WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

Expression Pattern :

Pattern  The transgene containing pos-1 3' UTR expressed the GFP::H2B reporter strongly in oocytes, but not in mitotic or early pachytene regions. Primary Identifier  Expr9715
Reporter Gene  We employed a transgene-based assay that tests the post-transcriptional control activity of 3' UTRs in vivo. In this assay, the GFP::H2B reporter fusion is placed upstream of the selected 3' UTR such that the entire fusion-GFP::H2B-3' UTR-is produced as a single transcript. Expression in the germline is achieved by using pie-1 promoter, which has been shown earlier to drive transgene expression in the germline.

1 Anatomy Terms

Definition Name Synonym Primary Identifier
a female haploid germ cell. oocyte ovum WBbt:0006797

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00004078 pos-1 F52E1.1 Caenorhabditis elegans

0 Life Stages