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Consistent with this observation, sequence analysis of ARI-1 using PSORT predicts ARI-1 to be a nuclear protein. |
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Expr3862
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Consistent with the embryonic expression pattern, ARI-1 is present at highest levels in muscle and neuronal cells in larvae and adults. Expression in muscle cells is observed at all stages including the body wall muscles, sex muscles, muscles associated with the posterior alimentary tract, and in lateral head and pharyngeal muscles. In addition, consistent expression were observed in a number of cells of the somatic gonad including distal tip, sheath, and spermathecal cells, as well as in vulval cells undergoing morphogenesis. Expression is also observed in head neurons, including many of the amphid neurons that are proximal to the posterior bulb of the pharynx and in neurons in the tail including DVA, DVB, and DVC. Neuronal expression in the mid-body includes the CAN, HSN, and ALM cells; neurons of the ventral and dorsal cords; and a number of posterior deirid neurons. Lastly, expression is observed in all pairs of coelomocytes Expression of ARI-1 is dynamic during early embryonic development, with ubiquitous somatic expression occurring between the 50- and 200-cell stage. By the late proliferative phase of embryogenesis (~400 - 500 cells), expression is reduced in most nuclei but is maintained at high levels in muscle precursors. Similar to later embryonic and larval time points, expression in the early embryo is always observed in both the nucleus and cytoplasm of GFP-positive cells. Following the proliferative phase of embryogenesis, ARI-1 embryonic expression is most pronounced in muscle precursors with moderate expression also occurring in the lateral ectoderm, which gives rise to many neurons in addition to hypodermal cells. Notably, pharyngeal expression of ARI-1 is very low at both the comma and 1.5-fold embryonic stages, during which time pharyngeal morphogenesis and attachment are occurring. |
Expressed in nuclei. |
