WormMine: ExpressionPattern Expr16348

• Pattern: mNG::LET-60 was present at high levels in VPCs during VPC induction in the L3 larval stage, where it localized predominantly to the intracellular region-cytosol and possibly endomembranes. We also observed that LET-60 levels were significantly higher in the 1° VPCs compared to the 2° and 3° VPCs during VPC induction where LET-60/Ras activity is higher (Shin & Reiner, 2018). Interestingly, mNG::LET-60 Ras was present at low levels in the uterine anchor cell and during early stages of VPC induction was largely found in the anchor cell nucleus. High levels of mNG::LET-60 were observed in the sex myoblast cells in the intracellular region and nucleus during the L3 and L4 larval stages (Figure 1F), where LET-60/Ras regulates sex myoblast migration and differentiation (Sundaram et al., 1996; Sundaram, 2013). High levels of mNG::LET-60 was also observed in tissues where LET-60/Ras is not known to function, such as nuclear and occasional nucleolar localization of mNG::LET-60 in intestinal cells in early larval stages, regionalized intracellular localization in the pharynx, and high levels of intracellular mNG::LET-60 accumulation in the spermatheca. Enriched intracellular mNG::LET-60 was detected in neurons associated with the nerve ring, consistent with let-60 mRNA expression and LET-60 function in olfactory neurons whose cell bodies and axons are located within the nerve ring (Hirotsu et al., 2000; Menini et al., 2010; Taylor et al., 2021). Finally, plasma membrane localization of mNG::LET-60 was found throughout the germline, in body wall muscle cells, and hypodermal seam cells, which are tissues where LET-60/Ras has known functions (Sundaram, 2013). We noted high levels of intracellular and nuclear mNG::LET-60 in the somatic sheath cells of the gonad, where LET-60 might regulate germline morphogenesis (Lu et al., 2008).
• Primary Identifier: Expr16348

11 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
	pharyngeal cell		WBbt:0005460
five pairs of thin gonadal sheath cells form a single layer covering the germ line component of each arm, each pair occupying a stereotyped position along the gonad proximal-distal axis.	gonadal sheath cell		WBbt:0005828
anchor cell, induces vulva, part of hermaphrodite gonad.	anchor cell	AC	WBbt:0004522
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809
Blast cell which divides to product sex-specific muscle cells.	sex myoblast		WBbt:0008373
any of 20 large epithelial cells which form a tube and are mostly situated as bilaterally symmetric pairs around the tubular lumen. Each of these cell pairs forms an intestinal ring ( II-IX int rings). The most anterior intestinal ring (int ring I), however, is made of four cells. Intestinal cells contain large nuclei with large nucleoli and numerous autofluorescent granules in their cytoplasm.	intestinal cell		WBbt:0005792

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002335	let-60	ZK792.6	Caenorhabditis elegans

3 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035