WormMine: ExpressionPattern Expr3677

• Pattern: GFP fluorescence was observed throughout the entire intestine of transgenic animals expressing this nrf-5::gfp fusion. No GFP expression was observed in any other tissue. Furthermore, the nrf-5::gfp fusion rescued the Nrf and Peg phenotypes of nrf-5 mutants.
• Primary Identifier: Expr3677
• Subcellular Localization: nrf-5::gfp fluorescence was concentrated at the basal membrane of the intestine. Based on its similarity to secreted lipid binding proteins and the fact that it contains an N-terminal signal sequence, NRF-5 probably is secreted. The concentration of nrf-5::gfp fluorescence at the basal membrane of the intestine suggests that NRF-5 protein is secreted from the intestine into the pseudocoelomic fluid. GFP fluorescence was not observed in the pseudocoelomic fluid itself, however one possibility is that the level of secreted nrf-5::gfp is too low to detect in this manner, particularly against the background of fluorescence from the intestine.

1 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
A chain of very large cuboidal cells forming a wide central lumen in which food arrives from the posterior pharynx, is digested, and from which waste products proceed to the rectum. Intestinal rings form in groups of two and four cells surrounding the common lumen; thus the epithelium is only one cell deep at any point, with neighboring cells firmly secured to their neighbors by apical adherens junctions. These cells have very large nuclei and many large vacuoles, yolk granules, and other inclusions; the latter increase in number and electron density as the animal ages.	intestine	gut	WBbt:0005772

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003812	nrf-5	F55B12.5	Caenorhabditis elegans

0 Life Stages