GFP expression was observed from the post-comma stage embryo to the mature adult. Very strong GFP expression was seen in the DVA neuron and 1 to 3 neurons in the head throughout development, including the adult. GFP seen in some other tail neurons in some individuals and in the ventral nerve cord, was particularly apparent in L3s and L4s. GFP was observed in the intestine from the L2 or L3 stage onwards. GFP was also seen in the developing hermaphrodite reproductive system, possibly in the uterus and vulva, and in adult vulval muscles. Diffuse fluorescence (presumably due to localization in the cytoplasm and nucleus) was apparent in the body wall, in a row of cells along both sides which increased in number with age and were presumably seam cells. GFP fluorescence was fainter in UL3931. The GFP expression pattern looks very similar, if not identical, to that observed for UL4083, which was generated by the same strategy as for UL3935 but using a different starting fosmid.
Primary Identifier
Expr9797
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9 Anatomy Terms
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A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.
The C. elegans life stage spanning 520-620min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and tripple fold. A stage between 2-fold embryo and fully-elongated embryo. Also called pretzel embryo or pretzel stage.
The C. elegans life stage spanning 420-460min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and fold back 50%. A stage between comma embryo and 2-fold embryo.
The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.
The C. elegans life stage spanning 460-520min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo is elongated and double fold. A stage between 1.5-fold embryo and 3-fold embryo.