|
|
[ser-2::gfp] transcriptional fusion constructs. Ser-2 reporter constructs were generated by using a PCR fusion protocol, using pPD95.75 as a template for green fluorescent protein (gfp). For all gfp fusion primers listed, gfp vector sequence is indicated in lowercase, and gene-specific sequence is indicated in uppercase. [ser-2::gfp] translational fusion. A translational fusion of the whole ser-2 locus to gfp was created by using an in vivo recombination technique. Specifically, two overlapping PCR fragments, one containing the 5' part of a locus, the other containing the remainder of the locus PCR-fused to gfp, were coinjected into the worm. Recombination of these two fragments via the homologous region leads to the expression of a full-length ser-2::gfp fusion. |
Expr2707
|
Expression using the upstream regulatory regions of exon 1bc (ser-2prom2::gfp) is mostly restricted to the AIYL/R, AIZL/R, RID, DVA, BDUL/R, SIADL/R, and SIAVL/R interneurons. Less consistent expression is observed in PVT. In addition, expression is observed in the RMEL/R motor neurons. Outside the nervous system, expression can be observed in the excretory gland cells. No more transcriptional regulatory information is contained within intronic regions by generating a fusion of gfp to the full coding genomic ser-2 locus using an in vivo recombination technique([ser-2::gfp] translational fusion. Transgenic animals expressing such a construct show an expression pattern similar to the one observed with the ser-2prom1::gfp construct. The upstream regulatory region of the third splice form, containing exon 1d(ser-2prom3::gfp), drives expression exclusively in two sensory neuron classes, OLL(L/R) and PVD(L/R). ser-2prom1::gfp is expressed in the AIY interneuron class and a set of unidentified neurons. These neurons were identified as head and tail interneuron classes, namely AVHL/R, AUAL/R, AIYL/R, RICL/R, SABVL/R, RID, RIAL/R, SABD, SDQ, CANL/R, DA9, LUAL/R, ALNL/R, and PVCL/R. In addition to its expression in neurons, ser-2prom1::gfp is also expressed in pharyngeal cells (NSM neurons and pm1/6 muscles) and in head muscles. In males, expression can be observed in posterior dorsal and ventral body wall muscles, the male-specific diagonal muscles, and several posterior neurons likely to be CP neurons. |
|
|
|
|
Expr3021
|
Expressed in AIM, ASG, AVA, AVG, AVL, CEP, PVD, PVW, RIC/AIZ, RIV, SMD, URA, uv1. Male specific expression in 6 out of 9 CP. |
|
|
|
|
Expr11371
|
AFD (100%), AVK (87%), DVA (83%), NSM (22%), M5 (16%), RIC/AIZ? (16%), D-type motor neurons (33%), VC motor neurons (50%), CPs (20%), PDC (12%). Percentages correspond to the fraction of animals examined that expressed GFP in the indicated neurons. Above 50% marked as certain, below as uncertain. |
|
|
Reporter gene fusion type not specified. |
|
Expr959
|
Expressed in C. elegans serotonergic neurons: NSM, ADF, hermaphrodite-specific HSN, male-specific CP, and, rarely, AIM and RIH. tph-1::GFP expression in most of these neurons is observed by the L1 stage but expression in the late-maturing HSN and CP neurons is observed in the adult stage. |
|
|
This antibody recognize both bas-2 and tph-1, see Expr1235. Worms were prepared differently in Expr1235 and Expr1988 to highlight either hypodermal or neuronal staining. |
|
Expr1988
|
Observed many worms staining of cell bodies and processes of NSMs, identified as serotonergic neurons in the pharynx. Occasionally saw one or two small cells in the head in the location of other unknown serotonergic neurons. In a few hermaphrodites, identified a cell near the vulva located sublaterally and with a process extending ventrally as HSN. Also stained 6 neurons with processes in the ventral nerve cord, identified as CP neurons. No staining of dopaminergic neurons. |
Neuronal cell bodies and processes. |
