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Expr16133
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The measurement of transcript accumulation showed that the two putative parvulin genes are expressed at relatively low levels throughout development. |
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Expr13949
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The strongest fluorescence was observed in the pharyngeal muscles, and the signal was widely distributed in the whole body of adult animals, including the intestine, body wall muscles and hypodermis. Y82E9BR.3p::gfp was expressed in all developmental stages, including eggs and larvae. |
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Expr14927
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A functional single-copy TRAP-1::mCherry fusion protein generated by CRISPR-Cas9-mediated knock-in is expressed in several tissues in C. elegans embryos, larvae, and adult animals. |
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Don't dump for LEGO CAM (capturing function in GO) |
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Expr12818
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TCER-1::GFP was visible at all stages of embryonic and larval development. In adults, we observed strong nuclear localization of TCER-1::GFP in intestinal cells, many head and body neurons, muscle and hypodermal cells. In some intestinal cells, weak expression was also observed in the cytoplasm. |
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Expr15168
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affl-2::GFP was visible in all tissues in worms of all stages, which indicates that affl-2 is ubiquitously expressed. |
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Expr12451
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iffb-1 mRNA was detected by in situ hybridization at all developmental stages and was particularly abundant in early embryos and in the germ line of L4 larvae and adults. The iffb-1 mRNA expression pattern has been characterized by the Nematode Expression Pattern Database consortium (NEXTDB). |
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Expr15209
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Using a novel manf-1 reporter allele, we confirmed that the MANF protein is expressed throughout development and is expressed in most somatic cells in L1 larvae, which is the developmental stage that we focused on in the current study. |
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Expr11922
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gcn-1 was expressed in most cells during all stages of development. gcn-1 expression was observed in head neurons, hypodermal cells, intestinal cells, body wall muscles, and pharyngeal neurons, including the M4 neuron. |
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Expr15190
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NFYB-1 endogenously tagged with mKate2 was nuclear localized in tissues throughout the body, including neurons, hypodermis, muscle, intestine and germline. Expression was found from embryo to adult, and levels in the adult peaked at day 5 and then declined thereafter. |
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Expr14784
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In all three cases, each reporter showed very broad if not ubiquitous, nuclear expression throughout development. |
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Expr15555
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Using NeuroPAL, we confirmed sIs14542 expression in AVH, but not AVJ. We further corroborated hlh-34prom expression in AVH by observing that in the embryo, expression of otIs768 is first transiently observed in 4 cells, two of which show signs of cell death; later in embryogenesis, and then during all stages of postembryonic and adult development, expression becomes restricted to 2 cells. This is consistent with expression in the bilateral AVH neuron pair, since their two sisters cells are, unlike the sisters of the AVJ neuron pair, destined to die by apotosis (Sulston et al. 1983). |
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Expr16356
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Transgenic analysis revealed that the hrg-9 transcriptional reporter (hrg-9p::gfp) was expressed predominantly in the worm intestine throughout all developmental stages. |
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Expr16410
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Expr11622
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Expression of ceh-34::gfp transgene began during embryogenesis. CEH-34::GFP was localized to the nuclei of expressing cells. During embryonic morphogenesis and larval development and throughout adulthood, expression of the ceh-34::gfp transgene was seen predominantly in pharyngeal cells. The ceh-34::gfp transgene was expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2, I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2) and pharyngeal epithelial cells (e1 and e3), and some body wall muscles around the anterior pharynx. |
