WormMine: AnatomyTerm WBbt:0005792

• Definition: any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.
• Name: intestinal cell
• Primary Identifier: WBbt:0005792

34 Children

Definition	Name	Synonym	Primary Identifier
Intestinal cell of the seventh intestinal ring, right side.	int7R embryonic	lineage name: Eprpa	WBbt:0004344
Intestinal cell of the sixth intestinal ring, left side.	int6L embryonic	lineage name: Eplap	WBbt:0004350
Intestinal cell of the seventh intestinal ring, left side.	int7L embryonic	lineage name: Eplpa	WBbt:0004349
Intestinal cell of the fourth intestinal ring, dorsal side.	int4D embryonic	lineage name: Epraa	WBbt:0004346
Intestinal cell of the sixth intestinal ring, right side.	int6R embryonic	lineage name: Eprap	WBbt:0004345
Intestinal cell of the third intestinal ring, dorsal side.	int3D embryonic	lineage name: Earap	WBbt:0004354
Intestinal cell	int2D	lineage name: Earpa	WBbt:0004353
Intestinal cell of the fifth intestinal ring, right side.	int5R embryonic	lineage name: Earpp	WBbt:0004352
Intestinal cell of the fouthth intestinal ring, ventral side.	int4V embryonic	lineage name: Eplaa	WBbt:0004351
Intestinal cell of the third intestinal ring, ventral side.	int3V embryonic	lineage name: Ealap	WBbt:0004359
Intestinal cell	int2V	lineage name: Ealpa	WBbt:0004358
Intestinal cell of the fifth intestinal ring, left side.	int5L embryonic	lineage name: Ealpp	WBbt:0004357
Intestinal cell	int1DR	lineage name: Earaad	WBbt:0004356
Intestinal cell	int1VR	lineage name: Earaav	WBbt:0004355
Intestinal cell	int1DL	lineage name: Ealaad	WBbt:0004361
Intestinal cell	int1VL	lineage name: Ealaav	WBbt:0004360
Intestinal cell of the nineth intestinal ring, right side.	int9R embryonic	lineage name: Eprppp	WBbt:0004342
Intestinal cell of the eighth intestinal ring, right side.	int8R embryonic	lineage name: Eprppa	WBbt:0004343
Intestinal cell of the nineth intestinal ring, left side.	int9L embryonic	lineage name: Eplppp	WBbt:0004347
Intestinal cell of the eighth intestinal ring, left side.	int8L embryonic	lineage name: Eplppa	WBbt:0004348
Post-embryonic, binucleated int8R.	int8R post-embryonic		WBbt:0008236
Post-embryonic, binucleated int8L.	int8L post-embryonic		WBbt:0008235
Post-embryonic, binucleated int9R.	int9R post-embryonic		WBbt:0008238
Post-embryonic, binucleated int9L.	int9L post-embryonic		WBbt:0008237
Post-embryonic, binucleated int6R.	int6R post-embryonic		WBbt:0008232
Post-embryonic, binucleated int4D.	int4D post-embryonic		WBbt:0008227
Post-embryonic, binucleated int7R.	int7R post-embryonic		WBbt:0008234
Post-embryonic, binucleated int7L.	int7L post-embryonic		WBbt:0008233
Post-embryonic, binucleated int5R.	int5R post-embryonic		WBbt:0008230
Post-embryonic, binucleated int6L.	int6L post-embryonic		WBbt:0008231

4 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 3_4 expressed in intestine.	scVI 0.6.0	WBPaper00065841:3_4
	Single-cell RNA-Seq cell group 3_5 expressed in intestine.	scVI 0.6.0	WBPaper00065841:3_5
	Single-cell RNA-Seq cell group 3_1 expressed in intestine.	scVI 0.6.0	WBPaper00065841:3_1
	Single-cell RNA-Seq cell group 3_2 expressed in intestine.	scVI 0.6.0	WBPaper00065841:3_2

225 Expression Patterns

• Primary Identifier: Expr16078
• Pattern: In adult hermaphrodites, CBR-CSR-1 was enriched in both germline and somatic nuclei. We observed CBR-CSR-1 in association with oocyte nuclei and enriched on chromosomes in oocytes. Again, we did not reliably observe CSR-1 localization to perinuclear and cytoplasmic granules, which would be indicative of P granules. CBR-CSR-1 was also enriched in somatic gut nuclei, further supporting a nuclear role for this Argonaute.

• Primary Identifier: Expr16507
• Subcellular Localization: To examine where PALS-17 and PALS-20 proteins are expressed and if they colocalize in intestinal cells, we examined PALS-17::GFP and PALS-20::wrmScarlet translational reporters co-expressed in the same animals. We found that these two proteins formed puncta in a subset of intestinal cells, and in most cases, they colocalized with each other. Furthermore, we observed fluorescent signals from both reporters at the apical border of the intestinal cells, lining the intestinal lumen. PALS-17 and PALS-20 were also diffusely expressed in the cytosol in some intestinal cells.

• Primary Identifier: Expr16506
• Subcellular Localization: To examine where PALS-17 and PALS-20 proteins are expressed and if they colocalize in intestinal cells, we examined PALS-17::GFP and PALS-20::wrmScarlet translational reporters co-expressed in the same animals. We found that these two proteins formed puncta in a subset of intestinal cells, and in most cases, they colocalized with each other. Furthermore, we observed fluorescent signals from both reporters at the apical border of the intestinal cells, lining the intestinal lumen. PALS-17 and PALS-20 were also diffusely expressed in the cytosol in some intestinal cells.

• Primary Identifier: Expr10799
• Pattern: The expression pattern of nlp-40 with a functional, endogenous nlp-40 promoter used to drive the expression of GFP with a nuclear localization sequence (NLS) revealed that nlp-40 was exclusively expressed in intestinal cells.

