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Expr1200068
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Data from the TransgeneOme project |
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Expr1200176
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Data from the TransgeneOme project |
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Expr13901
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Prib-1::gfp is broadly expressed in ectodermal and mesodermal cells during embryogenesis. A salient feature of the rib-1 expression pattern is that it is very dynamic in hypodermal cells during development. In embryogenesis, Prib-1::gfp is detected along the entire layer of hypodermoblasts that surrounds the gastrulating embryo at about 200 minutes after fertilization. By the early comma stage of embryogenesis, Prib-1::gfp is expressed at high levels in hypodermal cells of the elongating embryo, including hypodermal cells extending ventrally during ventral closure and in the two rows of dorsal hypodermal cells undergoing dorsal intercalation. Following these embryonic morphogenetic events, expression of Prib-1::gfp in the hypodermal cells of the body wall is no longer visible during larval and adult stages, except for seam cells undergoing fusion during larval development. Also, hypodermal cells of the developing vulva express Prib-1::gfp, at a low expression level in L3 larvae and at a stronger level in L4 larvae and just molted young adults, and vanishing in vulval cells in the adult. The nervous and digestive systems express Prib-1::gfp stably and continuously from embryogenesis throughout adulthood. Strong and sustained expression is seen in motorneurons, interneurons, sensory neurons (including AVM), neurons in the head and tail ganglia, with the GFP signal filling axons running along the ventral and dorsal nerve cords, commissures, and sublaterals. Expression in neurons of the ventral nerve cord and of the head ganglia is visible in 1.5-, 2-, and 3-fold embryos, and persists into adulthood. Strong expression of Prib-1::gfp is also observed in the pharynx from the 2-fold stage of embryogenesis onwards and remained strong in adults (procorpus, metacorpus, terminal bulb, grinder, and pharyngeal-intestinal valve). The anal depressor, the anal sphincter, the two enteric muscles, the spermathecae and the uterine muscles maintain expression in adults. |
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Expr977
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Neither immunoblotting nor immunostaining analysis with the anti-EAT-20 polyclonal antibody detected any signal in eat-20 mutants. At the 16-cell stage, patches of staining on the entire surface of embryos were first detected. From the comma stage on, staining was detected in coelomocytes, the nervous tissues, hypodermal cells, and the pharynx. At the comma stage, the apical surface of the alimentary canal was stained intensely and the basal surface of presumptive pharynx was moderately stained. The staining in the presumptive pharynx gradually weakened thereafter and disappeared completely at the late 3-fold stage. The staining of the apical surface of the pharynx and pharyngeal intestinal valve remained intense throughout the rest of the embryogenesis stage. Granular staining in the region surrounding the pharyngeal lumen was observed from the L2 stage on and spread to the external surface. Staining of the entire surface of the pharyngeal muscle was observed in the late L3 or L4 stage. The inner linings of the intestine and anus were intensely stained until the 2-fold stage, and then the staining became weak and disappeared completely at the 3-fold stage. The anterior-most intestinal cell became stained from the L3 stage. Staining was also observed in the nervous tissues. At the rostral end of the head, staining that seemed to correspond to the distal segments of labial process bundles was seen from the comma stage up to the adult stage. In larvae, the staining sometimes extended posteriorly to the level of the isthmus. A pair of cells, which might be support cells of sensory neurons posterior to the distal structures, were stained from the L1 stage up to the adult stage. The motor neurons in the ventral cord, which sometimes expressed GFP in the promoter trap line, were stained at the early L1 stage. This staining disappeared at the late L1 stage and, in the later stages, was replaced by segmental staining of the ventral nerve cord. The nerve ring and the nerve bundles that connect the nerve ring and the ventral nerve cord had dots of very faint staining. On the lateral body wall at the base of the tail spike, staining was detected from the L2 stage up to the adult stage, which may correspond to the axon of ALN neuron that expressed reporter GFP in the promoter trap lines. In the adult male tail, sensory rays were intensely stained. There was also extensive hypodermal staining. Weak staining of the seam cells began at the 3-fold stage and the staining became intense from the L3 stage up to the adult stage. Thin longitudinal bands were stained along the dorsal and ventral midline from the rostral end of the body to the base of the tail spike, which corresponds to the position of the dorsal and ventral hypodermal ridges. The hypodermal staining co-localizing with the position of the ventral nerve cord was the most intense. The hypodermal cells at the opening and inside of the vulva were stained from the L4 stage up to the adult stage. The hypodermis around the anus was stained from the L2 stage up to the adult stage. The coelomocytes were continuously stained from the comma stage up to the adult stage. |
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Expr1200069
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Data from the TransgeneOme project |
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Expr1200112
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Data from the TransgeneOme project |
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Expr1200225
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Data from the TransgeneOme project |
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Reporter gene fusion type not specified. |
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Expr2368
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GFP expression is restricted to the intestinal tract, indicating that cenadc2 gene is expressed in the intestinal tract. The expression pattern is evident from the early larva stage through the adult stage. The GFP fluorescence is detectable throughout the intestinal tract, starting from the pharynx all the way through the anus. The expression level of GFP is significantly greater in the anterior half of the intestine than in the posterior half. These experiments showed that the cenadc2 promoter-controlled YFP and the cenadc1 promoter-controlled CFP were coexpressed in the intestinal tract. This expression pattern was confirmed with at least 10 transgenic animals. |
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Expr11788
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cbp-1p::RFP expression was detected in most somatic cells, especially those that make up the alimentary system, confirming previous expression profiles. Analysis of dissected gonads revealed that the DTCs also expressed RFP in stages L2, L3, and L4 as well as in young adults, indicating that cbp-1 is expressed throughout DTC migration. |
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Reporter gene fusion type not specified. |
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Expr2367
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GFP expression is restricted to the intestinal tract, indicating that cenadc1 gene is expressed in the intestinal tract. The expression pattern is evident from the early larva stage through the adult stage. The GFP fluorescence is detectable throughout the intestinal tract, starting from the pharynx all the way through the anus. The expression level of GFP is significantly greater in the anterior half of the intestine than in the posterior half. |
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