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Expr1431
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In six comma-stage embryos, an average of 80.5 ( 2.5 s.d.) PHA-4-staining pharyngeal nuclei were counted by confocal microscopy (maximum count = 85). Analysis of later stage animals shows clearly that all six cells of the pharyngeal-intestinal valve contain PHA-4 protein. Thus the total number of potentially staining nuclei (pharynx + valve) should be equal to 86. all cells of the pharyngeal primordium (+ valve) contain the nuclear factor PHA-4. The same embryos also show nuclear PHA-4 in 6-8 rectal cells, including the two rectal valve cells and the three rectal epithelial cells. At the lima bean and comma stages of embryogenesis, a low but significant level of PHA-4 can be detected in the gut. pha-4 gene is expressed in pharyngeal precursor cells well before the formation of the pharynx primordium. PHA-4 can be detected immunologically in most but not all nuclei of the larval and adult pharynx. PHA-4 is detected in nuclei of all epithelial cells, muscle cells, marginal cells, gland cells and pharyngeal intestinal valve cells, but is detected only in about 8 of 19 neuronal nuclei. PHA-4 is also detected in one (comma stage) to several (pretzel stage and later) head cells outside of the pharynx. PHA-4 can also be detected in nuclei of the developing somatic gonad, including the distal tip cell and ventral uterine cells. |
nuclear |
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See Expr837, 839, 840 for Expr_pattern of the same locus. |
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Expr838
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In embryos, PEB-1 protein was strongly detected in the nuclei of many cells in the developing pharynx. Staining was first detected at the comma stage. Some pharyngeal cells do not express PEB-1, observed a cluster of nuclei that do not contain PEB-1 near the center of the developing pharynx, a region containing many of the pharyngeal neurons. Strong pharyngeal staining persisted through the remainder of embryogenesis. PEB-1 staining was also observed outside the pharynx in several cells near the rectum and in the tail and at lower level in hypodermal nuclei. In early larvae, PEB-1 protein is easily detectable in the pharynx, as well as in a subset of nonpharyngeal. Consistently observed PEB-1 protein in the nuclei of all pharyngeal muscles, marginal cells, epithelial cells, gland cells, and pharyngeal-intestinal valve cells. Notably, authors did not observe PEB-1 staining in any pharyngeal neurons. Pharyngeal PEB-1 staining decreased in late larvae until it became undetectable in adults. PEB-1 protein expression was also observed in some tissues outside the pharynx in larvae and adults. In general, the nonpharyngeal expression was weaker than expression in the pharynx, and it appeared dynamic during the worm life cycle. In larvae, PEB-1 antibody staining was reproducibly observed in several cells near the rectum and in hypodermal nuclei in the tail and in the developing vulva and surrounding cells. Authors also observed protein expression in several nuclei in the head and tail that were provisionally identified as hypodermal nuclei. In older larvae and adults, the rectal and vulval expression decreased, but PEB-1 staining was visible in germ-line cells. This expression is consistent with Northern blots indicating that peb-1 mRNA is expressed in the germ line. |
PEB-1 protein was strongly detected in the nuclei. |
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Expr12161
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Transgenic worms containing the smf-3 pro::gfp construct demonstrated strong GFP expression at all stages of development, beginning as early as the comma stage embryo and continuing through larval and adult stages. Expression was spatially confined to intestinal cells, excretory cells, vulval epithelial cells, and neuronal cells. Fluorescence was largely observed at the apical ends of the adult and larval intestines; in neuronal cells, particularly in the head neurons and hypodermis; in H-shaped excretory cells; and in vulval epithelial cells. Worms expressing the smf-3 pro::gfp construct exhibited distinct intestinal expression, which encompassed most of the intestine. |
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Expr1682
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AJM-1 localizes to the apical borders of all C. elegans epithelia. Expression occurs in the embryonic hypodermis, pharynx and intestine, as well as in post-embryonic epithelia, including the vulva, uterus, spermathecae, pharynx, intestine, hindgut, hypodermis and male tail. MH27 staining of embryos expressing HMP-1GFP reveals that AJM-1 occupies an apical domain that is basal to the HMRHMP(cadherincatenin) complex in all epithelial tissues examined. Transmission electron microscopy (TEM) analysis of embryonic hypodermal cell junctions revealed an apical junctional domain resembling adherens junctions of flies and vertebrates, whereas no morphologically distinct septate or tight junctions are observed. The apicobasal extent of this domain is ~100 nm. Immuno-TEM analysis of hypodermal cells of larvae with the MH27 antibody revealed that AJM-1 specifically localizes to the apical domain. |
