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Expr10756
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acr-18, which encodes a DEG-3 type acetylcholine receptor, is expressed in AVA. acr-18 reporter expression is not limited to AVA. In both sexes, acr-18 expression is apparent in a number of sex-shared head and ventral nerve cord neurons and, in the male, in a subset of ray and pre-anal ganglion neurons. |
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Expr1918
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In the pAB::GFP fusion, expression was seen in some pioneering neurones of the nerve ring, beginning at the early comma stage. At the two-fold stage, expression was detected in some 10 neurones in the head that extend axons into the nerve ring, and in two neurones in the tail that extend processes anteriorly. This expression pattern was confirmed by immunohistochemistry with MAb 16-48-2. At the three-fold stage, expression was seen in all DA motoneurones and persisted while they pioneered the dorsal nerve chord. It was also seen in four to six neurones in each of the four head ganglia, including ALA and RID in the dorsal ganglion, and four of the six neurones of the terminal bulb, including M5. In the tail, two neurones in the pre-anal ganglion and six in the lumbar ganglion, including PVQL and PVQR, showed pAB::GFP expression. Additionally, a transient expression was seen in the four rows of bodywall muscle cells in the embryo. After hatching, in L1 larvae, the expression domain extended to amphid and phasmid socket cells, and subsequently in L2 larvae to all the newly born AS motoneurones. In hermaphrodite L3 larvae, expression was seen in the sex myoblasts subsequent to their anterior migration towards the position of the presumptive vulva, and in adult worms at a high level in the vulval muscles vm1 and vm2. In males, expression was seen in the diagonal and spicule retractor muscles. |
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Expr1901
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The distribution of signal in transgenic worms was unique and highly restricted with respect to tissue and/or stage of development but did not correspond to the descendants of a particular branch of the cell lineage. Fluorescence was first detectable at the comma stage in cells that divided and appeared to migrate during the 2-fold and 3-fold stages. Neuronal staining was obvious from L1 onward and by early L4 was seen to occur in both the dorsal and ventral nerve chords. During this stage, a strong signal was noted in the developing vulva (most likely the vulE and/or vulF cells). By late L4 an intense GFP signal in the spermathecal valve as well as other vulval and/or uterine structures was evident. Expression in the uv1 and uv2 cells was suggested by the pattern of fluorescence around the vulva. However, the nuclear-localized reporter construct stained more nuclei than can be accounted for by expression in these cells alone. With this construct, nuclear localized signal was observed in all four nuclei of the syncytial spermathecal valve cell. Although GFP fluorescence was seen to be strongest in the late L4 and early adult for the spermathecal valve and vulval/uterine structures previously noted, it was seen to persist throughout adulthood. The M8 cell of the terminal bulb of the pharynx, all six cells of the pharyngeal-intestinal valve, and neuronal cell bodies within the metacorpus and around the isthmus of the pharynx also expressed gly-2p::GFP. At least 37 neurons with cell bodies lying next to the ventral nerve chord were positive for gly-2-directed reporter expression in the adult hermaphrodite, although with widely varying levels of staining. There was also GFP fluorescence present in other neurons associated with the preanal, dorso-rectal, and/or lumbar ganglia. In adult males, expression was similar in non-sexually dimorphic tissues and was also observed in axons that project into rays 2, 3, 5, 6, and either 8 or 9 of the copulatory bursa. |
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Expr1677
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Expression from the transgene is ubiquitous in somatic tissues including hypodermis, muscles, neurons, pharynx, gut, excretory canal, somatic gonad, vulva and presumably all cells. However, even using complex arrays to help prevent transgene silencing in the germline, no CLK-2::GFP expression could be detected in the germline. |
A full-length CLK-2::GFP fusion protein that complements the mutant phenotype for development, behavior and viability at 25C, is localized virtually exclusively to the cytoplasm. The pattern is unlikely to be a consequence of overexpression as very small transgene concentrations was used in complex arrays. However, although the nucleus appears dark in the fluorescent images, it is still possible that it contains very small amounts of the fusion protein. |
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Expr12962
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seb-3 is expressed in DVA, LUA, PVC, DVE, DVF, preanal ganglion motor neurons, in DVB and in one neuron of ray 1. |
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Expr13480
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