1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00017641 | csr-1 | F20D12.1 | Caenorhabditis elegans |
| Primary Identifier | Expr8822 | Subcellular Localization | Finally, all aspects of the localization patterns were absent in each respective mutant background. In the mitotic cells of embryos, each factor became enriched in prophase nuclei. As chromosomes condensed, DRH-3, EGO-1, and EKL-1 became enriched along the length of each chromosome, while CSR-1 remained nuclear. All four proteins exhibited robust localization around the metaphase plate. CSR-1 and DRH-3 displayed a pattern similar to cohesins, whereas EKL-1 (and to a lesser degree, EGO-1) appeared to be more closely associated with chromosomes in a pattern similar to kinetochore proteins. In fact, EKL-1 retained a robust association with chromosomes during anaphase, whereas the other RNAi factors became more difficult to detect. Cytoplasmic localization was also detected for each protein. The larger CSR-1 isoform was expressed throughout larval development and was also present at low levels in post-mitotic populations lacking a germline. Quantitative real-time RT-PCR analysis of both csr-1 transcripts indicated that their expression recapitulates the protein expression pattern. DRH-3, EGO-1, and CSR-1 colocalize in the germline with PGL-1, a previously characterized component of the germline nuage structures called P granules. EKL-1 was not detected in P granules. While many developmentally important factors transiently localize to P granules, DRH-3 and CSR-1 maintained their P granule localization in germ cells throughout the life cycle. As oocytes matured, EGO-1 was lost from the P granules, while DRH-3 and CSR-1 maintained P granule association. In mature oocytes, CSR-1 and EGO-1 both became enriched in nuclei, where CSR-1 was enriched on the diakinetic chromosomes. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00017641 | csr-1 | F20D12.1 | Caenorhabditis elegans |