AJM-1 localizes to the apical borders of all C. elegans epithelia. |
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Expr1499
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DLG-1::GFP was first detected at the 350-cell stage in differentiating epithelial cells and neuroblasts. The neuronal expression was transient. After ventral enclosure, DLG-1::GFP was detected in the epidermis, pharynx and intestine, forming a continuous belt around epithelial cells at a subapical position, which persists throughout embryonic, larval and adult development. In adults, the DLG-1::GFP protein was also detected in epithelial cells contributing to the reproductive system: the vulva, uterus and spermatheca. |
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Expr13334
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In hermaphrodites and males, selt-1.1 GFP expression was observed in neurons, epithelial and muscle cells of transgenic animals carrying both transcriptional (i.e. selt-1.2 promoter driving GFP expression) and translational (i.e. containing selt-1.2 promoter, exons and introns driving GFP expression) constructs. Crosses with the pan-neuronal marker rab-3 fused to the red fluorescent protein (RFP) in the nuclei confirmed that SELT-1.1 is expressed in all neurons of the nervous system. The expression of selt-1.1 in the ADL, ASH, ASI, ASJ, ASK, AWB amphid sensilla neurons was also confirmed by DiI staining. In the epithelia, selt-1.1 is expressed in the hypodermal, arcade, pharyngeal, vulval and rectal cells. selt-1.1 was not found to be expressed in the intestine or in the gonad. Muscle cells expressing selt-1.1 include the somatic muscle cells from head, neck and body wall as well as the non-striated pharyngeal muscles. SELT-1.1 expression was observed throughout development, from pre-bean embryonic stages to the adult stage. Embryos expressed GFP in most cells, also with a perinuclear localization. |
The translational Pselt-1.1::selt-1.1::gfp reporter revealed perinuclear localization, consistent with the ER localization previously reported for mammalian SELENOT. The ER localization of SELT-1.1 was confirmed by expressing into the QW1266[Pselt- 1.1::selt-1.1::gfp] the ER marker tram-1 fused to mcherry in muscle cells. |
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Expr14889
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hrpk-1::gfp is ubiquitously expressed during all C. elegans developmental stages. The C-terminally tagged hrpk-1::gfp expression is observed in the gut, muscle, neuronal, and hypodermal tissues, where it localizes to the cell nuclei. |
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Expr1330
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In hermaphrodites, GFP is not observed in gonads from first-stage(L1) larvae. Its first gonadal expression occurs in L2 and is limited to the DTCs. GFP continues to be expressed in DTCs through L4, but is faint or not detectable in the adult. GFP is similarly expressed in the male linker cell. In addition to its expression in leader cells, the gon-1 promoter also drives GFP in muscle cells throughout development. |
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Reporter gene fusion type not specified. |
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Expr1337
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Pers Comm. from Rachel Aronoff 11-29-01: clear expression in adult muscle and in embryonic cells of many lineages, including myocytes. |
SMG-4:GFP localizes both to nuclear and cytoplasmic compartments. Confocal microscopic resolution, furthermore, reveals bright puncta both around nuclei and extending into the cytoplasm. In the embryo, GFP luorescence is visible both in the nucleus and cytoplasm of many cells, although the nuclear signal is stronger. Intense GFP puncta can also be seen in or around the nucleus. From successive confocal series through individual nuclei, the very bright GFP puncta appear to be perinuclear, as the signal can sometimes be seen radially distributed around the nucleus. Such perinuclear accumulation can also occasionally be seen by standard fluorescence microscopy. However, in the confocal microscope, additional bright GFP puncta extending into the cytoplasm are also frequently evident. |
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Expr15998
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LPLA-2:: CHERRY was widely expressed from embryonic stages throughout larval and adult stages in various tissues, including pharynx, hypodermis, sheath cells, intestine, muscle and tail region. |
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Expr15523
