgenic lines were analyzed for each nlp gene; 5-10 animals were scored per line. 1,1'-Dioctadecyl-3,3,3',3 -tetramethylindodicarbocyanine perchlorate (Molecular Probes) was used to stain amphid and phasmid neurons to facilitate identification. |
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Expr1688
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Expressed in ADF, ASE, ASH, AWB, ASJ, BAG, HSN, I1, I2, I3, I4, MI, M3, NSMR, 3 head neurons, VNC, oocytes, I6, M2, pm1VL, intestine. |
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Expr15571
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Expr15572
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Expr15573
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Expr15579
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Expr15583
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Expr15586
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Expr15651
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Expr15652
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Expr15589
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Expr13158
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Expr15591
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Expr15598
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Expr11622
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Expression of ceh-34::gfp transgene began during embryogenesis. CEH-34::GFP was localized to the nuclei of expressing cells. During embryonic morphogenesis and larval development and throughout adulthood, expression of the ceh-34::gfp transgene was seen predominantly in pharyngeal cells. The ceh-34::gfp transgene was expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2, I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2) and pharyngeal epithelial cells (e1 and e3), and some body wall muscles around the anterior pharynx. |
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Expr15604
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Expr14590
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Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. |
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Expr15608
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Expr15611
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Expr3759
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ser-7::gfp DNA injected into C. elegans at 0.5-0.05 ng/Ul produced lines that exhibited marked GFP fluorescence in a number of pharyngeal neurons, including the MCs, M4, I2s, I3, M5 and occasionally in the M3s, I4, I6 and M2s. Strong GFP fluorescence was also observed in the vulval muscles. |
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Expr3136
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C33G8.5 showed medium to strong expression in scattered nonchemosensory neurons.5 |
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Reporter gene fusion type not specified. The antibody staining showed EAT-20 expression in the seam cells and hypodermal cells, which is inconsistent with the absence of GFP expression in those cells in the promoter trap lines, suggesting that p5E5 may not contain all the cis-regulatory elements necessary for the expression of the eat-20 gene. |
GFP expression was analyzed in ncIs1 hermaphrodites carrying p5E5 as a stable integrated transgenic array. Though p5E5 contains a 2-kb genomic fragment derived from LGV at the 5' side in addition to the fragment corresponding to the eat-20 gene, it is confirmed that an extrachromosomal array of p5E5 and that of p5E5del, in which the 2-kb fragment was deleted, gave the same GFP expression pattern. Thus, the fragment at the 5' part of the insert had no effects on the expression of GFP. Neurons expressing GFP were first identified in L1 larvae. |
Expr976
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In embryos, GFP expression was first detected at the 2-fold stage. Sometimes eight cells in the anterior half of the body expressed GFP. At the 3-fold stage, several cells around the pharynx expressed GFP in most embryos. About half of the embryos also expressed GFP weakly in the pharynx. At L1, GFP was detected in a subset of neurons. About half of the larvae also expressed GFP in the pharynx; expression in the terminal bulb was the most intense. At the adult stage, GFP was detected in the pharyngeal muscles, m3, m4, and m6. In addition, GFP was detected in a subset of neurons: IL1, OLQ, BAG, and ALN, several circumpharyngeal cells, and coelomocytes. Very faint GFP fluorescence was detected in the pharyngeal neurons including I4 and I5 in L1 larvae. |
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Expr11834
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Expression from the pPHA2::GFP-F construct was restricted to three types of pharyngeal cells: reproducible expression in the pharyngeal interneuron I4, and weak and rare (detected in <10% of transgenic worms) expression in pm5 muscle cells and in three pharyngeal epithelial cells. |
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Expr11836
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When the 2.7-kb promoter is present to drive a truncated pha-2 gene containing only the first three exons and first two introns, the complete pharyngeal expression profile of the full-length rescue-competent pPHA2::GFP-A construct is restored (pPHA2::GFP-E). This construct also supports expression in rectal gland cells, but shows no expression in the head neurons or intestine. This shows that important regulatory sequences exist within the first two introns and that these elements operate in concert with 5' sequences that are in the 500 to 2700 bp to drive robust expression of pha-2 in the pm5, I4, metacorpus cells, and pharyngeal epithelial cells. |
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Expr11837
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The pPHA2::GFP-D construct, which contains the third intron as well as part of the fourth exon, exhibited an expression profile similar to the pPHA2::GFP-E construct (Expr11836). |
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Expr12198
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Examination of the expression pattern of prdx-2 using a rescuing translational reporter (prdx-2prom::prdx-2::mCherry) revealed expression in a broad set of tissues: I2, I4, and intestine (as previously reported; Isermann et al., 2004), as well as muscle (pharyngeal muscle 1, vulval muscle, body wall muscle), epithelial cells (e1, e3), and many neurons in the head and tail. |
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Expr15570
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