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Expr1200380
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Data from the TransgeneOme project |
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Expr1200040
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Data from the TransgeneOme project |
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Expr1200070
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Data from the TransgeneOme project |
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Expr1200089
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Data from the TransgeneOme project |
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Expr1200129
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Data from the TransgeneOme project |
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Expr12704
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GFP::LEP-2 is expressed throughout development from embryo to adult. It is seen in many tissues, including seam-, tail tip- and other hypodermal cells, head and tail neurons, pharynx, intestine, and the developing hermaphroditic somatic gonad. Expression seems to be excluded from body muscle cells. |
The reporter is diffusely expressed in the cytoplasm but absent from nuclei. |
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Expr1200002
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Data from the TransgeneOme project |
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Expr1200109
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Data from the TransgeneOme project |
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Expr1200115
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Data from the TransgeneOme project |
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Expr1200035
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Data from the TransgeneOme project |
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Expr12246
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lin-4 was temporally expressed in the hypodermal seam cells at the early L2 stage and expression persisted in adulthood. Furthermore, the spatial patterning of lin-4 in the hypodermal seam cells correlated with the requirement of this gene at the L1/L2 transition to direct normal seam cell development.lin-4 expression was first observed within the developing gonad at the beginning of L3, specifically in the distal tip cells and the anchor cell of the somatic gonad and expression in the distal tip cells continued in the adult. lin-4 and let-7 were weakly expressed in the anchor cell, which is required for proper vulval patterning, and in the P5.p, P6.p, and P7.p cells that will later differentiate into the mature vulva. |
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Expr12249
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mir-237, was not expressed in the seam cells until eL3, which mirrored the up-regulation of this gene at this stage by northern blot. mir-237, was detected two stages earlier at L1 in the somatic gonad progenitor cells, Z1 and Z4. This result differs from our northern blot data, which showed that miR-237 RNA was first detected at L2 (Expr3739). We believe that the mir-237::gfp expression at the L1 stage is accurate, and since mir-237 is only expressed in the Z1 and Z4 cells at this stage, miR-237 RNA is below the levels of detection by northern blot analysis. Once the Z1 and Z4 somatic gonad derivatives expand at L2, miR-237 RNA can be seen by northern blot. By the L3 stage, and persisting into the adult, mir-237 was expressed in the developing gonad, including the anchor cell, a subset of unidentified uterine cells, and the distal tip cells. The up-regulation of mir-237 expression in animals carrying the mir-237::gfp construct at the L3 stage in a variety of tissues, such as the gonad and the hypodermis, mirrors the increased miR-237 RNA levels observed at L3 by northern blot analysis. In addition, mir- 237 and mir- 84, were both expressed in the anchor cell and in the VPCs except P6.p at the mL3 stage. It is interesting to note that expression of mir-237, mir-84, and mir-48 was absent in the P6.p cell during the mid- to late L3 stage, and expression of mir-237 and mir-84 but not mir-48 was present in the anchor cell. |
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Expr1200008
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Data from the TransgeneOme project |
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Expr9768
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Weak, nuclear-localized GFP in the developing gonad in larval stages. |
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Expr9746
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GFP expression was seen in a few cells of the developing gonad from L1-L4. No head or tail neuronal expression, as with the egl-13 reporter gene fusion arrangements present in UL3393 or UL3196, was seen. |
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Expr1200104
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Data from the TransgeneOme project |
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Expr1200064
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Data from the TransgeneOme project |
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