WormMine: ExpressionPattern Expr3417

• Primary Identifier: Expr3417
• Subcellular Localization: In mated females, extracellular MSP exhibits a graded distribution, with a sharp boundary between the -1 and -2 oocytes. Fluorescence intensity measurements indicate that MSP is localized in a graded manner from the spermatheca to the oocyte. Fluorescence intensity measurements also indicate that there is significant MSP staining over the -2 and -3 oocytes. To pinpoint the localization of MSP at the oocyte cell surface, a 3D confocal analysis of MSP localization was conducted a in mated females using the RME-2 yolk receptor to mark the oocyte plasma membrane and the early endosomal compartments. Three-dimensional image reconstructions of the data indicate that MSP localizes in three regions: (1) in superficial focal planes at the oocyte cell surface with RME-2 just beneath; (2) in the same plane as the RME-2 signal; and (3) within the oocyte beneath the plasma membrane. Using anti-MSP mAbTR-20 and visual inspection, 91% of mated female gonad arms exhibited extracellular MSP localization (n=36), with 39% showing extracellular MSP as far as the most proximal oocyte, and the rest exhibiting extracellular MSP only within the spermatheca. With confocal microscopy, extracellular MSP appeared both punctate and diffuse in the spermatheca, the gonad arm and the uterus. Analysis of 3D data stacks indicated that punctate extracellular MSP was enriched near spermatozoa on the spermathecal walls. The largest puncta were at the diffraction limit of the microscope (0.5 um) and were found nearby spermatozoa. In the proximal gonad arm, MSP was more diffuse and localized in focal plane slices near the oocyte surface. In the uterus, large MSP puncta was observed close to spermatozoa. Diffuse MSP was also observed in extracellular spaces surrounding embryos in the uterus. MSP puncta can be observed near spermatozoa in the uterus and spermatheca using wide-field microscopy, when these regions were less crowded with spermatozoa.

6 Anatomy Terms

Definition	Name	Synonym	Primary Identifier
an accordion-like tube that contains sperm and is the site of oocyte fertilization.	spermatheca		WBbt:0005319
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
a female haploid germ cell.	oocyte	ovum	WBbt:0006797
Male haploid germ cell.	sperm	spermatozoa	WBbt:0006798
organ in hermaphrodite animal that produces both ova and sperm.	hermaphrodite gonad	ovotestis	WBbt:0005178
gonad of a male animal, produces sperm.	male gonad		WBbt:0006794

47 Genes

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003424	msp-3	F26G1.7	Caenorhabditis elegans
WBGene00003425	msp-10	K07F5.2	Caenorhabditis elegans
WBGene00003426	msp-19	F36H12.7	Caenorhabditis elegans
WBGene00003430	msp-32	R05F9.3	Caenorhabditis elegans
WBGene00003431	msp-33	R05F9.8	Caenorhabditis elegans
WBGene00003434	msp-38	K08F4.8	Caenorhabditis elegans
WBGene00003442	msp-49	C34F11.6	Caenorhabditis elegans
WBGene00003448	msp-55	C09B9.6	Caenorhabditis elegans
WBGene00003451	msp-58	ZK354.10	Caenorhabditis elegans
WBGene00003456	msp-63	K05F1.7	Caenorhabditis elegans
WBGene00003462	msp-74	F09C12.7	Caenorhabditis elegans
WBGene00003464	msp-77	F32B6.6	Caenorhabditis elegans
WBGene00003465	msp-78	T13F2.11	Caenorhabditis elegans
WBGene00003470	msp-152	ZK546.6	Caenorhabditis elegans
WBGene00003437	msp-42	F26G1.8	Caenorhabditis elegans
WBGene00003435	msp-40	C33F10.9	Caenorhabditis elegans
WBGene00003429	msp-31	R05F9.13	Caenorhabditis elegans
WBGene00003460	msp-71	F59A6.8	Caenorhabditis elegans
WBGene00003438	msp-45	F58A6.8	Caenorhabditis elegans
WBGene00003443	msp-50	C34F11.4	Caenorhabditis elegans
WBGene00003469	msp-142	K05F1.2	Caenorhabditis elegans
WBGene00003457	msp-64	ZK1248.6	Caenorhabditis elegans
WBGene00003450	msp-57	R13H9.2	Caenorhabditis elegans
WBGene00003446	msp-53	R13H9.4	Caenorhabditis elegans
WBGene00003445	msp-52	F36H12.6	Caenorhabditis elegans
WBGene00003444	msp-51	ZK354.5	Caenorhabditis elegans
WBGene00003468	msp-113	ZK354.4	Caenorhabditis elegans
WBGene00003452	msp-59	ZK354.11	Caenorhabditis elegans
WBGene00003458	msp-65	ZK354.1	Caenorhabditis elegans
WBGene00003463	msp-76	ZK1251.6	Caenorhabditis elegans

0 Life Stages