WormMine: ExpressionPattern Expr1674

• Pattern: In hermaphrodites, newly synthesized ife-1 mRNA was first detected in the germline of L3 stage larvae. At this stage germ cells in the distal region of the gonad are mitotically proliferating and those in the proximal region are entering meiosis. As germline development progressed, the hybridization signal intensified in the regions of the gonad undergoing spermatogenesis. The signal persisted after spermatogenesis was complete; a reduced level of ife-1 mRNA was detectable in adult germlines undergoing oogenesis. In embryos, the distribution of ife-1 mRNA was similar to a pattern described previously as class I maternal mRNAs. These mRNAs show a uniform distribution in all cells during early cleavage stages and disappear at later stages. ife-1 mRNA was detected in all cells in embryos until the 100-200-cell stage and gradually disappeared by the 300-400-cell stage.
• Primary Identifier: Expr1674
• Remark: 400-cell stage (author) = comma embryo (curator) --wjc.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
organ producing either sperm or ova.	gonad		WBbt:0005175

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002059	ife-1	F53A2.6	Caenorhabditis elegans

6 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch.		L4 larva Ce	WBls:0000038
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The C. elegans life stage spanning 0-350min after first cleavage at 20 Centigrade. Proliferate from 1 cell to 560 cells. From start of first cleavage until cleavage is over.		proliferating embryo Ce	WBls:0000004
	The C. elegans life stage spanning 390-420min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. The shape of embryo looks like a comma. A stage between bean embryo and 1.5-fold embryo.		comma embryo Ce	WBls:0000017
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057
	The C. elegans life stage spanning 350-390min after first cleavage at 20 Centigrade. Cell number remains at ~560 cells, with some new cells generated and some cells go through programmed cell death. Emrbyo elongation started but have not formed comma shape yet. The shape of embryo looks like a lima bean. A stage right before comma embryo. Also called lima embryo or lima bean stage.		bean embryo Ce	WBls:0000016