WormMine: ExpressionPattern Expr2632

• Pattern: During later stages of embryogenesis, KLP-18 levels gradually diminished in somatic cells but increased in the primordial germ cells Z2 and Z3, presumably due to expression in the embryonic germ line. The germ line stained brightly for KLP-18 at all stages of larval development. Adult hermaphrodites contained high levels of cytoplasmic stain in the distal gonad arm. Adults also showed high levels of staining in the proximal gonad arm.
• Primary Identifier: Expr2632
• Remark: A dramatic reduction of staining in embryos after preincubation of the antibody with an excess of KLP-18 fusion protein. Staining was also greatly reduced or eliminated in embryos subjected to RNAi depletion of KLP-18.
• Subcellular Localization: During the meiotic divisions that occur in the male germ line, anti-KLP-18 staining patterns were similar to those seen in female meiosis and mitosis. KLP-18 was concentrated at the spindle poles, which have centrosomes, and in the anaphase interzone. There was also a significant amount of KLP-18 staining on male meiotic chromosomes. Immunostaining showed KLP-18 colocalized with the disorganized MTs during prometaphase. As the MTs became ordered into a parallel bipolar array, KLP-18 concentrated most at the poles but was also present between the poles and chromosomes. The concentration at poles persisted during metaphase and early anaphase, but then shifted to the interzone between the separating chromosomes during late anaphase/telophase. Similar KLP-18 staining patterns were observed during meiosis II. KLP-18 staining was most concentrated in mitotic spindles toward the poles. During anaphase, KLP-18 staining also concentrated between the separating groups of chromosomes, with a dim zone at the spindle equator. After cytokinesis, KLP-18 accumulated somewhat around the nucleus and at the cortex near sites of cell-cell contact. Adult hermaphrodites contained high levels of cytoplasmic stain in the distal gonad arm.

3 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
Germ line precursor cell	Z3	lineage name: P4.a	WBbt:0004575
Germ line precursor cell	Z2	lineage name: P4.p	WBbt:0004576

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002228	klp-18	C06G3.2	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch.		L2 larva Ce	WBls:0000027
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch.		L1 larva Ce	WBls:0000024
	The stage that begins when a C.elegans individual is fully-developed and has reached maturity.		adult Ce	WBls:0000041
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003