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pry-1 expression revealed expression in almost all tissues during development. Expression in seam cells, neuronal cells, muscles, hypodermis, and intestine was readily visible. The most enriched tissues include neurons and muscles. A closer examination of GFP localization in developing animals revealed bright fluorescence in the ventral cord region, which includes neuronal and non-neuronal cells. The expression was largely similar in adults, although the fluorescence was much higher in BWMs. The posterior end of the intestine, near the rectal opening, showed a strong signal in L4 and adult animals; however, the rest of the intestine lacked a detectable expression. |
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Expr9816
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GFP expression was observed from early embryogenesis through to the adult. In the L1/L2, many nerve cells in the nerve ring, elsewhere in head and in the tail expressed GFP, as well as many other cells in the head, in the hypodermis and/or muscle. GFP expression was also observed in the intestine in some larvae and adults but this was much weaker and less consistent than other components. Additionally, strong expression in some other unidentified cells in the centre of the body of L1/L2s was seen inconsistently. The GFP remained in some neurons at high levels into adulthood. |
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Expr9835
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Broad GFP expression was observed from early embryogenesis to the mature adult. Particularly high levels of GFP were seen in the L2/L3 stages, in possibly all nuclei in the head, but certainly in many neuronal, hypodermal and muscle cells. GFP was also expressed in the larval hypodermis down the rest of the body. GFP was seen in the intestine throughout development. High levels of GFP were observed in the developing reproductive system, particularly in the spermathecae. GFP levels were generally lower in adults, apart from a few neurons in the head with very high levels, but GFP was still present in the intestine, in some neurons in the tail and possibly in body wall muscle in younger adults. |
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Expr9845
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Broad GFP expression was observed from early embryogenesis through to the mature adult. Expression pattern components included neurons in head, tail and ventral nerve cord, cells in the head that are probably muscle cells, vulval and uterine muscle, and in the body wall, in the hypodermis and possibly muscle. Some weak intestinal GFP expression was seen occasionally but inconsistently. |
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Expr1449
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PKC3 is expressed throughout the lifespan of C. elegans. However, both the content and intracellular distribution of PKC3 vary with development. For example, total PKC3 content in L1 larvae is increased 7-fold relative to the minimal level observed in L3 animals. Embryos and L4 larvae are also 3- to 4-fold enriched in PKC3. |
A very high proportion (75100%) of PKC3 partitions with the insoluble fraction of homogenates from embryos, L2L4 larvae, and young adult C. elegans. L1 larvae have the highest level of particulate PKC3 but also contain a similar amount of the kinase in cytosol. The highest level of soluble PKC3 is detected in L1 larvae. |
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To compare the transcription pattern of the daf-1 promoter with the whole gene, the gfp cDNA was fused to the terminus of the daf-1 gene in a plasmid that included 2.6 kb of upstream sequence. Despite the fact that this transgene rescued the daf-1 Daf-c mutant phenotype, GFP fluorescence was not detected in rescued animals. Hence, these observations were limited to the daf-1 promoter fusion, which may not represent the expression pattern of the whole daf-1 gene if enhancer elements are present in introns or in 3' sequences. |
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Expr946
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GFP expression was observed in the head and the developing ventral nerve cord beginning in mid-stage embryos and continuing into adulthood. In the head, GFP was detected in more than twenty neurons in the anterior, lateral, ventral and retrovesicular ganglia. Fluorescent processes terminating at the tip of the head suggest that daf-1 is expressed in sensory neurons and in support cells in the amphids and inner labial sensilla. In the midbody, GFP was expressed in the ALM mechanosensory neurons and the PVT neuron, as well as one additional neuron pair. In the lumbar ganglia of the tail, five cells expressing GFP included phasmid neurons and PLN and PLM mechanosensory neurons. The daf-1 promoter also conferred gfp expression in nonneuronal cells, including a membranous sheath surrounding the distal end of the intestine and in the distal tip cell (DTC) of the gonad. In some lines, GFP was sometimes detected in the muscles of L4 and adult animals. In L1 larvae, daf-1 promoters is active in neurons in the head, as well as in the ventral cord and tail. The promoter continues to express GFP in dauer larvae from starved plates. |
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