WormMine: ExpressionPattern Expr13464

• Pattern: ETR-1 is expressed in both the germline and the somatic gonadal tissue of the hermaphrodite gonad. A medial optical section through the hermaphrodite gonad shows that ETR-1 exhibits a diffuse, cytoplasmic staining throughout the entire hermaphrodite gonad. We also observed cytoplasmic expression of ETR-1 within both the female and male gametes (egg and sperm, respectively). A superficial optical section of the proximal hermaphrodite gonad shows that ETR-1 is localized specifically to the gonadal sheath cell myofilaments. This result was confirmed by colocalization with an antibody against myosin heavy chain A (MHCA) (Hubbard and Greenstein, 2000; Rose et al., 1997). Further confirmation of ETR-1 being expressed in the somatic gonadal cells emerges when imaging the surface of the distal gonad. ETR-1 exhibits a honeycomb pattern in the distal germ line that is consistent with the pattern observed by Hall et al. (1999) in their description of the individual somatic gonadal sheath cells via TEM and immunofluorescence studies (Hall et al., 1999). Analysis of embryos indicates early embryonic expression of ETR-1 that persists through the comma stage of embryonic development. In early embryos (1- to 16-cell stage), ETR-1 is diffusely cytoplasmic. In older embryos (>32-cell), while there still remains some diffuse cytoplasmic ETR-1 staining, a majority of the ETR-1 is now nuclear.
• Primary Identifier: Expr13464
• Subcellular Localization: In early embryos (1- to 16-cell stage), ETR-1 is diffusely cytoplasmic. In older embryos (>32-cell), while there still remains some diffuse cytoplasmic ETR-1 staining, a majority of the ETR-1 is now nuclear.

2 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
cell line which early in development becomes differentiated from the remaining somatic cell line, and alone has the potential to undergo meiosis and form gametes.	germ line	germline	WBbt:0005784
The components of the gonad that are separate from the germline proper. In hermaphrodite, these include five tissues which are all derived from the somatic primordium : the distal tip cells, the gonadal sheath, the spermatheca, the spermatheca-uterine valve (sp-ut) and the uterus.	somatic gonad	somatic germline	WBbt:0005785

1 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001340	etr-1	T01D1.2	Caenorhabditis elegans

1 Life Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003