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Expr12671
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In males, PLC-3 was previously reported to be expressed in the seminal vesicle valve cell and the vas deferens. A larger upstream promoter region - 4.4 kb plc-3 promoter construct- also expressed in the ALA neuron, the male retractor muscles, and the ventral protractor muscles. |
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Expr9862
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The primary site of try-5 expression was in the male somatic gonad, in particular within tissues involved in storing sperm and tissues through which sperm pass during transfer to a hermaphrodite. Starting at the L4 larval stage, when sperm production initiates, we observed Ptry-5::GFP::H2B expression in several regions of the male gonad: the seminal vesicle (up to seven of the twenty-three total cells in this tissue), the valve region (four cells), and the twelve cuboidal cells of the vas deferens. This overall pattern persisted into adulthood until at least 72 hr post L4; highest expression levels were present consistently in the valve region. We observed no expression in the hermaphrodite gonad, so gonadal expression of try-5 is sexually dimorphic. However, we also observed low levels of expression in a few cells within the head and tail of both males and hermaphrodites. |
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Expr3702
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Expression in male-specific structures is directed by all three promoters. Promoter pA directs expression in the spicule protractor muscles of the proctodeum and in a single male-specific neuron that is likely to be CP8 or CP9. Promoter pB directs expression in the spicule retractor muscles, gubernaculum retractor muscles, posterior oblique muscles, diagonal muscles, and the vas deferens. Promoter pC also directs expression in the vas deferens, as well as the seminal vesicle and the valve that separates the seminal vesicle and vas deferens. Thus itr-1 is expressed widely in the sex-associated muscles and somatic gonad of males. |
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Expr12669
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plc-3 (encoding PLC-gamma) is expressed in the vas deferens and in the valve cell that separates the seminal vesicle and vas deferens. |
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Expr9863
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In worms carrying the Ptry-5::TRY-5::GFP reporter, the TRY-5::GFP fusion protein exhibited a localization pattern consistent with secretion from the vas deferens. Within the valve and cuboidal cells, TRY-5::GFP was localized to globular foci. In L4 larvae, most globules aligned with the apical domain that lines the developing sperm channel. In mature adults, very large globules were present that tended to cluster apically, and additional small globules were present throughout the cytoplasm. We sometimes observed TRY-5::GFP within the lumen of the seminal vesicle, likely as a result of release from the adjacent, highly-expressing valve cells. |
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Expr13035
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B0207.5 is expressed in the valve region of the vas deferens. |
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