• Primary Identifier: Expr10800
• Subcellular Localization: NLP-40::YFP fusion protein adopted a highly punctate pattern of fluorescence on the basolateral surface of the intestinal cells. YFP fluorescence was also detected in six coelomocytes.

• Primary Identifier: Expr9985
• Pattern: GFP expression was first observed in the intestinal cells of gastrula-stage embryos and continued throughout all larval and adult stages. In transgenic male worms expressing the Pdaao-1::GFP construct, no significant difference in expression or localization patterns was apparent compared to hermaphrodites.

• Primary Identifier: Expr13443
• Pattern: In transgenic worms, hrpu-2::GFP signal was observed in a variety of cell types, including motor neurons and many other neurons (such as nerve ring neurons and ventral nerve cord neurons), body-wall muscle cells, pharyngeal muscle cells, and intestinal cells. In determining the colocalization of HRPU-2 and SLO-2, we observed that both transgenes are expressed in body-wall muscle cells and many neurons, including the VA5 and VD5 motor neurons used for electrophysiological recordings, although their expression patterns differ in some tissues such as pharyngeal muscles.

• Primary Identifier: Expr12818
• Remark: Don't dump for LEGO CAM (capturing function in GO)
• Pattern: TCER-1::GFP was visible at all stages of embryonic and larval development. In adults, we observed strong nuclear localization of TCER-1::GFP in intestinal cells, many head and body neurons, muscle and hypodermal cells. In some intestinal cells, weak expression was also observed in the cytoplasm.

• Primary Identifier: Expr11907
• Pattern: ICD-2::GFP was mainly expressed in intestinal cells and muscle cells.
• Subcellular Localization: ICD-2 colocalized with the ER marker TRAM-1::mCherry.

• Primary Identifier: Expr12594
• Pattern: Using a translational vrp-1::gfp reporter construct, expression was observed in the intestinal nuclei of both adult and larval hermaphrodites.

• Primary Identifier: Expr11922
• Pattern: gcn-1 was expressed in most cells during all stages of development. gcn-1 expression was observed in head neurons, hypodermal cells, intestinal cells, body wall muscles, and pharyngeal neurons, including the M4 neuron.

• Primary Identifier: Expr12640
• Pattern: W09B7.1 is expressed in the intestinal nucleus.

• Primary Identifier: Expr12641
• Pattern: K05F6.7 is expressed in the intestine.

• Primary Identifier: Expr12636
• Pattern: W09B7.2::GFP was mainly expressed in the intestinal cytoplasm.

• Primary Identifier: Expr12643
• Pattern: W06A11.4 is expressed in the intestine.

• Primary Identifier: Expr11753
• Remark: genomic

• Primary Identifier: Expr11756
• Remark: Isoform 1b.2

• Primary Identifier: Expr12830
• Reporter Gene: fosmid fUL#SB103
• Pattern: GFP, arising from the R11A8.5::gfp fusion, was observed in the cytoplasm of the muscle and intestinal cells only.

• Primary Identifier: Expr15244

• Primary Identifier: Expr15249

• Primary Identifier: Expr12161
• Pattern: Transgenic worms containing the smf-3 pro::gfp construct demonstrated strong GFP expression at all stages of development, beginning as early as the comma stage embryo and continuing through larval and adult stages. Expression was spatially confined to intestinal cells, excretory cells, vulval epithelial cells, and neuronal cells. Fluorescence was largely observed at the apical ends of the adult and larval intestines; in neuronal cells, particularly in the head neurons and hypodermis; in H-shaped excretory cells; and in vulval epithelial cells. Worms expressing the smf-3 pro::gfp construct exhibited distinct intestinal expression, which encompassed most of the intestine.

• Primary Identifier: Expr15253

• Primary Identifier: Expr2964
• Remark: In situ RNA hybridization is consistent with this organ-specific expression pattern, and additionally detects early embryonic erm-1 RNA.
• Pattern: Expressed in embryos, larvae, and adults. ERM-1-GFP is enriched along the luminal surfaces of intestinal, excretory canal, and gonadal tubular epithelia, but not in the remaining organ lumina of the pharynx, rectum, and excretory pore cell, which all secrete cuticle.
• Subcellular Localization: Enriched along the luminal surfaces.

• Primary Identifier: Expr15228

• Primary Identifier: Expr15230

• Primary Identifier: Expr15231

• Primary Identifier: Expr15232
• Pattern: Unc-64 isoform A and B.

• Primary Identifier: Expr13326
• Pattern: SMG-2::GFP was expressed in the cytoplasm of cells, consistent with the finding that NMD functions in the cytoplasm. Tissues that expressed the smg-2::gfp transgene included neurons, intestine and muscles.

• Primary Identifier: Expr14889
• Pattern: hrpk-1::gfp is ubiquitously expressed during all C. elegans developmental stages. The C-terminally tagged hrpk-1::gfp expression is observed in the gut, muscle, neuronal, and hypodermal tissues, where it localizes to the cell nuclei.

• Primary Identifier: Expr12831
• Reporter Gene: fosmid fUL#SB103
• Pattern: SIR-2.1::mCherry driven by fUL#SB103 was observed, as for the singly recombineered fosmid, in the nerve cells in the head, the ventral nerve cord and the tail, in the hypodermis, in muscle cells and in cells of the intestine.
• Subcellular Localization: In all cell types the mCherry fluorescence was throughout the cytoplasm, but also nuclear-enriched.

0 Life Stages

2 Parents

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
a cellular object that consists of subcellular components, expresses genes or functions.	Cell	Cell type	WBbt:0004017