WormMine: ExpressionPattern Expr10699

• Pattern: let-7 primary transcripts are first detected at the end of the first larval stage and exhibit pulses of expression near the molt of each larval stage. Both of the plet-7::GFP constructs drove temporally regulated expression, initially observed at the end of the first larval stage (L1). Starting in late L1 (~10 h at 25°C), robust GFP was seen in muscle and neuronal cells in the head, including the nerve ring. Midway through L2, GFP accumulated in the neurons and muscle cells of the tail, body wall muscle (BWM), and ventral nerve cord (VNC). Beginning in L3, all transgenic lines began to express GFP in vulva precursor cells. After the onset of GFP expression in a particular tissue, it was maintained until adults became gravid with embryos (~52 h at 25° C). GFP was not detected in the germ line. Although plet-7A::GFP and plet-7B::GFP transgenics showed similar spatio-temporal expression patterns in most cell types, they were not entirely overlapping. In hypodermal and seam cells, the plet-7B::GFP reporter produced a robust GFP signal at the L3 stage. However, plet-7A::GFP animals exhibited only weak, transient expression of GFP in the hypodermal cells beginning at the L3 stage. Early in the second stage of development, all intestinal cells showed high expression of GFP in plet-7B::GFP transgenic animals, while GFP was undetectable in the intestine of plet-7A::GFP transgenic animals.
• Primary Identifier: Expr10699
• Remark: plet-7A::GFP and plet-7B::GFP expression differs in intestine, hypodermal, and seam cells.

10 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772
Epidermal layer.	hypodermis	epidermis	WBbt:0005733
a large process bundle that runs along the vental mid-line extending from the ventral region of the nerve ring.	ventral nerve cord	ventral cord	WBbt:0005829
Longitudinal bands of muscle cells surrounding animal body, with one band running in each quadrant of the body, regulated contraction and relaxation of these muscles cause locomotion.	body wall musculature	body muscle	WBbt:0005813
neuron with its cell body situated in the head, excluding the pharynx.	head neuron		WBbt:0006751
body wall muscle in the head, including first eight cells of each muscle quadrants which are innervated by nerve ring neurons.	head muscle	head body wall muscle	WBbt:0006761
a group of hypodermal cells that lie along the apical midline of the hypodermis, at the extreme left and right sides between nose and tail	seam cell	lateral hypodermis	WBbt:0005753
the most extensive region of neuropil in the animal, consists of a large toroidal bundle of processes.	nerve ring	circumpharyngeal nerve ring	WBbt:0006749
posterior region, from rectum to the end	tail		WBbt:0005741
cell that has the potential to form structural part of a vulva.	vulval precursor cell	VPC	WBbt:0007809

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002285	let-7	C05G5.6	Caenorhabditis elegans

5 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035