|
|
|
Expr14540
|
zmp-5 is expressed in the dorsal uterine cells above the AC. |
|
|
|
|
Expr2276
|
Neuronal expression. In the L1 stage cog-1::gfp is expressed in amphid neurons ADL(L/R), ASE(L/R), and ASJ(L/R). One additional pair of head neurons expresses cog-1::gfp in the L1. This pair is located between the excretory cell and the excretory pore, and is probably either AIA(L/R), SMBD(L/R), SIAD(L/R), or SIAV(L/R). In the tail, phasmid neurons PHB(L/R) express cog-1::gfp. The expression in amphid and phasmid neurons persists to the adult. Additional unidentified cells in the preanal ganglion express cog-1::gfp in late larvae and adults. Other cells in the tail region. The sphincter muscle (mu_sph) and phasmid sheath cells (PHshL, PHshR) express cog-1::gfp in all stages examined. Uterine expression. In the hermaphrodite gonad, cog-1::gfp is expressed exclusively in the dorsal uterine lineage. During the L3 stage, GFP is in the central four great-granddaughters of DU cells (DE4/DE5; DE, dorsal eight). The expression apparently persists in DE4/DE5 descendants, du, uv2, and uv3, until the adult stage, DE2 and DE7 lineages in the dorsal uterus also express cog-1::gfp starting from the early L4 stage. In the late L4, cog-1::gfp in this region is observed exclusively in sujc cells. Each core is surrounded by the spermathecaluterine valve and appears to form a plug which blocks the uterine lumen from the spermathecal lumen. None of the cog-1::gfp fusions, including the rescuing construct, was ever observed to express in any ventral uterine cells, including the anchor cell. Thus, the function of cog-1 in the connection is believed to be a consequence of abnormal gene regulation in the vulva, although a function in the dorsal uterine cells have not been ruled out. Vulval expression. In the vulva, cog-1::gfp is expressed from early L4 to the adult. Cells that express are vulC [P5.ppa(l/r), P7.pap(l/r)], vulD (P5.ppp, P7.paa), vulE(P6.pppl, P6.pppr, P6.paal, P6.paar), and vulF (P6.ppal, P6.ppar, P6.papl, P6.papr). The expression is considerably brighter in vulC and vulD than in vulE and vulF. Occasionally, expression in vulB and vulA was observed. Male expression. The expression of cog-1::gfp in the male was scored mostly in animals containing an extrachromosomal array containing the SmaI insertion. cog-1::gfp is expressed during proctodeal development. eL.aav and eR.aav express cog-1. In addition, proctodeal cells B.pap and B.pppa express cog-1::gfp. Additional expressing cells observed in occasional males include: rep, and P11.pp progeny. No gonadal cells in the male, including the linker cell, expressed cog-1::gfp in any stage of development. |
|
|
|
|
Expr9649
|
VAB-23::GFP was expressed in the AC, the vulval cells, in the ventral and dorsal uterine cells, the seam cells, the vulval muscle cells, a small cluster of unidentified tail cells, and some ventral cord neurons. Vulval expression of VAB-23::GFP was observed predominantly in the 1° lineage beginning at the time of induction and persisting until adulthood. Even thoughVAB-23::GFP was initially expressed at low levels in all VPCs, expression was downregulated in the 2° lineage during induction and persisted at low levels in the tertiary (3°) cells. VAB-23::GFP continued to be strongly expressed in the VulE and VulF cells of L4 larvae during vulval morphogenesis, although relatively weaker expression was also observed in VulC and VulD at this later stage. |
|
|
Expression pattern at adult stage was not described. |
|
Expr1410
|
Expression in Z1 and Z4, the somatic gonad precursors, starts a few hours after these are born. Up to the formation of the somatic gonad primordium in late L2, LIN-26 protein was detected in all cells of the somatic gonad, except in the distal tip cells or dtcs (Z1.aa and Z4.pp). Staining was strongest in Z1 and Z4 and became weaker after each cell division. However, after Z1.a and Z4.p divided, LIN-26 was initially not detected in any of their daughters, but resynthesis occurred in Z1.ap and Z4.pa. During the L3 stage, expression was only detected in the anchor cell (AC) until the Pn.pxx cells were generated. After the last division round in L4 larvae, all uterine nuclei and two spermathecal-uterine junction nuclei expressed LIN-26 at very low levels. |
nuclei |
|
|
|
Expr15693
|
In mid-L2 larvae, SRA-9::GFP was expressed in the AC and other uterine cells but not in the VPCs or the VNC. From the early L3 stage on, SRA-9::GFP expression was also observed in the VPCs, whereas AC expression faded until it was absent in mid-L3 (Pn.px stage) larvae. |
|
|
Reporter gene fusion type not specified. |
|
Expr1814
|
ayIs9 expresses GFP in a subset of cells within the somatic gonad. These GFP cells are the dorsal uterine (DU) cells based on their positions and division patterns. Furthermore, GFP expression was abolished in all animals in which the DUs were ablated (n=10), but could always be observed in control animals in which the DUs were left intact (n=25). Based on these results, EGL-17 appears to be expressed in the somatic gonad by the DU cells. |
|
|
|
|
Expr13257
|
Wild-type PAT-3::GFP was expressed on the lateral membranes of the vulval cells and along the basal laminae that separate the vulval cells from the uterus. Since PAT-3::GFP was also expressed in the adjacent uterine cells, both tissues are likely to contribute to the strong signal along the basal laminae. PAT-3::GFP localization on the lateral membranes of the vulval cells was most prominent at the onset of vulval invagination in mid to late L3 larvae. |
|
