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Expr11704
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Expression of the transcriptional fusion Ptop-1::gfp was not detected in early embryonic cells due to maternal germline silencing of the multi-copy transgenic gene. The GFP expression, however, was observed in most cells at the late embryonic stage and decreased with larval development. In the larval stages, GFP expression was predominantly present in the neuronal system, including sensory neurons (ILso, URX, RIC, IL1/IL2, AIY/AIM and RIG/RIF), motor neurons (VC4, VC5, HSN, PVD and PVM) of hermaphrodites and tail neurons (SPD and SPV/SPC) of males. The expression of the transcriptional fusion Ptop-1::gfp was strong in DTCs during the L3-L4 stage when gonad migration proceeds. |
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Expr12716
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Expr12717
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Expr11374
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PVR (50%), PVQ (58%) PVW (58%), ADL (96%), ADF (72%), SMD (96%), RME (66%), AIA (64%), ASH (60%), AVG (56%), RIF (44%), PVS/PVU (17%), SPC (39%), oblique muscles (53%), intestine (90%). Percentages correspond to the fraction of animals examined that expressed GFP in the indicated neurons. Above 50% marked as certain, below as uncertain. |
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Expr2455
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unc-103::GFP construct expressed broadly in the head and ventral cord neurons in both males and hermaphrodites. In the male tail the SPC motor neurons and the PCB postcloacal sensory neurons expressed the construct. Many neurons express the construct, but because of variability in cell position and cellular localization of the fusion protein in processes, the list of unc-103-expressing cells is far from complete. In the head region ALA, ADL, ASK, AVH, AVJ, AIN, AVA, ASJ, SMDD, SIA, ADE, and AVD express the construct. In the tail region PHA, DVC, ALN, and PVP express the construct. The unc-103 fusion protein accumulates extensively in the processes of many ventral cord neurons; however, only some cells in the AS class of excitatory neurons was identified. In the adult hermaphrodite HSN expresses the unc-103::gfp construct. |
The fusion protein aggregates mostly on the cytoplasmic membrane of neuronal processes and in many, but not all, cases of cell bodies. In larval hermaphrodites and males some of the cells had cell body localization of UNC-103::GFP. |
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Expr950
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Early in development, 1497pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites. Early in development, pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites. |
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Picture: Fig. 3C,D. |
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Expr8296
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Expressed in the SPC motor neurons, PCB and PCA postcloacal sensilla (p.c.s.) neurons, male spicule protractor muscles and anal depressor muscle, VD and DD ventral cord neurons, some tail and nerve ring neurons, and body wall muscles. In hermaphrodites, YFP expression is in the same set of cells, except those specific to males. |
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Expr10821
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unc-63::YFP was expressed in the body wall muscles, the sexually dimorphic anal depressor muscle and sphincter muscle, and every male-specific sex muscle, including the spicule protractor and retractor muscles, the diagonal muscles, the gubernacular muscles, and the oblique muscles. It was also expressed in the SPC neurons. |
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Expr10822
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unc-38::YFP was expressed in the body wall muscles, the sexually dimorphic anal depressor muscle and sphincter muscle, and every male-specific sex muscle, including the spicule protractor and retractor muscles, the diagonal muscles, the gubernacular muscles, and the oblique muscles. It was also expressed in the SPC neurons. |
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Expr9999
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A 2.8 kb promoter of cysl-1 drove GFP expression mainly in the nervous system of adult hermaphrodites. The cysl-1::GFP expression pattern was similar for the transcriptional and translational reporters. GFP was observed in subsets of pharyngeal neurons, amphid sensory neurons and tail neurons, starting from late embryonic stages and persisting into adults. We identified GFP-positive cells as the AVM sensory neuron, the BDU interneurons, and the pharyngeal I1 interneurons and M2 motor neurons, based on their characteristic processes and nuclear positions. GFP in body wall muscles, hypoderm, and intestine was present in larvae but only weakly detectable in adult animals. |
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Expr15030
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In addition to male sex muscles, EGL-2::YFP is also expressed on the cell bodies, neural processes, and sensory endings of the male ray, post-cloacal, and spicule sensory neurons. the protractor muscles' EGL-2::YFP levels also increased between 24 and 48 h in wild-type males. |
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Expr12313
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In these transgenic males, UNC-7 expression was found, in addition to the sex muscles (Expr12312), in several mating circuit neurons (SPC, PCA, PCB, HOA, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1). |
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Expr10584
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The prig-6RIG-6::GFP construct drives expression of all RIG-6 isoforms in fusion with GFP, under their endogenous promoter. rig-6 expression is detected during embryogenesis and is maintained throughout adulthood. rig-6 is expressed in I1 and I3 pharyngeal interneurons, NSM motor neurons and other unidentified head neurons. RIG-6::GFP is also localized in the cell bodies and axons of different subtypes of VNC motorneurons, in the HSN and the CAN neurons. Neuronal expression of rig-6 also includes the ALM and PLM touch receptor neurons. Non-neuronal cells expressing rig-6 include somatic muscles and the hypodermis. We also detected rig-6 expression in the developing and adult EC. The prig-6RIG-6::GFP construct drives expression of all RIG-6 isoforms in fusion with GFP, under their endogenous promoter. rig-6 expression is detected during embryogenesis and is maintained throughout adulthood. rig-6 is expressed in I1 and I3 pharyngeal interneurons, NSM motor neurons and other unidentified head neurons. RIG-6::GFP is also localized in the cell bodies and axons of different subtypes of VNC motorneurons, in the HSN and the CAN neurons. Neuronal expression of rig-6 also includes the ALM and PLM touch receptor neurons. Non-neuronal cells expressing rig-6 include somatic muscles and the hypodermis. We also detected rig-6 expression in the developing and adult EC. |
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Expr12408
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With a construct including 8 kb upstream of the first exon and the entire unc-7 genomic region fused to YFP, UNC-7 expression was found, in addition to the sex muscles reported in Expr12407, in several mating circuit neurons (SPC, PCA, PCB, HOA, HOB, and unidentified ray neurons). Similar to previous studies (Starich et al., 2009; Yeh et al., 2009; Kawano et al., 2011), this reporter also expresses, in head ganglion neurons, several ventral chord neurons, and tail neurons of the hermaphrodite, in addition to few unreported pharyngeal neurons (I5, M2, I4, M3, and I1). |
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Only celluar expression patterns in the mail tale was reported. No detail on expression pattern in other tissues. --wjc. |
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Expr1818
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PCB, PCC, and SPC neurons expressed an unc-17::gfp construct beginning at mid-L4 and continuing throughout adulthood. |
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